CAJ | 학술논문

目的观察热休克蛋白70羧基末端相互作用蛋白(CHIP)小干扰RNA(siRNA)对人脑胶质瘤细胞周期及增殖的影响.方法运用Control siRNA、CHIP siRNA分别转染原代培养人脑胶质瘤细胞,Western blot检测CHIP蛋白表达,流式细胞仪检测CHIP siRNA对原代培养人脑胶质瘤细胞周期的影响,细胞计数试剂盒(CCK-8)细胞增殖实验检测CHIP siRNA对原代培养人脑胶质瘤细胞增殖的影响.结果与Control siRNA组比较CHIP siRNA组原代培养人脑胶质瘤细胞CHIP蛋白表达量降低53.3％,CHIP siRNA组原代培养人脑胶质瘤细胞增殖细胞数增加,并且G1期细胞数所占比例减少14.1％,S期细胞数所占比例增加13.7％ (P ＜0.05).结论 CHIP siRNA靶向干扰了CHIP的表达,CHIP作为肿瘤抑癌因子抑制脑胶质瘤细胞的增殖,并能使细胞阻滞在G1期.
목적 관찰열휴극단백70최기말단상호작용단백(CHIP)소간우RNA(siRNA)대인뇌효질류세포주기급증식적영향.방법 운용Control siRNA、CHIP siRNA분별전염원대배양인뇌효질류세포,Western blot검측CHIP단백표체,류식세포의검측CHIP siRNA대원대배양인뇌효질류세포주기적영향,세포계수시제합(CCK-8)세포증식실험검측CHIP siRNA대원대배양인뇌효질류세포증식적영향.결과 여Control siRNA조비교CHIP siRNA조원대배양인뇌효질류세포CHIP단백표체량강저53.3％,CHIP siRNA조원대배양인뇌효질류세포증식세포수증가,병차G1기세포수소점비례감소14.1％,S기세포수소점비례증가13.7％ (P ＜0.05).결론 CHIP siRNA파향간우료CHIP적표체,CHIP작위종류억암인자억제뇌효질류세포적증식,병능사세포조체재G1기.
Objective To investigate the effect of carboxyl terminus of Hsc70-interacting protein (CHIP) small interfering RNA (siRNA) on cell cycle and proliferation of human brain glioma cells.Methods Control siRNA and CHIP siRNA were used respectively to transfect primary cultured human brain glioma cells.The expression levels of CHIP in primary cultured human brain glioma cells were detected after CHIP knockdown by using Western blotting.The influence of CHIP knockdown on the cell cycle of primary cultured human brain glioma cells was measured by flow cytometry.The influence of CHIP knockdown on the proliferation of primary cultured human brain glioma cells was tested by using cell counting kit-8 (CCK-8)assay.Results As compared with control siRNA group,the expression of CHIP was decreased by 53.3％ in CHIP siRNA group.CHIP knockdown could drastically increase the ability of proliferation in primary cultured human brain glioma cells,increase the S phase ratio of cell cycle by 13.7％ and decrease the G1 phase ratio of cell cycle by 14.1％ (both P ＜ 0.05).Conclusion CHIP-targeted siRNA interferes with the expression levels of CHIP.CHIP acts as a tumor suppressor that suppresses the proliferation of glioma cells and arrests the cells in G1 phase.