中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
4期
768-770
,共3页
过氧化损伤%丹酚酸A%肝脏疾病
過氧化損傷%丹酚痠A%肝髒疾病
과양화손상%단분산A%간장질병
Peroxidation injury%Salvianolic acid A%Liver disease
目的 筛选建立正常人肝细胞(L02细胞)过氧化损伤模型适宜的过氧化氢(H2O2)浓度及丹酚酸A的体外适宜肝细胞保护作用浓度及时间.方法 将常规培养的L02细胞分为3部分:(1)以不同浓度的H2O2处理L02细胞;(2)以不同浓度的丹酚酸A处理L02细胞;(3)以不同浓度丹酚酸A预处理L02细胞24 h后加入H2O2.噻唑蓝(MTT)法检测并在光镜下观察各组细胞生长状态.结果 当H2O2浓度为160 μmol/L时,细胞生长抑制率约为35％,部分细胞漂浮并出现少量细胞碎片;20 nmol/L丹酚酸A直接作用L02细胞在24 h平均吸光度值为(1.7043 ±0.008 1),明显高于对照组(1.5443±0.008 3,P＜0.01);20 nmol/L丹酚酸A预处理L02细胞后加入H2O24 h平均吸光度值为(1.565 0 ±0.041 6),在各组中最高,与对照组(1.5700±0.021 2)比较差异无统计学意义(P＞0.05).结论 160μmol/L是H2O2建立过氧化损伤细胞模型的较适宜浓度;20 nmol/L丹酚酸A预处理L02细胞24 h可促进细胞增殖,改善H2O2造成的L02细胞过氧化损伤.
目的 篩選建立正常人肝細胞(L02細胞)過氧化損傷模型適宜的過氧化氫(H2O2)濃度及丹酚痠A的體外適宜肝細胞保護作用濃度及時間.方法 將常規培養的L02細胞分為3部分:(1)以不同濃度的H2O2處理L02細胞;(2)以不同濃度的丹酚痠A處理L02細胞;(3)以不同濃度丹酚痠A預處理L02細胞24 h後加入H2O2.噻唑藍(MTT)法檢測併在光鏡下觀察各組細胞生長狀態.結果 噹H2O2濃度為160 μmol/L時,細胞生長抑製率約為35％,部分細胞漂浮併齣現少量細胞碎片;20 nmol/L丹酚痠A直接作用L02細胞在24 h平均吸光度值為(1.7043 ±0.008 1),明顯高于對照組(1.5443±0.008 3,P＜0.01);20 nmol/L丹酚痠A預處理L02細胞後加入H2O24 h平均吸光度值為(1.565 0 ±0.041 6),在各組中最高,與對照組(1.5700±0.021 2)比較差異無統計學意義(P＞0.05).結論 160μmol/L是H2O2建立過氧化損傷細胞模型的較適宜濃度;20 nmol/L丹酚痠A預處理L02細胞24 h可促進細胞增殖,改善H2O2造成的L02細胞過氧化損傷.
목적 사선건립정상인간세포(L02세포)과양화손상모형괄의적과양화경(H2O2)농도급단분산A적체외괄의간세포보호작용농도급시간.방법 장상규배양적L02세포분위3부분:(1)이불동농도적H2O2처리L02세포;(2)이불동농도적단분산A처리L02세포;(3)이불동농도단분산A예처리L02세포24 h후가입H2O2.새서람(MTT)법검측병재광경하관찰각조세포생장상태.결과 당H2O2농도위160 μmol/L시,세포생장억제솔약위35％,부분세포표부병출현소량세포쇄편;20 nmol/L단분산A직접작용L02세포재24 h평균흡광도치위(1.7043 ±0.008 1),명현고우대조조(1.5443±0.008 3,P＜0.01);20 nmol/L단분산A예처리L02세포후가입H2O24 h평균흡광도치위(1.565 0 ±0.041 6),재각조중최고,여대조조(1.5700±0.021 2)비교차이무통계학의의(P＞0.05).결론 160μmol/L시H2O2건립과양화손상세포모형적교괄의농도;20 nmol/L단분산A예처리L02세포24 h가촉진세포증식,개선H2O2조성적L02세포과양화손상.
Objective To select the appropriate concentration of hydrogen peroxide for the establishment of cell peroxidation damage model,and the applicable concentration and time of salvianolic acid A for protection.Methods The L02 ceils were divided into 3 groups.In group 1,L02 cells were subjected to hydrogen peroxide for 24 h with different concentrations.In group 2,L02 cells were treated with different concentrations of salvianolic acid A.In group 3,hydrogen peroxide was used to deal with L02 cells pretreated by salvianolic acid A with different concentrations for 24 h.Methyl thiazolyl tetrazolium (MTT) assay was used to measure the growth of L02 cells under a microscope.Results The inhibition ratio of growth of L02 cells treated with 160 μmol/L H2O2 was 35％,and part of the cells started to float and sporadic cell debris appeared.In group 2,the mean optical density was (1.704 3 ± 0.008 1) at 24 h in 20 nmol/L group,which was the highest,and significantly higher than in control group (1.544 3 ± 0.008 3,P ＜ 0.01).The mean optical density in group 3 was (1.565 0 ±0.041 6),which showed no significant difference from control groups (1.570 0-± 0.021 2,P ＞ 0.05).The mean optical density in the remaining groups at each time point was all below the control groups.Conclusion 160 μmol/L was the most suitable concentration for H2O2 to establish the cell peroxidation damage model.20 nmol/L salvianolic acid A could not only accelerate cell proliferation,but also alleviate the damage caused by hydrogen peroxide.
