CAJ | 학술논문

目的制作大鼠颅脑液压冲击伤后早期癫痫发作模型,观察海马的胶质纤维酸性蛋白(GFAP)和海马苔状纤维变化.方法将大鼠随机分组,实验组按照不同的打击力度制作颅脑液压冲击伤模型,测定伤后7d大鼠海马胶质纤维酸性蛋白(GFAP)的表达,行Timm染色,镜下观察.结果重度冲击伤组大鼠均出现了早期癫痫发作,GFAP平均积分吸光度(A)值:CA3区为38.87±1.24,齿状回为42.35±1.40,苔藓纤维Timm染色颗粒的平均积分A值:1.38 ±0.10,与对照组比较,差异有统计学意义(P＜0.05).结论海马区域GFAP表达和苔状纤维出芽增生在伤后早期癫痫发作大鼠明显增加,与癫痫发作敏感性密切相关.
목적 제작대서로뇌액압충격상후조기전간발작모형,관찰해마적효질섬유산성단백(GFAP)화해마태상섬유변화.방법 장대서수궤분조,실험조안조불동적타격력도제작로뇌액압충격상모형,측정상후7d대서해마효질섬유산성단백(GFAP)적표체,행Timm염색,경하관찰.결과 중도충격상조대서균출현료조기전간발작,GFAP평균적분흡광도(A)치:CA3구위38.87±1.24,치상회위42.35±1.40,태선섬유Timm염색과립적평균적분A치:1.38 ±0.10,여대조조비교,차이유통계학의의(P＜0.05).결론 해마구역GFAP표체화태상섬유출아증생재상후조기전간발작대서명현증가,여전간발작민감성밀절상관.
Objective To make model of early seizures after fluid percussion injury in brain of rats,to observe the expression of hippocampal glial fibrillary acidic protein (GFAP) and hippocampal mossy fibers,and to explore the early post-traumatic mechanism of epilepsy seizures.Methods Adult rats were divided into different groups.Experimental group was divided into three different sub-groups and fluid percussion brain injury model was made.GFAP in hippocampal area was detected on the 7th day after injury.Timm staining was carried out and observed under a microscope.Results Early seizures appeared in severe shock group.Average integral optical density of GFAP in the hippocampal region was 38.87 ± 1.24,and average integral optical density of Timm's staining granules was 1.38 ±0.10.As compared with control group,the difference was statistically significant (P ＜ 0.05).Conclusion The GFAP expression in the hippocampal region and mossy fiber spouting hyperplasia is significantly increased in rats with early seizures after injury,and closely related to the sensitivity of epileptic seizure.