CAJ | 학술논문

目的探讨混合脐血间充质干细胞(UCB-MSCs)体外抑制C6胶质瘤细胞增殖机制.方法采用免疫组织化学检测C6细胞B细胞淋巴瘤/白血病-2(bcl-2)和半胱氨酰天冬氨酸特异性蛋白酶(Caspase)-3蛋白的表达,E/T(E:效应细胞即UCB-MSCs,T:靶细胞即C6细胞)=0∶1、10∶1、(5+5)∶1时,采用免疫组织化学检测C6细胞的bcl-2和Caspase-3蛋白的表达.结果混合UCB-MSCs对C6细胞在蛋白表达上有使凋亡蛋白Caspase-3表达增高的趋势,抑凋亡蛋白bcl-2表达降低的趋势;促凋亡蛋白表达量和凋亡细胞数随E/T的比例增高而增高,呈现比例依赖关系;当E/T比例相同时,混合组[E/T=(5+5)∶1,(56±5)％]UCB-MSCs促凋亡蛋白表达量比单份组[E/T=10∶1,(33±6)％]高;抑凋亡蛋白表达量混合组[E/T=(5+5)∶1,(27±3)％],单份组[E/T=10∶1,(46±7)％].结论与单份组比较,混合UCB-MSCs对体外C6细胞更具有抑制增殖作用.
목적 탐토혼합제혈간충질간세포(UCB-MSCs)체외억제C6효질류세포증식궤제.방법 채용면역조직화학검측C6세포B세포림파류/백혈병-2(bcl-2)화반광안선천동안산특이성단백매(Caspase)-3단백적표체,E/T(E:효응세포즉UCB-MSCs,T:파세포즉C6세포)=0∶1、10∶1、(5+5)∶1시,채용면역조직화학검측C6세포적bcl-2화Caspase-3단백적표체.결과 혼합UCB-MSCs대C6세포재단백표체상유사조망단백Caspase-3표체증고적추세,억조망단백bcl-2표체강저적추세;촉조망단백표체량화조망세포수수E/T적비례증고이증고,정현비례의뢰관계;당E/T비례상동시,혼합조[E/T=(5+5)∶1,(56±5)％]UCB-MSCs촉조망단백표체량비단빈조[E/T=10∶1,(33±6)％]고;억조망단백표체량혼합조[E/T=(5+5)∶1,(27±3)％],단빈조[E/T=10∶1,(46±7)％].결론 여단빈조비교,혼합UCB-MSCs대체외C6세포경구유억제증식작용.
Objective Study on the mechanisms of the mixed umbilical cord blood-derived mesenchymal stem cells in inhibiting proliferation of C6 glioma cells.It provided experimental and theoretical basis for the mixed umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) treatment of C6 glioma in vivo.Methods B cell lymphoma/leukemia-2 (bcl-2) and Cysteinyl aspartate-specific protease-3 (Caspase-3) protein expression of C6 cells were analyzed by immunohistochemistry.Results The mixed UCB-MSCs inhibited the proliferation of enhanced green fluorescent protein (EGFP)-C6 glioma cells in vitro.When the E/T ratio is same,Expression of Caspase-3 in the mixed group [E/T =(5 + 5)∶1,(56 ± 5)％],but the single group is [E/T =10∶ 1,(33 ± 6) ％] ; Expression of bcl-2 in mixed group is [E/T =(5 + 5) ∶ 1,(27 ± 3) ％],but a single group is [E/T =10∶ 1,(46 ± 7) ％].Caspase-3 protein expression tended to increase,however,bcl-2 protein expression decreased.Conclusion Compared with the single group,the mixed UCB-MSCs could inhibit proliferation of C6 cells in vitro.