CAJ | 학술논문

目的观察不同时程的慢性应激对大鼠抑郁行为和海马神经再生的影响.方法随机将大鼠分为4组:(A)正常对照组(12只)；(B)l周慢性应激组(12只)；(C)2周慢性应激组(12只)；(D)3周慢性应激组(12只),每组大鼠在应激前后用糖水偏好测验和旷场测验评估行为,采用免疫组织化学方法检测大鼠海马齿状回的神经再生数量.结果 (1)行为学结果:大鼠旷场内10 min的总行程、内围场地的行程、直立次数,3组应激组大鼠均明显小于对照组(P＜0.05),3组应激组大鼠之间差异无统计学意义(P＞0.05)；大鼠总液体消耗量、糖水消耗量、糖水偏好率,3组应激组大鼠均明显小于对照组(P＜0.05),3组应激组大鼠之间差异无统计学意义(P＞0.05).(2)各组大鼠海马齿状回内均可以见到溴脱氧尿嘧啶核苷(BrdU)标记阳性的细胞.l周应激组大鼠的海马齿状回BrdU阳性细胞数[(22.19±6.08)个]低于对照组的海马齿状回BrdU阳性细胞数[(31.75±8.48)个,P＜0.05],3周应激组大鼠的海马齿状回BrdU阳性细胞数[(19.57±5.28)个]也明显低于对照组的海马齿状回BrdU阳性细胞数[(31.75±8.48)个,P＜0.01],1周应激组与3周应激组大鼠的海马齿状回BrdU阳性细胞数之间比较差异无统计学意义(P＞0.05).2周应激组大鼠的海马齿状回BrdU阳性细胞数[(29.78±6.78)个]与对照组的海马齿状回BrdU阳性细胞数[(31.75 ±8.48)个]差异无统计学意义(P＞0.05).结论不同时程的慢性应激均可致抑郁行为,但对海马齿状回神经再生有不同的影响.
목적 관찰불동시정적만성응격대대서억욱행위화해마신경재생적영향.방법 수궤장대서분위4조:(A)정상대조조(12지)；(B)l주만성응격조(12지)；(C)2주만성응격조(12지)；(D)3주만성응격조(12지),매조대서재응격전후용당수편호측험화광장측험평고행위,채용면역조직화학방법검측대서해마치상회적신경재생수량.결과 (1)행위학결과:대서광장내10 min적총행정、내위장지적행정、직립차수,3조응격조대서균명현소우대조조(P＜0.05),3조응격조대서지간차이무통계학의의(P＞0.05)；대서총액체소모량、당수소모량、당수편호솔,3조응격조대서균명현소우대조조(P＜0.05),3조응격조대서지간차이무통계학의의(P＞0.05).(2)각조대서해마치상회내균가이견도추탈양뇨밀정핵감(BrdU)표기양성적세포.l주응격조대서적해마치상회BrdU양성세포수[(22.19±6.08)개]저우대조조적해마치상회BrdU양성세포수[(31.75±8.48)개,P＜0.05],3주응격조대서적해마치상회BrdU양성세포수[(19.57±5.28)개]야명현저우대조조적해마치상회BrdU양성세포수[(31.75±8.48)개,P＜0.01],1주응격조여3주응격조대서적해마치상회BrdU양성세포수지간비교차이무통계학의의(P＞0.05).2주응격조대서적해마치상회BrdU양성세포수[(29.78±6.78)개]여대조조적해마치상회BrdU양성세포수[(31.75 ±8.48)개]차이무통계학의의(P＞0.05).결론 불동시정적만성응격균가치억욱행위,단대해마치상회신경재생유불동적영향.
Objective To explore the effect of different durations of chronic unpredictable mild stress (CUMS) on behaiviors and neurogenesis of bippocampal dentate gyrus in adult rats.Methods (1)Animals were divided into four groups:control group,rats without stress; M1 group,rats subject to CUMS for 1 week; M2 group,rats subject to CUMS for 2 weeks; and M5 group,rats subject to CUMS for 3 weeks.(2) Rats'depression-like behaviors were observed in opening field test and 1％ sucrose preference test was done before and after the experiment of each group.(3) After bahavior test,rats were sacrificed and the hippocampi were isolated.Neurogenesis in hippocampal dentate gyrus was detected by using immunohistochemistry.Results (1) As compared with control group,total diatance (cm) in l0 min/center distance (cm) in 10 min/rearing counts were decreased in MI group (P ＜ 0.05),M2 group (P ＜ 0.05),and M3 group (P ＜0.05).There was no significant difference in the total distance in 10 min/center distance (cm) in 10 min/rearing counts among the M1,M2 and M3 groups (P ＞ 0.05).As compared with control group,levels of total fluid consumption/sucrose consumption/sucrose preference consumption were decreased in MI group (P ＜ 0.05),M2 group (P ＜ 0.05),and M3 group (P ＜ 0.05).There was no significant difference in levels of total fluid consumption among the M1,M2 and M3 groups (P ＞ 0.05) ; (2)As compared with control group (31.75 ± 8.48),BrdU-labeled cells in the dentate gyrus were decreased in M1 group (22.19 ±6.08) (P＜0.05) and M3 group (19.57 ±5.28) (P＜0.05),but there was no significant difference in the levels of BrdU-labeled cells in the dentate gyrus between M1 group and M3 group (P ＞ 0.05).There was no significant difference in the levels of BrdU-labeled cells in the dentate gyrus between M2 group (29.78 ± 6.78) and control group (P ＞ 0.05).Conclusion Different durations of CUMS could lead to the depressive behaviors,but have different influences on the neurogenesis of hippocampal dentate gyrus in adult rats.