CAJ | 학술논문

目的探讨胃癌与肿瘤坏死因子相关凋亡诱导配体(TRAIL)基因多态性及单倍型的关系.方法收集321例胃癌患者及816例健康对照者,采用直接测序法检测TRAIL 3’非编码区(G1525A/C1595T)基因多态性,并行单倍型分析.结果与正常对照组比较,胃癌组中TRAILG1525A位点突变等位基因(A)和基因型(GA+AA)明显降低[30.37％(195/642)比53.92％(880/1632)；46.42％(149/321)比77.70％(634/816),P＜0.01]；且TRAIL C1595T位点突变等位基因(T)和基因型(CT+TT)亦显著降低[31.15％ (200/642)比55.39％(904/1632)；44.24％(142/321)比78.80％(643/816),P＜0.01].单倍型分析发现TRAIL(G1525A/C1595T)2个位点之间完全连锁,胃癌患者组中GT单倍型频率显著高于对照组(9.98％比0.21％,P＜0.01),而AT单倍型频率则明显低于对照组(42.45％比58.23％,P＜0.01).进一步采用非条件Logistic回归分析发现TRAIL C1595T基因突变与胃癌患者的病理分期相关,Ⅲ期+Ⅳ期胃癌患者中突变等位基因(T)和基因型(CT +TF)均显著高于Ⅰ期+Ⅱ期胃癌患者[36.59％ (150/410)比21.55％ (50/232);51.22％(105/205)比31.90％ (37/116)；P ＜0.01].结论 TRAIL (G1525A/C1595T)基因多态性及单倍型与胃癌相关.
목적 탐토위암여종류배사인자상관조망유도배체(TRAIL)기인다태성급단배형적관계.방법 수집321례위암환자급816례건강대조자,채용직접측서법검측TRAIL 3’비편마구(G1525A/C1595T)기인다태성,병행단배형분석.결과 여정상대조조비교,위암조중TRAILG1525A위점돌변등위기인(A)화기인형(GA+AA)명현강저[30.37％(195/642)비53.92％(880/1632)；46.42％(149/321)비77.70％(634/816),P＜0.01]；차TRAIL C1595T위점돌변등위기인(T)화기인형(CT+TT)역현저강저[31.15％ (200/642)비55.39％(904/1632)；44.24％(142/321)비78.80％(643/816),P＜0.01].단배형분석발현TRAIL(G1525A/C1595T)2개위점지간완전련쇄,위암환자조중GT단배형빈솔현저고우대조조(9.98％비0.21％,P＜0.01),이AT단배형빈솔칙명현저우대조조(42.45％비58.23％,P＜0.01).진일보채용비조건Logistic회귀분석발현TRAIL C1595T기인돌변여위암환자적병리분기상관,Ⅲ기+Ⅳ기위암환자중돌변등위기인(T)화기인형(CT +TF)균현저고우Ⅰ기+Ⅱ기위암환자[36.59％ (150/410)비21.55％ (50/232);51.22％(105/205)비31.90％ (37/116)；P ＜0.01].결론 TRAIL (G1525A/C1595T)기인다태성급단배형여위암상관.
Objective To investigate the associations of genetic polymorphisms and haplotypes of tumor necrosis factor-related apoptosis inducing ligand (TRAIL) with gastric cancer.Methods A total of 321 patients with gastric cancer and 816 healthy controls were recruited in this study.The genetic polymorphisms of TRAIL (G1525A/C1595T) genes were detected by direct sequencing.A haplotype analysis was also performed on all study subjects.Results Frequencies of variant allele (A) and genotype (GA + AA)in TRAIL G1525A were significantly lower in patients with gastric cancer than those in controls [30.37％ (195/642) vs.53.92％ (880/1632) ; 46.42％ (149/321) vs.77.70％ (634/816),both P ＜ 0.01].The same results were obtained from variant allele (T) and genotype (CT + TT) in TRAIL C1595T [31.15％ (200/642) vs.55.39％ (904/1632) ; 44.24％ (142/321) vs.78.80％ (643/816),both P ＜0.01].The haplotype analysis showed that TRAIL G1525A and C1595T were in complete linkage disequilibrium.The GT haplotype in patients with gastric cancer was significantly higher than that in controls (9.98％ vs.0.21％,P ＜0.01).However,the haplotype AT was significantly lower in patients with gastric cancer than in controls (42.45％ vs.58.23％,P ＜0.01).Unconditional Logistic regression analysis revealed that the mutation of TRAIL C1595T was significantly related to the clinicopathological grading of patients with gastric cancer.Frequencies of variant allele (T) and genotype (CT + TT) in TRAIL C1595T were significantly higher in patients with stage (Ⅲ + Ⅳ) gastric cancer than in those with stage (Ⅰ + Ⅱ)[36.59％ (150/410) vs.21.55％ (50/232) ; 51.22％ (105/205) vs.31.90％ (37/116) ; both P ＜0.01].Conclusion Genetic polymorphisms and haplotypes of TRAIL (G1525A/C1595T) are significantly correlated with gastric cancer.