CAJ | 학술논문

目的观察胃癌患者癌组织及与其配对的正常胃黏膜组织中白细胞介素-8(IL-8)基因启动子甲基化及其mRNA表达,探讨IL-8基因甲基化与胃癌的关系.方法收集经病理确诊并行外科手术切除的胃癌组织及配对的正常胃黏膜组织各52例,用实时荧光定量聚合酶链反应(FQ-PCR)分别检测癌组织及正常组织中IL-8基因mRNA表达；用甲基化特异性PCR(MSP)检测上述两种组织中IL-8基因甲基化状态.结果胃癌组织中IL-8基因mRNA的表达量是正常组织中的1.94倍(P＜0.01).胃癌组织中IL-8基因甲基化率为32.7％,而正常组织中的甲基化率为73.1％(P＜0.01)；胃癌组织中IL-8基因mRNA表达及启动子的去甲基化与性别、年龄、肿瘤大小无明显相关(P＞0.05),而与肿瘤的分化程度、Borrmann分型、浸润程度及有无淋巴结转移均具有明显相关(P＜0.05)；去甲基化的胃癌组织中IL-8 mRNA表达是甲基化胃癌组织的2.13倍(P ＜0.001).结论 IL-8基因mRNA在胃癌组织中过表达；胃癌组织中IL-8基因启动子区呈低甲基化状态；IL-8基因启动子区去甲基化促进了其在胃癌组织中高表达.
목적 관찰위암환자암조직급여기배대적정상위점막조직중백세포개소-8(IL-8)기인계동자갑기화급기mRNA표체,탐토IL-8기인갑기화여위암적관계.방법 수집경병리학진병행외과수술절제적위암조직급배대적정상위점막조직각52례,용실시형광정량취합매련반응(FQ-PCR)분별검측암조직급정상조직중IL-8기인mRNA표체；용갑기화특이성PCR(MSP)검측상술량충조직중IL-8기인갑기화상태.결과 위암조직중IL-8기인mRNA적표체량시정상조직중적1.94배(P＜0.01).위암조직중IL-8기인갑기화솔위32.7％,이정상조직중적갑기화솔위73.1％(P＜0.01)；위암조직중IL-8기인mRNA표체급계동자적거갑기화여성별、년령、종류대소무명현상관(P＞0.05),이여종류적분화정도、Borrmann분형、침윤정도급유무림파결전이균구유명현상관(P＜0.05)；거갑기화적위암조직중IL-8 mRNA표체시갑기화위암조직적2.13배(P ＜0.001).결론 IL-8기인mRNA재위암조직중과표체；위암조직중IL-8기인계동자구정저갑기화상태；IL-8기인계동자구거갑기화촉진료기재위암조직중고표체.
Objective To investigate the promoter methylation status and mRNA expression of interleukin-8 (IL-8) in gastric cancer tissue and paired normal tissue,and to explore the relationship between IL-8 promoter methylation and gastric cancer.Methods The gastric cancer tissues (n =52) and paried normal tissues (n =52) confirmed pathologically and resected surgically were collected,and the mRNA expression of IL-8 was detected by using real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) and methylation status of the IL-8 gene by methylation-specific PCR (MSP).Results There was significant difference in IL-8 gene mRNA expression and methylation status between normal gastric mucosa and gastric cancer (P ＜ 0.01).The IL-8 gene mRNA expression and methylation status in gastric cancer were not significantly correlated with gender,age and tumor size (P ＞ 0.05),but significantly with degree of differentiation,Borrmann type,degree of infiltration and lymph node metastasis (P ＜ 0.05).There was also significant difference in the IL-8 mRNA expression between methylated gastric cancer tissue and unmethylated gastric cancer tissue (P ＜ 0.01).Conclusion The hypomethylation of IL-8 gene promoter promotes the over-expression of IL-8 mRNA in gastric cancer.