中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
9期
1936-1938
,共3页
胰岛素样生长因子-1%骨折愈合%骨痂%骨质疏松症
胰島素樣生長因子-1%骨摺愈閤%骨痂%骨質疏鬆癥
이도소양생장인자-1%골절유합%골가%골질소송증
Insulin-like growth factor-1%Fracture healing%Callus%Osteoporosis
目的 观察胰岛素样生长因子-1(IGF-1)在家兔骨折愈合过程中的作用.方法 选择60只成年雌性新西兰白兔,按照随机数字分组法将其平均分为6组,每组10只,即正常对照组(N)、骨质疏松症对照组(0)以及4个实验组(A、B、C、D),除正常对照组外,其余5组家兔均摘除双侧卵巢后饲养12周,制备骨质疏松模型.将全部家兔制作左胫骨骨折模型,且于术后第1天开始,连续2d于骨折处按照0.2ml/100 g的注射剂量注射不同的注射液.N组与0组家兔注射三蒸水；A 、B、C、D4组注射IGF-1(注射剂量分别为l、10、100、1000 g/kg).于术后2、3、4、5周从每组抽取样本进行动态X线检查,左胫骨骨折处3点应力实验以及组织学观察.结果 (1)去卵巢手术前骨密度为(0.31 ±0.02) g/cm2,去卵巢术后骨密度为(0.27 ±0.01) g/cm2,两组差异有统计学意义(P＜0.05)；(2)影像学检查结果:术后2周,C、D两组均出现微量骨痂；术后3周,A、B、C、D各组均出现骨痂,且C、D两组骨折线变模糊；术后4周,C、D两组出现大量骨痂,且局部有增粗；术后5周,C、D两组骨折处出现大量的骨痂,骨密度明显增高,增粗区域明显增大,A、B两组骨折线均变模糊；(3)生物力学结果:术后5周,A、B、C、D组分别为(35.02 ±4.44)、(40.15 ±5.09)、(51.11 ±9.00)、(61.29±.9.18)N,实验组生物力学强度依次增大；(4)组织学结果:骨折后一定时间之内,各组的软骨细胞数目逐渐增多,且D组软骨细胞数目增长速度最快.结论 IGF-1体内局部注射能够明显促进骨质疏松骨折的愈合,且骨折愈合速度的快慢与IGF-1的注射剂量大小有关.
目的 觀察胰島素樣生長因子-1(IGF-1)在傢兔骨摺愈閤過程中的作用.方法 選擇60隻成年雌性新西蘭白兔,按照隨機數字分組法將其平均分為6組,每組10隻,即正常對照組(N)、骨質疏鬆癥對照組(0)以及4箇實驗組(A、B、C、D),除正常對照組外,其餘5組傢兔均摘除雙側卵巢後飼養12週,製備骨質疏鬆模型.將全部傢兔製作左脛骨骨摺模型,且于術後第1天開始,連續2d于骨摺處按照0.2ml/100 g的註射劑量註射不同的註射液.N組與0組傢兔註射三蒸水；A 、B、C、D4組註射IGF-1(註射劑量分彆為l、10、100、1000 g/kg).于術後2、3、4、5週從每組抽取樣本進行動態X線檢查,左脛骨骨摺處3點應力實驗以及組織學觀察.結果 (1)去卵巢手術前骨密度為(0.31 ±0.02) g/cm2,去卵巢術後骨密度為(0.27 ±0.01) g/cm2,兩組差異有統計學意義(P＜0.05)；(2)影像學檢查結果:術後2週,C、D兩組均齣現微量骨痂；術後3週,A、B、C、D各組均齣現骨痂,且C、D兩組骨摺線變模糊；術後4週,C、D兩組齣現大量骨痂,且跼部有增粗；術後5週,C、D兩組骨摺處齣現大量的骨痂,骨密度明顯增高,增粗區域明顯增大,A、B兩組骨摺線均變模糊；(3)生物力學結果:術後5週,A、B、C、D組分彆為(35.02 ±4.44)、(40.15 ±5.09)、(51.11 ±9.00)、(61.29±.9.18)N,實驗組生物力學彊度依次增大；(4)組織學結果:骨摺後一定時間之內,各組的軟骨細胞數目逐漸增多,且D組軟骨細胞數目增長速度最快.結論 IGF-1體內跼部註射能夠明顯促進骨質疏鬆骨摺的愈閤,且骨摺愈閤速度的快慢與IGF-1的註射劑量大小有關.
목적 관찰이도소양생장인자-1(IGF-1)재가토골절유합과정중적작용.방법 선택60지성년자성신서란백토,안조수궤수자분조법장기평균분위6조,매조10지,즉정상대조조(N)、골질소송증대조조(0)이급4개실험조(A、B、C、D),제정상대조조외,기여5조가토균적제쌍측란소후사양12주,제비골질소송모형.장전부가토제작좌경골골절모형,차우술후제1천개시,련속2d우골절처안조0.2ml/100 g적주사제량주사불동적주사액.N조여0조가토주사삼증수；A 、B、C、D4조주사IGF-1(주사제량분별위l、10、100、1000 g/kg).우술후2、3、4、5주종매조추취양본진행동태X선검사,좌경골골절처3점응력실험이급조직학관찰.결과 (1)거란소수술전골밀도위(0.31 ±0.02) g/cm2,거란소술후골밀도위(0.27 ±0.01) g/cm2,량조차이유통계학의의(P＜0.05)；(2)영상학검사결과:술후2주,C、D량조균출현미량골가；술후3주,A、B、C、D각조균출현골가,차C、D량조골절선변모호；술후4주,C、D량조출현대량골가,차국부유증조；술후5주,C、D량조골절처출현대량적골가,골밀도명현증고,증조구역명현증대,A、B량조골절선균변모호；(3)생물역학결과:술후5주,A、B、C、D조분별위(35.02 ±4.44)、(40.15 ±5.09)、(51.11 ±9.00)、(61.29±.9.18)N,실험조생물역학강도의차증대；(4)조직학결과:골절후일정시간지내,각조적연골세포수목축점증다,차D조연골세포수목증장속도최쾌.결론 IGF-1체내국부주사능구명현촉진골질소송골절적유합,차골절유합속도적쾌만여IGF-1적주사제량대소유관.
Objective To investigate the roles of insulin-like growth factor-1 (IGF-1) in rabbits fracture healing.Methods Sixty adult female New Zealand white rabbits were randomly divided into six groups according to the random number method (n =10 each):normal control group (N),osteoporosis control group (O),and four experiment groups (A,B,C,D).Except for the normal control group,the rats in the rest five groups were fed for 12 weeks after removal of both ovaries,resulting in osteoporosis model.The left tibia fracture model was made on all rabbits,and from the first day after surgery,0.2 ml/100 g dose were injected at the fracture in continuous 2 d.Group N,O rabbits injected with triple-distilled water; A,B,C,D four groups injected IGF-1 (injection doses were 1,10,100 and 1000 ng).In the postoperative 2,3,4 and 5 weeks samples from each group to do dynamic X ray examination,at three o'clock left tibial stress fracture experiments and histological observation.Results (1) BMD of ovariectomized before surgery (0.31 ± 0.02) g/cm2,bone mineral density after ovariectomy was (0.27 4 0.01) g/em2,the difference was statistically significant (P ＜ 0.05) ; (2) imaging findings:After 2 weeks,C,D groups were callus appears trace ; postoperative 3 weeks,A,B,C,D each group of bone callus,and C,D two fracture lines become blurred;postoperative 4 weeks,C,D groups a large number of callus and locally thickened;postoperative 5 weeks,C,D two massive fracture callus,bone density increased significantly,thickening region increased significantly,A,B two fracture lines are blurred ; (3) Biomechanical Results:N group,0 groups,A,B,C,D group were (40.14 ±4.15) N,(33.45±3.89) N,(35.02±4.44) N,(40.15 ±5.09) N,(51.11 ± 9.00) N,(61.29 ± 9.18) N,can be seen,increases in the order biomechanics.Conclusion IGF-1 in vivo injection can significantly promote the healing of osteoporotic fracture and fracture healing and speed of injection of IGF-1 there is a certain relationship between dose size.
