中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
8期
1686-1687
,共2页
匹立尼酸%肾脏缺血%再灌注损伤%肿瘤坏死因子-α%白细胞介素-1β
匹立尼痠%腎髒缺血%再灌註損傷%腫瘤壞死因子-α%白細胞介素-1β
필립니산%신장결혈%재관주손상%종류배사인자-α%백세포개소-1β
Pirinixic acid%Renal ischemia%Reperfusion injure%Tumor necrosis factor-α%Interleukin-1β
目的 观察匹立尼酸(PA)对肾脏缺血再灌注大鼠血清肿瘤坏死因子-α(TNF-α)和白细胞介素-1β(IL-1β)的影响.方法 健康雄性SD大鼠54只,随机均分为3组:假手术组(S组)、缺血再灌注组(I/R组)、PA组.在肾缺血60 min后再灌注建立大鼠缺血再灌注肾损伤模型,S组操作同上但不阻断血管.缺血前30 min,PA组经腹腔注射PA 5 mg/kg,I/R组和S组注射等容积生理盐水.4h后取腹主动脉血检测血清TNF-α和IL-1β的水平.结果 再灌注4h后,I/R组血清TNF-α和IL-1β水平分别为(11.65±1.15) ng/L和(230.80 ±31.82) ng/L,PA组为(7.83±1.27) ng/L和(125.74±18.03) ng/L,PA组显著低于I/R组(P＜0.05).结论 在缺血再灌注肾损伤中,预先给予PA可起到降低血清TNF-α和IL-1β水平的作用.
目的 觀察匹立尼痠(PA)對腎髒缺血再灌註大鼠血清腫瘤壞死因子-α(TNF-α)和白細胞介素-1β(IL-1β)的影響.方法 健康雄性SD大鼠54隻,隨機均分為3組:假手術組(S組)、缺血再灌註組(I/R組)、PA組.在腎缺血60 min後再灌註建立大鼠缺血再灌註腎損傷模型,S組操作同上但不阻斷血管.缺血前30 min,PA組經腹腔註射PA 5 mg/kg,I/R組和S組註射等容積生理鹽水.4h後取腹主動脈血檢測血清TNF-α和IL-1β的水平.結果 再灌註4h後,I/R組血清TNF-α和IL-1β水平分彆為(11.65±1.15) ng/L和(230.80 ±31.82) ng/L,PA組為(7.83±1.27) ng/L和(125.74±18.03) ng/L,PA組顯著低于I/R組(P＜0.05).結論 在缺血再灌註腎損傷中,預先給予PA可起到降低血清TNF-α和IL-1β水平的作用.
목적 관찰필립니산(PA)대신장결혈재관주대서혈청종류배사인자-α(TNF-α)화백세포개소-1β(IL-1β)적영향.방법 건강웅성SD대서54지,수궤균분위3조:가수술조(S조)、결혈재관주조(I/R조)、PA조.재신결혈60 min후재관주건립대서결혈재관주신손상모형,S조조작동상단불조단혈관.결혈전30 min,PA조경복강주사PA 5 mg/kg,I/R조화S조주사등용적생리염수.4h후취복주동맥혈검측혈청TNF-α화IL-1β적수평.결과 재관주4h후,I/R조혈청TNF-α화IL-1β수평분별위(11.65±1.15) ng/L화(230.80 ±31.82) ng/L,PA조위(7.83±1.27) ng/L화(125.74±18.03) ng/L,PA조현저저우I/R조(P＜0.05).결론 재결혈재관주신손상중,예선급여PA가기도강저혈청TNF-α화IL-1β수평적작용.
Objective To investigate the effects of pirinixic acid (PA) on serum tumor necrosis factor-α (TNF-α) and interleukin-lβ (IL-1β) in rats with renal ischemia-reperfusion (I/R) injury.Methods Fifty-four male SD rats were equally randomized into 3 groups:sham operation group (S group),I/R group and PA 5 mg/kg + I/R group (PA group).The renal I/R injury model in rats was established by clamping blood supply of the kidney with vascular clamps for 60 min.Rats in S group were subjected to the same surgical procedures without clamping blood vessels.Rats in PA group were intraperitoneally administrated with the dose of PA 5 mg/kg 30 min before ischemia.In S group and I/R group,rats were given the same volume of saline at the same time.Blood samples were taken from abdominal aorta 4 h after reperfusion to measure the serum levels of TNF-α and IL-1β.Results Four h after renal I/R,the serum levels of TNF-α and IL-1β were (11.65 ± 1.15) ng/L and (230.80 ±31.82) ng/L in I/R group,and (7.83 ± 1.27) ng/L and (125.74 ± 18.03) ng/L in PA group.Group PA had lower serum levels of TNF-α and IL-1β than I/R group (P ＜ 0.05).Conclusion PA preconditioning could reduce the serum levels of TNF-α and IL-1β in rats with renal I/R injury.
