CAJ | 학술논문

目的观察同种异体肋软骨细胞复合透明质酸钠对兔膝关节全层软骨缺损的修复作用.方法分离、培养肋软骨细胞,鉴定生物学活性.将36只新西兰大白兔制备膝关节全层软骨缺损模型并随机分为3组(n=12).空白组不进行特殊处理;对照组注入透明质酸钠;实验组注入肋软骨细胞/透明质酸钠复合物.术后第1、2、3个月各组分别随机处死4只兔并获取股骨远端标本,行肉眼大体观察、病理组织学检测,按O' Driseoll,keeley and salter法行组织学评分.结果第2代肋软骨细胞阿尔辛蓝、番红O、Ⅱ型胶原染色均呈强阳性.实验组术后第1、2、3个月组织学评分(8.75000±0.50000、14.000 00±0.816 50、19.00000±0.81650)明显高于对照组(0.75000±0.50000、2.00000±0.81650、5.00000±0.816 50)和空白组(0.50000±0.577 35、0、0),同一时间点实验组与对照组和空白组组织学评分比较差异均有统计学意义(P＜0.05).结论第2代肋软骨细胞适合作为软骨组织工程的种子细胞;肋软骨细胞复合透明质酸钠修复兔膝关节全层软骨缺损近期效果满意.
목적 관찰동충이체륵연골세포복합투명질산납대토슬관절전층연골결손적수복작용.방법 분리、배양륵연골세포,감정생물학활성.장36지신서란대백토제비슬관절전층연골결손모형병수궤분위3조(n=12).공백조불진행특수처리;대조조주입투명질산납;실험조주입륵연골세포/투명질산납복합물.술후제1、2、3개월각조분별수궤처사4지토병획취고골원단표본,행육안대체관찰、병리조직학검측,안O' Driseoll,keeley and salter법행조직학평분.결과 제2대륵연골세포아이신람、번홍O、Ⅱ형효원염색균정강양성.실험조술후제1、2、3개월조직학평분(8.75000±0.50000、14.000 00±0.816 50、19.00000±0.81650)명현고우대조조(0.75000±0.50000、2.00000±0.81650、5.00000±0.816 50)화공백조(0.50000±0.577 35、0、0),동일시간점실험조여대조조화공백조조직학평분비교차이균유통계학의의(P＜0.05).결론 제2대륵연골세포괄합작위연골조직공정적충자세포;륵연골세포복합투명질산납수복토슬관절전층연골결손근기효과만의.
Objective To explore the effects of allogeneic composites of costal chondrocyte/Sodium hyaluronate in repairing the full-thickness cartilage defects at knee joints of rabbit models.Methods Condrocytes were isolated and cultivated for evalutating biological activities.Full-thickness cartilage defect models from knee joints of 36 Newzealand rabbits were randomly and evenly allocated into blank,control and experimental groups.The blank group was subjected to no disposal.For control group,sodium hyaluronate was injected into the defect region,and costal chondrocytes/sodium hyaluronate composites were injected into the defect region in experimental group.Every 4 rabbits were executed randomly by the end of the first,second and third month respectively for specimens sampled from distal femur and the specimens were obsevered under direct vision,examined by histological staining and scored according to O' Driseoll,keeley and salter scales respectively.Results The 2nd generation costal chondrocytes were remarkably stained by alcian blue,sarranine O and collagen (type Ⅱ) respectively.The histological scores of experimental group (8.750 00 ± 0.500 00,14.000 00 ± 0.816 50,19.000 00 ± 0.816 50) were significantly higher than those of blank group (0.750 00 ±0.500 00,2.000 00 ±0.816 50,5.000 00 ±0.816 50) and blank group (0.500 00 ± 0.577 35,0,0) based upon statistical differences (P ＜ 0.05).Conclusion Costal chondrocytes of the 2nd genretation is qualified as seed cells for cartilage tissue engineering research.Allogeneic costal chondrocyte/sodium hyaluronate composites contribute to restore full-thickness cartilage defect at knee joints of the rabbit models satisfactorily.