CAJ | 학술논문

目的观察依托咪酯对成年大鼠视神经损伤后功能恢复的作用.方法应用大鼠视神经挤压伤模型,并将大鼠随机分为依托咪酯治疗组、治疗对照组和空白对照组,分析各组荧光金逆行标记的视网膜神经节细胞(RGCs)、视神经闪光视觉诱发电位(FVEP)变化、RGCs再生轴突和视网膜蛋白激酶C(PKC)活性变化.结果视神经损伤后7、14d,依托咪酯治疗组存活RGCs密度[分别为(1825±96)/mm2和(924±67)/mm2],均显著高于空白对照组[分别为(1420±88)/mm2和(625±42)/mm2,P＜0.05]和治疗对照组[分别为(1502±85)个/mm2和(698±50)个/mm2；P＜0.05],而后两组差异无统计学意义(P>0.05)；视神经损伤后21 d,3组差异均无统计学意义(P＞0.05).视神经损伤后28 d,依托咪酯治疗组FVEP P100波潜伏期[(68.59 ±8.75) ms]比空白对照组和治疗对照组[分别为(118.37±12.63)、(112.51±10.78) ms]明显缩短(P＜0.05),波幅[(6.85 ±2.47) mV]比空白对照组和治疗对照组[分别为(3.45±1.24)、(3.87±1.59)mV]显著增高(P＜0.05).而且,依托咪酯治疗组RGCs再生轴突数量均明显高于空白对照组和治疗对照组(P＜0.05),但其视网膜PKC活性却显著降低(P＜0.05).结论依托咪酯对成年大鼠视神经挤压伤后功能修复具有促进作用.
목적 관찰의탁미지대성년대서시신경손상후공능회복적작용.방법 응용대서시신경제압상모형,병장대서수궤분위의탁미지치료조、치료대조조화공백대조조,분석각조형광금역행표기적시망막신경절세포(RGCs)、시신경섬광시각유발전위(FVEP)변화、RGCs재생축돌화시망막단백격매C(PKC)활성변화.결과 시신경손상후7、14d,의탁미지치료조존활RGCs밀도[분별위(1825±96)/mm2화(924±67)/mm2],균현저고우공백대조조[분별위(1420±88)/mm2화(625±42)/mm2,P＜0.05]화치료대조조[분별위(1502±85)개/mm2화(698±50)개/mm2；P＜0.05],이후량조차이무통계학의의(P>0.05)；시신경손상후21 d,3조차이균무통계학의의(P＞0.05).시신경손상후28 d,의탁미지치료조FVEP P100파잠복기[(68.59 ±8.75) ms]비공백대조조화치료대조조[분별위(118.37±12.63)、(112.51±10.78) ms]명현축단(P＜0.05),파폭[(6.85 ±2.47) mV]비공백대조조화치료대조조[분별위(3.45±1.24)、(3.87±1.59)mV]현저증고(P＜0.05).이차,의탁미지치료조RGCs재생축돌수량균명현고우공백대조조화치료대조조(P＜0.05),단기시망막PKC활성각현저강저(P＜0.05).결론 의탁미지대성년대서시신경제압상후공능수복구유촉진작용.
Objective To investigate the effect of etomidate on the functional recovery after optic nerve crush in adult rats.Methods The left optic nerves of adult SD rats were crushed at 2 mm away from the optic disc.The rats were then randomly divided into three groups:etomidate-treated group,vehicle group and control group.The number of surviving retinal ganglion cells (RGCs) labeled retrogradely with fluorogold,the flash visual evoked potential (FVEP),the regenerating axons of RGCs,and the activity of protein kinase C (PKC) were analyzed in all groups.Results Seven and fourteen days after cmsh,the densities of surviving RGCs were significantly higher in etomidate-treated group [(1825 ± 96) cells/mm2 and (924 ± 67)cells/mm2,respectively] than in vehicle group [(1502± 85) cells/mm2 and (698 ±50) cells/mm2,respectively,P ＜ 0.05] or control group [(1420 ± 88) cells/mm2 and (625 ± 42) cells/mm2,respectively,P ＜ 0.05],but there were no significant differences between vehicle and control groups (P ＞ 0.05).However,21 days after crush,there were no significant differences among the three groups (P ＞ 0.05).Twenty-eight days after crush,the latency [(68.59 ± 8.75) ms] was significantly decreased and the amplitude [(6.85 ± 2.47) mV] significantly increased in etomidate-treated group compared to vehicle group [(112.51 ± 10.78) ms and (3.87 ± 1.59) mV,respectively,P ＜ 0.05] and control group [(118.37 ±12.63) ms and (3.45 ± 1.24) mV,respectively,P ＜ 0.05].Moreover,the number of regenerating axons of RGCs was significantly increased and the activity of PKC significantly decreased in etomidate-treated group compared to vehicle group (P ＜ 0.05) or control group (P ＜ 0.05).Conclusion Etomidate can promote functional recovery after optic nerve crush in adult rats.