CAJ | 학술논문

目的观察大鼠颅脑液压冲击伤后水肿脑组织水通道蛋白4(AQP4)蛋白及其mRNA的表达变化.方法成年雄性SD大鼠64只,应用Dixon法制作颅脑损伤模型,术后6、12、24 h、3、7d用干湿重法、免疫组织化学法、Western blot以及逆转录-聚合酶链反应(RT-PCR)分别测定水肿脑组织含水量、AQP4蛋白及其mRNA的表达.结果自伤后6h,脑组织含水量开始增加(79.11±1.28)％,24 h达高峰[(82.74±1.10)％],持续至3 d[(82.04±1.69)％],然后开始下降.组织学观察印证了脑组织的水肿趋势.AQP4于伤后6h开始增加[积分吸光度(IA)值为471.40±45.19],24 h达高峰(IA值为944.20±101.84),持续至3d后下降(IA值为920.60±194.22),7d仍高于对照组；而AQP4 mRNA表达变化趋势与其蛋白含量变化一致.线性相关分析可见AQP4蛋白及其mRNA表达与脑含水量呈正相关(rp=0.981;rm =0.973,P＜0.05).结论 AQP4表达上调在液压冲击伤性脑水肿演变中起重要作用.
목적 관찰대서로뇌액압충격상후수종뇌조직수통도단백4(AQP4)단백급기mRNA적표체변화.방법 성년웅성SD대서64지,응용Dixon법제작로뇌손상모형,술후6、12、24 h、3、7d용간습중법、면역조직화학법、Western blot이급역전록-취합매련반응(RT-PCR)분별측정수종뇌조직함수량、AQP4단백급기mRNA적표체.결과 자상후6h,뇌조직함수량개시증가(79.11±1.28)％,24 h체고봉[(82.74±1.10)％],지속지3 d[(82.04±1.69)％],연후개시하강.조직학관찰인증료뇌조직적수종추세.AQP4우상후6h개시증가[적분흡광도(IA)치위471.40±45.19],24 h체고봉(IA치위944.20±101.84),지속지3d후하강(IA치위920.60±194.22),7d잉고우대조조；이AQP4 mRNA표체변화추세여기단백함량변화일치.선성상관분석가견AQP4단백급기mRNA표체여뇌함수량정정상관(rp=0.981;rm =0.973,P＜0.05).결론 AQP4표체상조재액압충격상성뇌수종연변중기중요작용.
Objective To investigate the expression of apuaporin 4 (AQP4) protein and mRNA in cerebral edema following fluid percussion injury in rats.Methods The experimental models were established in 64 rats according to Dixon's method.The water content of injured brain and the expression of AQP4 protein and mRNA were measured by using dry-wet measure,immunohistochemistry,Western blotting and reverse transcriptase-polymerase chain reaction (RT-PCR) at 1st h,6th h,12th h,24th h,3rd day and 7th day after operation respectively.Results As compared with control group,the water content was increased in model group from 6 h [(79.11 ± 1.28) ％],reached the peak at 24 h [(82.74 ±1.10)％],lasted to 3rd day [(82.04 ± 1.69)％],and then began to drop; the AQP4 expression was increased from 6 h (IA 471.40 ±45.19),reached the maximum at 24 h (IA 944.20 ± 101.84),lasted to 3rd day (IA 920.60 ± 194.22) and began to drop.Until 7th day,the AQP4 immunoreactive expression was still higher in model group than in control group.The linear regression analysis indicated that the expression of AQP4 protein and mRNA in edematous brain had a positive correlation with change of brain water content (rp,=0.981 ;rm =0.973,P ＜ 0.05).Conclusion The AQP4 expression upregulation plays an important role in cerebral edema following fluid percussion injury in rats and inhibition of AQP4 upregulation may become the new strategy for treatment of traumatic brain edema.