中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
8期
1741-1745
,共5页
齐晓薇%华东%游庆军%吴浩荣%金琳芳%周心一
齊曉薇%華東%遊慶軍%吳浩榮%金琳芳%週心一
제효미%화동%유경군%오호영%금림방%주심일
CXC趋化因子受体-5%CXCL13%结直肠癌%转移%预后
CXC趨化因子受體-5%CXCL13%結直腸癌%轉移%預後
CXC추화인자수체-5%CXCL13%결직장암%전이%예후
CXC chemokine receptor-5%CXCL13%Colorectal cancer%Metastasis%Prognosis
目的 观察CXC趋化因子受体-5(CXCR5)及其特异性配体CXCL13在结直肠癌组织中的表达,探讨其与临床病理特征、预后的关系.方法 用实时定量聚合酶链反应(Real-time PCR)法检测25对结直肠癌及13例结直肠腺瘤冰冻新鲜标本中CXCR5及CXCL13 mRNA的表达,应用免疫组织化学法(IHC)检测153例结直肠癌及相对应的癌旁组织、62例结直肠腺瘤标本中CXCR5及CXCL13蛋白的表达,分析其与临床病理特征、术后生存率的关系.结果 CXCR5及CXCL13mRNA及蛋白表达在结直肠癌组织中的表达率均高于癌旁组织及结直肠腺瘤组织(P均<0.05).CXCR5与CXCL13的mRNA及蛋白表达呈正相关(PCR:r =0.681,P<0.01;IHC:r =0.196,P<0.05).CXCR5-CXCL13蛋白表达与肿瘤的淋巴结转移、远处转移、肿瘤分期及复发相关;在有淋巴结转移、有远处转移、中晚期患者及出现复发的患者中阳性率都明显较高(P<0.05).此外,CXCL13阳性表达与结直肠癌的组织分化程度相关,分化越差组阳性率越高(P<0.05).CXCR5-CXCL13表达与其他临床病理特征无关(P>0.05).CXCR5及CXCL13阳性表达患者的5年复发率和5年生存率明显差于其阴性表达的患者(5年复发率:CXCR5:48.6%比14.8%,CXCL13:41.5%比22.7%;5年生存率:CXCR5:55.6%比91.4%,CXCL13:61.5%比84.1%)(P<0.05);CXCR5及CXCL13阳性表达患者的中位复发时间和中位生存时间明显短于其阴性表达的患者[中位复发时间:CXCR5 (13.0±1.3)个月比(45.0±7.8)个月,CXCL13(13.0±1.3)个月比(29.0±11.2)个月;中位生存时间:CXCR5(17.0±1.1)个月比(55.0±14.4)个月,CXCL13(17.0±1.9)个月比(25.0±11.2)个月](P<0.05).结论 CXCR5及CXCL13在结直肠癌的发生、发展和转移、复发中可能起着重要的作用,可作为预测结直肠癌转移和复发的有价值指标.
目的 觀察CXC趨化因子受體-5(CXCR5)及其特異性配體CXCL13在結直腸癌組織中的錶達,探討其與臨床病理特徵、預後的關繫.方法 用實時定量聚閤酶鏈反應(Real-time PCR)法檢測25對結直腸癌及13例結直腸腺瘤冰凍新鮮標本中CXCR5及CXCL13 mRNA的錶達,應用免疫組織化學法(IHC)檢測153例結直腸癌及相對應的癌徬組織、62例結直腸腺瘤標本中CXCR5及CXCL13蛋白的錶達,分析其與臨床病理特徵、術後生存率的關繫.結果 CXCR5及CXCL13mRNA及蛋白錶達在結直腸癌組織中的錶達率均高于癌徬組織及結直腸腺瘤組織(P均<0.05).CXCR5與CXCL13的mRNA及蛋白錶達呈正相關(PCR:r =0.681,P<0.01;IHC:r =0.196,P<0.05).CXCR5-CXCL13蛋白錶達與腫瘤的淋巴結轉移、遠處轉移、腫瘤分期及複髮相關;在有淋巴結轉移、有遠處轉移、中晚期患者及齣現複髮的患者中暘性率都明顯較高(P<0.05).此外,CXCL13暘性錶達與結直腸癌的組織分化程度相關,分化越差組暘性率越高(P<0.05).CXCR5-CXCL13錶達與其他臨床病理特徵無關(P>0.05).CXCR5及CXCL13暘性錶達患者的5年複髮率和5年生存率明顯差于其陰性錶達的患者(5年複髮率:CXCR5:48.6%比14.8%,CXCL13:41.5%比22.7%;5年生存率:CXCR5:55.6%比91.4%,CXCL13:61.5%比84.1%)(P<0.05);CXCR5及CXCL13暘性錶達患者的中位複髮時間和中位生存時間明顯短于其陰性錶達的患者[中位複髮時間:CXCR5 (13.0±1.3)箇月比(45.0±7.8)箇月,CXCL13(13.0±1.3)箇月比(29.0±11.2)箇月;中位生存時間:CXCR5(17.0±1.1)箇月比(55.0±14.4)箇月,CXCL13(17.0±1.9)箇月比(25.0±11.2)箇月](P<0.05).結論 CXCR5及CXCL13在結直腸癌的髮生、髮展和轉移、複髮中可能起著重要的作用,可作為預測結直腸癌轉移和複髮的有價值指標.
목적 관찰CXC추화인자수체-5(CXCR5)급기특이성배체CXCL13재결직장암조직중적표체,탐토기여림상병리특정、예후적관계.방법 용실시정량취합매련반응(Real-time PCR)법검측25대결직장암급13례결직장선류빙동신선표본중CXCR5급CXCL13 mRNA적표체,응용면역조직화학법(IHC)검측153례결직장암급상대응적암방조직、62례결직장선류표본중CXCR5급CXCL13단백적표체,분석기여림상병리특정、술후생존솔적관계.결과 CXCR5급CXCL13mRNA급단백표체재결직장암조직중적표체솔균고우암방조직급결직장선류조직(P균<0.05).CXCR5여CXCL13적mRNA급단백표체정정상관(PCR:r =0.681,P<0.01;IHC:r =0.196,P<0.05).CXCR5-CXCL13단백표체여종류적림파결전이、원처전이、종류분기급복발상관;재유림파결전이、유원처전이、중만기환자급출현복발적환자중양성솔도명현교고(P<0.05).차외,CXCL13양성표체여결직장암적조직분화정도상관,분화월차조양성솔월고(P<0.05).CXCR5-CXCL13표체여기타림상병리특정무관(P>0.05).CXCR5급CXCL13양성표체환자적5년복발솔화5년생존솔명현차우기음성표체적환자(5년복발솔:CXCR5:48.6%비14.8%,CXCL13:41.5%비22.7%;5년생존솔:CXCR5:55.6%비91.4%,CXCL13:61.5%비84.1%)(P<0.05);CXCR5급CXCL13양성표체환자적중위복발시간화중위생존시간명현단우기음성표체적환자[중위복발시간:CXCR5 (13.0±1.3)개월비(45.0±7.8)개월,CXCL13(13.0±1.3)개월비(29.0±11.2)개월;중위생존시간:CXCR5(17.0±1.1)개월비(55.0±14.4)개월,CXCL13(17.0±1.9)개월비(25.0±11.2)개월](P<0.05).결론 CXCR5급CXCL13재결직장암적발생、발전화전이、복발중가능기착중요적작용,가작위예측결직장암전이화복발적유개치지표.
Objective To investigate the correlation between CXC chemokine receptor-5 (CXCR5)-CXCL13 expression and clinicopathological features in colorectal carcinoma.Methods The real-time transcription polymerase chain reaction (Real-time PCR) technique was used to examine mRNA expression of CXCR5 and CXCL13 in 25 paired specimens of colorectal cancer and tissues adjacent carcinoma and 13 samples of colorecal adenoma.The immunohistochemistry staining method was used to detect the protein expression of CXCR5 and CXCL13 in 153 paired specimens of colorectal cancer and tissues adjacent carcinoma and 62 samples of colorecal adenoma.Results The mRNA and protein levels of CXCR5-CXCL13 in colorectal cancer were up-regulated than tissues adjacent carcinoma and adenoma (P < 0.05).The mRNA and protein levels of CXCR5-CXCL13 in colorectal cancer correlated positively (PCR:r =0.681,P < 0.01 ; IHC:r =0.196,P < 0.05).The positive stain of CXCR5-CXCLI3 correlated with lymph node involved,metastasis,higher TNM stage and relapse (P < 0.05).Additionally,CXCL13 positive staining correlated with poor differentiation (P < 0.05).By Kaplan-Merie' s analysis,CXCR5-CXCL13 positive staining favored poor prognosis parameters,such as 5-year relapse rate (CXCR5:48.6% vs.14.8%,CXCL13:41.5% vs.22.7%),5-year survival rate (CXCR5:55.6% vs.91.4%,CXCL13:61.5% vs.84.1%),the median disease free time [CXCR5:(13.0 ± 1.3) months vs.(45.0 ± 7.8) months,CXCL13:(13.0 ± 1.3) months vs.(29.0 ± 11.2) months] and the median survival time [CXCR5:(17.0 ± 1.1) months vs.(55.0 ± 14.4) months,CXCL13:(17.0 ± 1.9) months vs.(25.0 ± 11.2)months] (P <0.05).Conclusion CXCR5 and CXCL13 may play a crucial role in carcinogenesis,development,metastasis and relapse of colon cancer.It can be used as prognostic markers of colon cancer in clinical practice.