中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
4期
832-834
,共3页
陈虎%郭卫春%戢鹏%余铃
陳虎%郭衛春%戢鵬%餘鈴
진호%곽위춘%집붕%여령
U2OS细胞株%γ-分泌酶抑制剂%骨肉瘤
U2OS細胞株%γ-分泌酶抑製劑%骨肉瘤
U2OS세포주%γ-분비매억제제%골육류
U2OS cells%γ-secretase inhibitors%Osteosarcoma
目的 观察Notch通路抑制剂对骨肉瘤细胞U2OS的作用.方法 常规培养U2OS细胞,应用RO4929097处理U2OS细胞,通过细胞计数试剂盒(CCK-8)法观察RO4929097对U2OS细胞的毒性作用,通过流式细胞仪检测RO4929097对U2OS细胞周期的影响及诱导凋亡的作用.结果 CCK-8检测结果显示不同浓度RO4929097对U2OS细胞有明显的抑制作用,与对照组比较差异有统计学意义(P＜0.05),流式细胞检测结果显示RO4929097能促进U2OS细胞凋亡而不影响细胞周期.结论 Notch通路抑制剂可诱导U2OS凋亡.
目的 觀察Notch通路抑製劑對骨肉瘤細胞U2OS的作用.方法 常規培養U2OS細胞,應用RO4929097處理U2OS細胞,通過細胞計數試劑盒(CCK-8)法觀察RO4929097對U2OS細胞的毒性作用,通過流式細胞儀檢測RO4929097對U2OS細胞週期的影響及誘導凋亡的作用.結果 CCK-8檢測結果顯示不同濃度RO4929097對U2OS細胞有明顯的抑製作用,與對照組比較差異有統計學意義(P＜0.05),流式細胞檢測結果顯示RO4929097能促進U2OS細胞凋亡而不影響細胞週期.結論 Notch通路抑製劑可誘導U2OS凋亡.
목적 관찰Notch통로억제제대골육류세포U2OS적작용.방법 상규배양U2OS세포,응용RO4929097처리U2OS세포,통과세포계수시제합(CCK-8)법관찰RO4929097대U2OS세포적독성작용,통과류식세포의검측RO4929097대U2OS세포주기적영향급유도조망적작용.결과 CCK-8검측결과현시불동농도RO4929097대U2OS세포유명현적억제작용,여대조조비교차이유통계학의의(P＜0.05),류식세포검측결과현시RO4929097능촉진U2OS세포조망이불영향세포주기.결론 Notch통로억제제가유도U2OS조망.
Objective To explore the effects of γ-secretase inhibitors on U2OS cells.Methods U2OS cells cultured under normoxic conditions were treated with RO4929097.The cytotoxicity was detected by cell counting Kit-8 (CCK-8) kit.The change of cell cycle and apoptosis were assayed by flow cytometry (FCM).Results The present study showed that RO4929097 has an obvious inhibition effect on U2OS cells.It had also been found that the apoptosis was aggravated while the cell cycle wasn' t disturbed by RO4929097.Conclusion The apoptosis of U2OS cells could be aggravated by γ-secretase inhibitors.
