CAJ | 학술논문

目的观察大麻受体激动剂花生四烯酸乙醇胺(AEA)人U251胶质瘤细胞黏附、趋化运动及侵袭生物学行为的抑制作用.方法以5μmol/L AEA 12 h和24 h的预处理U251细胞,应用噻唑蓝(MTT)比色法测定U251细胞黏附作用的影响,应用Transwell小室实验检测U251细胞趋化运动和侵袭能力的影响.结果 5μmol/L AEA具有时间依赖性抑制U251细胞黏附、趋化运动及侵袭生物学行为的作用；预处理12 h,U251细胞黏附、趋化运动能力和侵袭能力分别降低45.14％、45.24％和41.86％；预处理24 h后,3种能力分别下降66.53％、81.08％和83.85％.结论 AEA具有对人U251胶质瘤细胞黏附、趋化运动及侵袭生物学行为的抑制作用,且具有时间相关性.
목적 관찰대마수체격동제화생사희산을순알(AEA)인U251효질류세포점부、추화운동급침습생물학행위적억제작용.방법 이5μmol/L AEA 12 h화24 h적예처리U251세포,응용새서람(MTT)비색법측정U251세포점부작용적영향,응용Transwell소실실험검측U251세포추화운동화침습능력적영향.결과 5μmol/L AEA구유시간의뢰성억제U251세포점부、추화운동급침습생물학행위적작용；예처리12 h,U251세포점부、추화운동능력화침습능력분별강저45.14％、45.24％화41.86％；예처리24 h후,3충능력분별하강66.53％、81.08％화83.85％.결론 AEA구유대인U251효질류세포점부、추화운동급침습생물학행위적억제작용,차구유시간상관성.
Objective To investigate the effects of the cell adhesion,chemotactic motility and invasion in U251 glioma cell by anandamide (AEA) treatment.Methods Human U251 glioma cell line were cultured with DMEM's medium in vitro.Cultured cells were divided into control group,and 5 μmol/L AEA groups for 12 h and 24 h.The cell adhesion rate of U251 cell was investigated by methyl thiazol tetrazolium (MTT) assay at different times of AEA treatment.The abilities of chemotactic motility and invasion were detected by Transwell.Results MTF showed that AEA inhibited cell adhesion and the rate increased with increasing treatment time of 5 μmol/L AEA.The inhibitive rates were respectively 45.14％ for 12 h (P ＜ 0.05) and 66.53％ for 24 h (P ＜ 0.01).The abilities of chemotactic motility and invasion also were depressed respectively 45.24％ and 41.86％ for 12 h (P ＜ 0.05).Those rates were 81.08％ and 83.85％ for 24h (P ＜0.01).Conclusion AEA could inhibit the abilities of cell adhesion,chemotactic motility and invasion on human U251 glioma cell line,and this effect has the time dependence.