中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
4期
771-773
,共3页
张燕%谭延伟%蔡建辉%胡玲玲
張燕%譚延偉%蔡建輝%鬍玲玲
장연%담연위%채건휘%호령령
树突状细胞%细胞毒性T细胞%黑色素瘤%抑瘤率
樹突狀細胞%細胞毒性T細胞%黑色素瘤%抑瘤率
수돌상세포%세포독성T세포%흑색소류%억류솔
Dendritic cell%Cytotoxic T lymphocyte%Melanoma%Inhibition rate of tumor
目的 探讨CD4+ CD25+ Treg细胞对树突状细胞(DC)诱导的细胞毒性T细胞(CTL)对B16黑色素瘤抑瘤作用的影响及其机制.方法 制备C57BL/6小鼠B16黑色素瘤动物模型,行全身放疗达非髓性淋巴细胞删除(NMLD)状态.随机将80只小鼠分为4组.A组:在DC与T细胞共同培养前应用免疫磁珠法(MACS)删除CD4+ CD25+ Treg细胞;B组:DC与T细胞共同培养后删除CD4+ CD25+ Treg细胞;C组:DC与T细胞共同培养时不删除CD4+ CD25+ Treg细胞.D组:对照组.体外应用DC诱导T细胞产生效应细胞CTL,将CTL经鼠尾静脉回输小鼠体内,观察对黑色素瘤的抑瘤效果.结果 A、B、C3组对B16黑色素瘤抑瘤率分别为90.89％、92.52％、60.02％,均明显高于对照组(P＜0.05).删除CD4+ CD25+ Treg细胞的A、B两组抑瘤率优于C组(P＜0.05).但A、B两组间抑瘤率差异无统计学意义(P＞0.05).结论 删除CD4+ CD25+ Treg细胞可明显提高CTL对B16黑色素瘤的抑瘤效果.
目的 探討CD4+ CD25+ Treg細胞對樹突狀細胞(DC)誘導的細胞毒性T細胞(CTL)對B16黑色素瘤抑瘤作用的影響及其機製.方法 製備C57BL/6小鼠B16黑色素瘤動物模型,行全身放療達非髓性淋巴細胞刪除(NMLD)狀態.隨機將80隻小鼠分為4組.A組:在DC與T細胞共同培養前應用免疫磁珠法(MACS)刪除CD4+ CD25+ Treg細胞;B組:DC與T細胞共同培養後刪除CD4+ CD25+ Treg細胞;C組:DC與T細胞共同培養時不刪除CD4+ CD25+ Treg細胞.D組:對照組.體外應用DC誘導T細胞產生效應細胞CTL,將CTL經鼠尾靜脈迴輸小鼠體內,觀察對黑色素瘤的抑瘤效果.結果 A、B、C3組對B16黑色素瘤抑瘤率分彆為90.89％、92.52％、60.02％,均明顯高于對照組(P＜0.05).刪除CD4+ CD25+ Treg細胞的A、B兩組抑瘤率優于C組(P＜0.05).但A、B兩組間抑瘤率差異無統計學意義(P＞0.05).結論 刪除CD4+ CD25+ Treg細胞可明顯提高CTL對B16黑色素瘤的抑瘤效果.
목적 탐토CD4+ CD25+ Treg세포대수돌상세포(DC)유도적세포독성T세포(CTL)대B16흑색소류억류작용적영향급기궤제.방법 제비C57BL/6소서B16흑색소류동물모형,행전신방료체비수성림파세포산제(NMLD)상태.수궤장80지소서분위4조.A조:재DC여T세포공동배양전응용면역자주법(MACS)산제CD4+ CD25+ Treg세포;B조:DC여T세포공동배양후산제CD4+ CD25+ Treg세포;C조:DC여T세포공동배양시불산제CD4+ CD25+ Treg세포.D조:대조조.체외응용DC유도T세포산생효응세포CTL,장CTL경서미정맥회수소서체내,관찰대흑색소류적억류효과.결과 A、B、C3조대B16흑색소류억류솔분별위90.89％、92.52％、60.02％,균명현고우대조조(P＜0.05).산제CD4+ CD25+ Treg세포적A、B량조억류솔우우C조(P＜0.05).단A、B량조간억류솔차이무통계학의의(P＞0.05).결론 산제CD4+ CD25+ Treg세포가명현제고CTL대B16흑색소류적억류효과.
Objective To investigate the role of CD4 + CD25 + regulatory T cell (Treg) in cytotoxic T lymphocyte (CTL) inhibiting B16 melanoma and the mechanism.Methods B16 melanoma cells were injected into subcutaneous tissue under the left shoulder of the C57BL/6 mouse to make the melanoma animal models.Then the animal models were irradiated by 60Co-ray on the total body in order to get a no-marrow lymphocyte deletion (NMLD) state.Eighty C57BL/6 mice were divided into 4 groups:3 experimental groups and a control group,with 20 mice each.In vitro T cells were induced into CTL with tumor specificity.CTL were injected back into the mouse body through the vein in the tail.The changes of tumor volume and tumor weight were observed.Results The tumor inhibition rate in the three experiment groups was 90.89％,92.52％ and 60.02％ respectively,which was significantly higher than that in the control group (P ＜ 0.05).A significant tumor inhibition was observed in groups A and B with a deletion of CD4 + CD25 + Tregs,in comparison with that in group C with no deletion of Tregs (P ＜ 0.05).But no significant difference was found between groups A and B with deletion of Tregs at different time points (P ＞ 0.05).Conclusion The deletion of CD4 + CD25 + Treg could significantly increase the tumor inhibition effect of antigen-specific CTL in melanoma.
