中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
9期
1828-1830
,共3页
张猛%何毅%闫大为%张秋生%刘玉飞%李维平
張猛%何毅%閆大為%張鞦生%劉玉飛%李維平
장맹%하의%염대위%장추생%류옥비%리유평
垂体瘤%Wnt4%信号转导通路%模型,动物
垂體瘤%Wnt4%信號轉導通路%模型,動物
수체류%Wnt4%신호전도통로%모형,동물
Pituitary adenoma%Wnt4%Signaling transduction pathway%Model,animal
目的 检测己烯雌酚诱导的大鼠垂体瘤动物模型成瘤过程中垂体组织中Wnt4表达的动态变化,探讨Wnt4信号转导通路在垂体瘤发生发展过程中的作用及其机制.方法 将60只标准Wistar-Furth大鼠随机分成己烯雌酚注射组(30只)、灭菌花生油注射组及假处理组(各15只),观察处理后大鼠外观、体质量及行为学改变,并分别于4、8及12周处死大鼠,观察成瘤情况、垂体外观及重量变化,应用苏木素-伊红(HE)染色及免疫组织化学染色的方法鉴别肿瘤类型及检测Wnt4表达.结果 3个处死时间点实验组成瘤率分别为30％、90％、100％,垂体组织Wnt4阳性表达积分分别为5.80±3.32、8.40±2.22、9.70±1.64;对照组成瘤率均为0,垂体组织Wnt4阳性表达积分分别为0.80±0.83、1.40±1.14、1.20±0.83.无论成瘤与否,Wnt4阳性表达积分与对照组比较差异均有统计学意义(P＜0.05),随着建模时间延长,实验组垂体组织中Wnt4的表达呈进行性增高.结论 雌激素诱导大鼠垂体泌乳素腺瘤动物模型成瘤效果确切稳定,可作为治疗研究模型,Wnt4信号通路在垂体瘤的发病与进展中起重要作用.
目的 檢測己烯雌酚誘導的大鼠垂體瘤動物模型成瘤過程中垂體組織中Wnt4錶達的動態變化,探討Wnt4信號轉導通路在垂體瘤髮生髮展過程中的作用及其機製.方法 將60隻標準Wistar-Furth大鼠隨機分成己烯雌酚註射組(30隻)、滅菌花生油註射組及假處理組(各15隻),觀察處理後大鼠外觀、體質量及行為學改變,併分彆于4、8及12週處死大鼠,觀察成瘤情況、垂體外觀及重量變化,應用囌木素-伊紅(HE)染色及免疫組織化學染色的方法鑒彆腫瘤類型及檢測Wnt4錶達.結果 3箇處死時間點實驗組成瘤率分彆為30％、90％、100％,垂體組織Wnt4暘性錶達積分分彆為5.80±3.32、8.40±2.22、9.70±1.64;對照組成瘤率均為0,垂體組織Wnt4暘性錶達積分分彆為0.80±0.83、1.40±1.14、1.20±0.83.無論成瘤與否,Wnt4暘性錶達積分與對照組比較差異均有統計學意義(P＜0.05),隨著建模時間延長,實驗組垂體組織中Wnt4的錶達呈進行性增高.結論 雌激素誘導大鼠垂體泌乳素腺瘤動物模型成瘤效果確切穩定,可作為治療研究模型,Wnt4信號通路在垂體瘤的髮病與進展中起重要作用.
목적 검측기희자분유도적대서수체류동물모형성류과정중수체조직중Wnt4표체적동태변화,탐토Wnt4신호전도통로재수체류발생발전과정중적작용급기궤제.방법 장60지표준Wistar-Furth대서수궤분성기희자분주사조(30지)、멸균화생유주사조급가처리조(각15지),관찰처리후대서외관、체질량급행위학개변,병분별우4、8급12주처사대서,관찰성류정황、수체외관급중량변화,응용소목소-이홍(HE)염색급면역조직화학염색적방법감별종류류형급검측Wnt4표체.결과 3개처사시간점실험조성류솔분별위30％、90％、100％,수체조직Wnt4양성표체적분분별위5.80±3.32、8.40±2.22、9.70±1.64;대조조성류솔균위0,수체조직Wnt4양성표체적분분별위0.80±0.83、1.40±1.14、1.20±0.83.무론성류여부,Wnt4양성표체적분여대조조비교차이균유통계학의의(P＜0.05),수착건모시간연장,실험조수체조직중Wnt4적표체정진행성증고.결론 자격소유도대서수체비유소선류동물모형성류효과학절은정,가작위치료연구모형,Wnt4신호통로재수체류적발병여진전중기중요작용.
Objective To observe the dynamic changes of Wnt4 expression in pituitary tissue fiom rats with pituitary adenoma models induced by diethylstibestrol (Des),and probe into the possible mechanism of Wnt4 signal transduction pathway in tumorigenesis and development of pituitary adenomas.Methods Sixty Wistar rats were randomly divided into DES-treated group (Des),arachis (o)il-treated control group (AO) and feign-treated control group (FT).Behavior changes and body weight were recorded.Ten rats in DES-treated group and 5 in each control group were sacrificed after 4,8 and 12 weeks,respectively.The weight,histological and morphological changes of the pituitary gland,serum prolactin (PRL) level,and expression of Wnt4 were detected.Results Tumor formation rate was 30％,90％ and 100％,and the Wnt4 positive scores was 5.80 ±3.32,8.40 ±2.22 and 9.70 ± 1.64 at 4th,8th and 12th week in Des group,respectively.The Wnt4 positive scores in control group were 0.80 ± 0.83,1.40 ± 1.14 and 1.20 ± 0.83 at 4th,8th and 12th week,respectively.Wnt4 expression was significantly higher in Des group than in other groups (P ＜ 0.05),and had acending trend along with the treating time of Des.Conclusion Pituitary tumor model induced by Des in Wistar rats was a stable and convenient pituitary tumor model.Wnt4 signal transduction pathway may play an important role in tumorigenesis and development of pituitary adenomas.
