CAJ | 학술논문

目的探讨积雪草酸对大鼠急性脊髓损伤的保护作用及其机制.方法将45只SD大鼠随机分为假手术组、模型组及积雪草酸治疗组,在损伤后24、48、72 h先行后肢运动功能BBB评分,随后处死取材.通过苏木素-伊红(HE)染色观察脊髓组织病理变化,检测脊髓匀浆超氧化物歧化酶(SOD)、髓过氧化物酶(MPO)活性、丙二醛(MDA)、白细胞介素(IL)-6水平.结果术后积雪草组大鼠后肢运动功能较模型组均有所改善,48 h和72h时差异有统计学意义(P＜0.05).术后48 h模型组SOD活性[(175.32 ±3.45) U/mg]较假手术组[(253.46±5.69) U/mg]明显降低(P＜0.05),而MDA含量[(7.61 ±0.24) nmol/mg]较假手术组[(2.69 ±0.29) nmoL/mg]明显增加(P＜0.05);积雪草治疗组术后48 h SOD活性[(231.44 ±4.88) U/mg]较模型组[(175.32 ±3.45) U/mg]升高(P＜0.05),MDA则呈现相反的趋势(P＜0.05).造模后脊髓组织中IL-6含量、MPO活性均随时间推移逐渐增高,而积雪草酸组在各时间点均较模型组有所降低,且差异有统计学意义(P＜0.05).积雪草酸治疗组组织学表现优于模型组.结论积雪草酸通过抑制氧化应激和炎性反应,而发挥对大鼠急性脊髓损伤的保护作用.
목적 탐토적설초산대대서급성척수손상적보호작용급기궤제.방법 장45지SD대서수궤분위가수술조、모형조급적설초산치료조,재손상후24、48、72 h선행후지운동공능BBB평분,수후처사취재.통과소목소-이홍(HE)염색관찰척수조직병리변화,검측척수균장초양화물기화매(SOD)、수과양화물매(MPO)활성、병이철(MDA)、백세포개소(IL)-6수평.결과 술후적설초조대서후지운동공능교모형조균유소개선,48 h화72h시차이유통계학의의(P＜0.05).술후48 h모형조SOD활성[(175.32 ±3.45) U/mg]교가수술조[(253.46±5.69) U/mg]명현강저(P＜0.05),이MDA함량[(7.61 ±0.24) nmol/mg]교가수술조[(2.69 ±0.29) nmoL/mg]명현증가(P＜0.05);적설초치료조술후48 h SOD활성[(231.44 ±4.88) U/mg]교모형조[(175.32 ±3.45) U/mg]승고(P＜0.05),MDA칙정현상반적추세(P＜0.05).조모후척수조직중IL-6함량、MPO활성균수시간추이축점증고,이적설초산조재각시간점균교모형조유소강저,차차이유통계학의의(P＜0.05).적설초산치료조조직학표현우우모형조.결론 적설초산통과억제양화응격화염성반응,이발휘대대서급성척수손상적보호작용.
Objective To observe the effect of asiatic aicd treatment on acute spinal cord injury in rats.Methods Forty-five SD rats were randomly divided into a sham-operation group,model control group and asiatic acid treatment group.Functional outcome measurements were evaluated by the Basso,Beattie and Bresnahan (BBB) score at 24,48 and 72 h.The sections were stained with hematoxylin and eosin for morphological and histopathological analyses.The reactive oxidative species and inflammatory factor were detected.Results The BBB score was significantly higher in the asiatic acid treatment group (6.70 ± 1.09 and 9.58 ±2.81) than in the model control group (3.05 ±0.98 and 4.55 ±0.65) at 48 h and 72 h (P ＜ 0.05).Compared to the model control,HE staining showed the histopathological performance was improved greatly in rat spinal cord in asiatic acid treatment group.At the three time points,tissue malondialdehyde (MDA) content in asiatic acid treatment group [(6.42 ± 0.15),(6.17 ± 0.25) and (5.48 ± 0.33) nmol/ml] was statistically significantly lower than in the model group [(7.55 ±0.37),(7.61 ± 0.24) and (6.59 ±0.22) nmol/ml].The superoxide dismutase (SOD) activity in the asiatic acid treatment group [(220.55 ±3.76),(231.44 ±4.88) and (235.98 ±5.26) U/mg] was higher than in the model group [(180.31 ±4.68,175.32 ±3.45 and 170.51 ±3.44) U/mg] at the three time points.After injury,tissue myeloperoxidase (MPO) and IL contents were increased,and showed a significant difference between the model control group and asiatic acid treatment group at the three time points.Conclusion The present study suggests that asiatic acid exerts neuroprotection by reducing oxidative stress.