中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
4期
723-725
,共3页
李坤%翟水亭%周瑜%张志东%史帅涛%刘长建%梁凯
李坤%翟水亭%週瑜%張誌東%史帥濤%劉長建%樑凱
리곤%적수정%주유%장지동%사수도%류장건%량개
聚四氟乙烯%人工血管%介孔%肝素%生物相容性
聚四氟乙烯%人工血管%介孔%肝素%生物相容性
취사불을희%인공혈관%개공%간소%생물상용성
Expanded polytetrafluoroethylene%Artificial vascular prosthesis%Mesoporous%Heparin%Biocompatibility
目的 制备负载肝素的介孔膨体聚四氟乙烯(ePTFE)人工血管并观察其生物相容性.方法 实验分为介孔人工血管组和普通人工血管组.制备不同性能的固载肝素的介孔人工血管,标记为HMVP-n(n=1,2,3).利用凝血酶失活实验、加压灌注实验、蛋白吸附实验和乳酸脱氢酶(LDH)细胞毒性实验进行检测.结果 成功制备出固载肝素的介孔人工血管.凝血酶失活实验显示:HMBVP-n在分别释放5d和20d后仍能灭活大部分凝血酶,HMBVP-1能保持更好的肝素释放性能(P＜0.05).加压灌注实验显示合成血管无明显直径和长度的变化(P＞0.05),HMVP-n纤维蛋白的吸附值(OD值)分别为0.29 ±0.12、0.31±0.06、0.30±0.07,较对照组(0.56±0.23)明显地减少了吸附量(P＜0.05),而白蛋白在HMVP-n人工血管吸附的OD值分别为0.390±0.150、0.327±0.210、0.332±0.240,较对照组(0.291 ±0.220)更易于白蛋白的吸附(P＜0.05),其中HMVP-1最为明显,差异有统计学意义(P＜0.05).细胞毒性实验显示合成血管LDH的吸光度值分别为0.541 ±0.008、0.583±0.012、0.587 ±0.009,与对照组(0.624±0.011)比较差异无统计学意义(P＞0.05).结论 肝素固载的含介孔人工血管具有良好的长期抗凝性和生物相容性.
目的 製備負載肝素的介孔膨體聚四氟乙烯(ePTFE)人工血管併觀察其生物相容性.方法 實驗分為介孔人工血管組和普通人工血管組.製備不同性能的固載肝素的介孔人工血管,標記為HMVP-n(n=1,2,3).利用凝血酶失活實驗、加壓灌註實驗、蛋白吸附實驗和乳痠脫氫酶(LDH)細胞毒性實驗進行檢測.結果 成功製備齣固載肝素的介孔人工血管.凝血酶失活實驗顯示:HMBVP-n在分彆釋放5d和20d後仍能滅活大部分凝血酶,HMBVP-1能保持更好的肝素釋放性能(P＜0.05).加壓灌註實驗顯示閤成血管無明顯直徑和長度的變化(P＞0.05),HMVP-n纖維蛋白的吸附值(OD值)分彆為0.29 ±0.12、0.31±0.06、0.30±0.07,較對照組(0.56±0.23)明顯地減少瞭吸附量(P＜0.05),而白蛋白在HMVP-n人工血管吸附的OD值分彆為0.390±0.150、0.327±0.210、0.332±0.240,較對照組(0.291 ±0.220)更易于白蛋白的吸附(P＜0.05),其中HMVP-1最為明顯,差異有統計學意義(P＜0.05).細胞毒性實驗顯示閤成血管LDH的吸光度值分彆為0.541 ±0.008、0.583±0.012、0.587 ±0.009,與對照組(0.624±0.011)比較差異無統計學意義(P＞0.05).結論 肝素固載的含介孔人工血管具有良好的長期抗凝性和生物相容性.
목적 제비부재간소적개공팽체취사불을희(ePTFE)인공혈관병관찰기생물상용성.방법 실험분위개공인공혈관조화보통인공혈관조.제비불동성능적고재간소적개공인공혈관,표기위HMVP-n(n=1,2,3).이용응혈매실활실험、가압관주실험、단백흡부실험화유산탈경매(LDH)세포독성실험진행검측.결과 성공제비출고재간소적개공인공혈관.응혈매실활실험현시:HMBVP-n재분별석방5d화20d후잉능멸활대부분응혈매,HMBVP-1능보지경호적간소석방성능(P＜0.05).가압관주실험현시합성혈관무명현직경화장도적변화(P＞0.05),HMVP-n섬유단백적흡부치(OD치)분별위0.29 ±0.12、0.31±0.06、0.30±0.07,교대조조(0.56±0.23)명현지감소료흡부량(P＜0.05),이백단백재HMVP-n인공혈관흡부적OD치분별위0.390±0.150、0.327±0.210、0.332±0.240,교대조조(0.291 ±0.220)경역우백단백적흡부(P＜0.05),기중HMVP-1최위명현,차이유통계학의의(P＜0.05).세포독성실험현시합성혈관LDH적흡광도치분별위0.541 ±0.008、0.583±0.012、0.587 ±0.009,여대조조(0.624±0.011)비교차이무통계학의의(P＞0.05).결론 간소고재적함개공인공혈관구유량호적장기항응성화생물상용성.
Objective To fabricate the mesoporous-based heparin-loaded expanded polytetrafluoroethylene (ePTFE) vascular prosthesis and evaluate their biocompatibility.Methods The study included the mesoporous-based vascular prosthesis group and the control group.The chromogenic assay.Pressing-perfusion test,protein adsorption assay and lactate dehydrogenase (LDH) cytotoxicity assay were determined to evaluate hemocompatibility and compatibility of vascular prosthesis.Results The heparin-released mesoporous-based vascular prosthesis weas synthesized successfully.The chromogenic assay confirmed that heparin-loaded keep lasting activity than the control vascular prosthesis group (P ＜ 0.05).HMVP-n keep compliance of original vascular prosthesis (P ＞ 0.05).The adsorption of fibrinogen on the surface of HMVP-n membranes is effectively reduced (OD values:0.29 ±0.12,0.31 ±0.06 and 0.30 ± 0.07,respectively),but HMVP-n grafts attracted higher amount of albumin (OD values:0.390 ± 0.150,0.327 ± 0.210 and 0.332 ± 0.240,respectively) compared than unmodified ePTFE graft (P ＜ 0.05),HMVP-1 especially.there was not significant difference of A values between HMVP-n and unmodified material (P ＞0.05) by LDH Cytotoxicity Assay.Conclusion The heparin-released mesoporous-based vascular prosthesis keep lasting antithrombotic activity and has good biocompatibility.
