中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
8期
1720-1722,封4
,共4页
增殖细胞核抗原%肝细胞癌%表达%临床研究
增殖細胞覈抗原%肝細胞癌%錶達%臨床研究
증식세포핵항원%간세포암%표체%림상연구
Proliferating cell nuclear antigen%Hepatocellular carcinoma%Expression%Clinical study
目的 探讨增殖细胞核抗原(PCNA)在肝细胞肝癌的发生发展中的作用及作用机制.方法 采用逆转录-聚合酶链反应(RT-PCR)和免疫组织化学法检测PCNA在肝细胞癌、相应癌旁组织和正常肝组织中mRNA和蛋白质的表达,正常肝组织、癌旁组织、肝细胞癌组织中的阳性表达率分别为20.0％、46.2％和85.7％；结合患者的年龄、性别、肿瘤大小、分化程度、有无淋巴结转移等临床病理学资料进行统计学分析,其表达与组织分化程度和淋巴结转移呈明显相关(P＜0.05).结果 PCNA在正常肝组织、癌旁组织、肝细胞癌组织中的阳性表达率分别为20.0％、46.2％和85.7％,在肝细胞癌中的阳性表达率显著高于正常肝组织和癌旁肝组织(P＜0.05)；RT-PCR检测结果也证实PCNA mRNA在肝细胞癌中的表达明显高于正常肝组织和癌旁肝组织(P＜0.05).PCNA的表达与患者年龄、性别、肿瘤大小等因素无明显相关(P＞0.05),与组织分化程度和淋巴结转移呈明显相关(P＜0.05).结论 PCNA在肝细胞癌组织中的表达高于正常肝组织和癌旁肝组织,并且与肿瘤的恶性程度密切相关.
目的 探討增殖細胞覈抗原(PCNA)在肝細胞肝癌的髮生髮展中的作用及作用機製.方法 採用逆轉錄-聚閤酶鏈反應(RT-PCR)和免疫組織化學法檢測PCNA在肝細胞癌、相應癌徬組織和正常肝組織中mRNA和蛋白質的錶達,正常肝組織、癌徬組織、肝細胞癌組織中的暘性錶達率分彆為20.0％、46.2％和85.7％；結閤患者的年齡、性彆、腫瘤大小、分化程度、有無淋巴結轉移等臨床病理學資料進行統計學分析,其錶達與組織分化程度和淋巴結轉移呈明顯相關(P＜0.05).結果 PCNA在正常肝組織、癌徬組織、肝細胞癌組織中的暘性錶達率分彆為20.0％、46.2％和85.7％,在肝細胞癌中的暘性錶達率顯著高于正常肝組織和癌徬肝組織(P＜0.05)；RT-PCR檢測結果也證實PCNA mRNA在肝細胞癌中的錶達明顯高于正常肝組織和癌徬肝組織(P＜0.05).PCNA的錶達與患者年齡、性彆、腫瘤大小等因素無明顯相關(P＞0.05),與組織分化程度和淋巴結轉移呈明顯相關(P＜0.05).結論 PCNA在肝細胞癌組織中的錶達高于正常肝組織和癌徬肝組織,併且與腫瘤的噁性程度密切相關.
목적 탐토증식세포핵항원(PCNA)재간세포간암적발생발전중적작용급작용궤제.방법 채용역전록-취합매련반응(RT-PCR)화면역조직화학법검측PCNA재간세포암、상응암방조직화정상간조직중mRNA화단백질적표체,정상간조직、암방조직、간세포암조직중적양성표체솔분별위20.0％、46.2％화85.7％；결합환자적년령、성별、종류대소、분화정도、유무림파결전이등림상병이학자료진행통계학분석,기표체여조직분화정도화림파결전이정명현상관(P＜0.05).결과 PCNA재정상간조직、암방조직、간세포암조직중적양성표체솔분별위20.0％、46.2％화85.7％,재간세포암중적양성표체솔현저고우정상간조직화암방간조직(P＜0.05)；RT-PCR검측결과야증실PCNA mRNA재간세포암중적표체명현고우정상간조직화암방간조직(P＜0.05).PCNA적표체여환자년령、성별、종류대소등인소무명현상관(P＞0.05),여조직분화정도화림파결전이정명현상관(P＜0.05).결론 PCNA재간세포암조직중적표체고우정상간조직화암방간조직,병차여종류적악성정도밀절상관.
Objective To study the mechanism of proliferating cell nuclear antigen (PCNA) in the pathogenesis and progression of hepatocellular carcinoma (HCC).Methods Reverse transcriptasepolymerase chain reaction (RT-PCR) and immunohistochemical technique were used to detect the mRNA and protein expression of PCNA in HCC tissues,adjacent tissues and normal liver tissues.The positive rates of protein expression of PCNA in HCC tissues,adjacent tissues and normal liver tissues were 87％,49％ and 20％.The expression levels of PCNA were analyzed combined with the clinicopathological factors including age,gender,tumor size,differentiation degree and lymph node metastasis.The expression of PCNA was correlated with differentiation degree and lymph node metastasis (P ＜ 0.05).Results The positive rates of protein expression of PCNA in HCC tissues,adjacent tissues and normal liver tissues were 87％,49％ and 20％.The mRNA and protein expression level of PCNA in HCC tissues was significantly higher than adjacent tissues and normal liver tissues (P ＜ 0.05).The expression of PCNA was correlated with differentiation degree and lymph node metastasis (P ＜0.05) and had no correlation with age,gender,tumor size (P ＞ 0.05).Conclusion The expression level of PCNA in HCC tissues was significantly higher than adjacent tissues and normal liver tissues which increase with the malignant degree of HCC.
