CAJ | 학술논문

目的探讨Pokemon白身抗体在原发性肝癌(Hcc)中的表达及其作为诊断标志物的意义.方法应用BL2l(E3)大肠杆菌表达并经镍柱纯化Pokemon抗体,Western blot证实具有免疫原性,应用酶联免疫吸附试验(ELISA)方法筛选142例HCC 、30例慢性肝炎、28例肝硬化及96例正常人血清中Pokemon自身抗体的表达水平,并与甲胎蛋白(AFP)水平比较.结果纯化的Pokemon蛋白能够被特异抗体识别.HCC中抗Pokemon抗体阳性率(49％)显著高于肝炎(0)、肝硬化患者(0)及正常人(0)(P＜0.01).HCC中抗Pokemon抗体的平均滴度(0.51±0.03)也明显高于肝炎(0.18±0.05)、肝硬化患者(0.16±0.03)及正常人(0.17±0.01)(P＜0.01).Pokemon自身抗体阳性率与AFP阳性率(＞200 μg/L)呈显著负相关(P＜0.01).结论 HCC患者血清检测Pokemon抗体水平能够补充AFP检测结果的不足之处,有助于诊断肝癌.
목적 탐토Pokemon백신항체재원발성간암(Hcc)중적표체급기작위진단표지물적의의.방법 응용BL2l(E3)대장간균표체병경얼주순화Pokemon항체,Western blot증실구유면역원성,응용매련면역흡부시험(ELISA)방법사선142례HCC 、30례만성간염、28례간경화급96례정상인혈청중Pokemon자신항체적표체수평,병여갑태단백(AFP)수평비교.결과 순화적Pokemon단백능구피특이항체식별.HCC중항Pokemon항체양성솔(49％)현저고우간염(0)、간경화환자(0)급정상인(0)(P＜0.01).HCC중항Pokemon항체적평균적도(0.51±0.03)야명현고우간염(0.18±0.05)、간경화환자(0.16±0.03)급정상인(0.17±0.01)(P＜0.01).Pokemon자신항체양성솔여AFP양성솔(＞200 μg/L)정현저부상관(P＜0.01).결론 HCC환자혈청검측Pokemon항체수평능구보충AFP검측결과적불족지처,유조우진단간암.
Objective To investigate the prevalence of autoantibodies against Pokemon in hepatocellular carcinoma (HCC) and its significance as a serological diagnostic biomarker.Methods Recombinant Pokemon protein was expressed from E.coli BL21 (E3) and was purified using nickel column chromatography.The antigenicity of the purified Pokemon protein was performed by Western blotting.Autoantibody responses to Pokemon were evaluated by enzyme-linked immunosorbent assay (ELlSA) in sera from 142 patients with HCC,28 with liver cirrhosis (LC) and 30 with chronic hepatitis (CH),as well as from 96 normal human individuals.Results The antigenicity of the purified Pokemon protein was confirmed and the recombinant Pokemon was able be used as coated protein in ELISA assay.The prevalence of autoantibodies against Pokemon was 49％ (69/142) in HCC,which was significantly higher than that in LC (0),CH (0) and normal hnman sera(0) (NHS; P ＜0.01).The average titer of autoantibodies against Pokemon in HCC sera(0.51 ±0.03) was higher compared to that in LC (0.16 ±0.03),CH (0.18 ±0.05) and NHS(0.17 ± 0.01) (P ＜ 0.01).When both autoantibodies against Pokemon and alpha fetal protein (AFP) were used simultaneously as diagnostic markers,sensitivity reached 71.12％.Conclusion Anti-Pokemon autoantibodies may be potential diagnostic markers for HCC,especially in conjunction with AFP.