CAJ | 학술논문

目的通过磁共振成像(MRI)、超微结构评估不同剂量伽玛射线对大鼠海马组织的影响,并分析照射前后大鼠基因表达谱的变化.方法分别用照射剂量为20、40、60和l00 Gy的伽玛射线对正常大鼠海马进行照射,于照射后3、5和7个月进行MRI检查,于照射后3个月进行电镜检查,同时用基因芯片技术检测照射剂量为25 Gy时基因表达谱的变化,并对差异基因进行功能分类.结果照射剂量为20 Gy时,大鼠MRI和电镜检查未见明显变化,随着照射剂量的增加和时间的延长,逐渐出现放射性损伤.MRI表现为混杂信号,电镜显示神经元固缩、内皮细胞破坏等变化.差异基因涉及生物学进程、分子组成等功能.结论伽玛射线对大鼠海马组织的放射生物学作用与照射剂量有关.基因芯片技术可检测伽玛刀照射前后基因表达谱变化,并对差异基因进行功能分类.
목적 통과자공진성상(MRI)、초미결구평고불동제량가마사선대대서해마조직적영향,병분석조사전후대서기인표체보적변화.방법 분별용조사제량위20、40、60화l00 Gy적가마사선대정상대서해마진행조사,우조사후3、5화7개월진행MRI검사,우조사후3개월진행전경검사,동시용기인심편기술검측조사제량위25 Gy시기인표체보적변화,병대차이기인진행공능분류.결과 조사제량위20 Gy시,대서MRI화전경검사미견명현변화,수착조사제량적증가화시간적연장,축점출현방사성손상.MRI표현위혼잡신호,전경현시신경원고축、내피세포파배등변화.차이기인섭급생물학진정、분자조성등공능.결론 가마사선대대서해마조직적방사생물학작용여조사제량유관.기인심편기술가검측가마도조사전후기인표체보변화,병대차이기인진행공능분류.
Objective To investigate the effect of Leksell gamma knife irradiation on normal rat hippocampus through magnetic resonance imaging (MRI) and ultra-structure and analyze the gene expression profile of the rat hippocampus before and after the irradiation of the Leksell gamma knife.Methods The normal rats were irradiated with doses of 20,40,60 and 100 Gy,and 4-mm isocenters were used to cover the right hippocampus.MRI was performed at 3rd,5th and 7th month after radiosurgery with gamma knife.Rat was killed and the brain was removed to receive ultra-structural examination at 3rd month after irradiation under electron microscope.Meanwhile,we used gene chip to investigate the expression profile of genes and their function in the rats 3 months after irradiation with 25 Gy and 8-nm isocenters.Results After irradiation with 20 Gy,there were no obvious changes on MRI and ultra-structural examinations of the rat hippocampus.The damages of rat brain were aggravated with the increase of irradiation dose and the extension of duration.Mixed signals occurred in MRI,and electron microscopy showed neuronal pyknosis,endothelial cell damage,etc.The genes differentially expressed were categorized into different functions incIuding molecular function,biological process,cellular component,etc.Conclusion The effect of gamma knife on brain tissue appeared gradually with the extension of time and the increase of irradiation dose.The microarray analysis can screen the genes differentially expressed before and after irradiation,and classify the differentially expressed genes functionally.