CAJ | 학술논문

目的探讨长链非编码RNA (LncRNA)在肝细胞肝癌(以下简称肝癌)组织中的表达及其临床意义.方法采用实时定量聚合酶链反应(Real-time PCR)法检测7种与肿瘤相关LncRNA[B细胞整合簇基因(BIC)、淋巴细胞白血病中删除基因1(DLEU1)、小核RNA看家基因5(SNHG5)、反义胰岛素样生长因子2(IGF2AS)、前列腺癌基因3(PCA3)、α-缺氧诱导因子(HIF-α)、前列腺癌基因表达标记1(PCGEM1)]在肝癌组织和癌旁组织中(n=40)的表达.结果 SNHG5、IGF2AS、PCA3、HIF-α在肝癌组织和癌旁组织中的表达差异有统计学意义(P＜0.01);其中IGF2AS为表达差异最显著的LncRNA[肝癌:10.910±1.775)比癌旁组织(13.517±3.077)],且在肝癌组织中的表达与肿瘤数目(P＜0.05)和微血管侵犯(P＜0.01)密切相关.Cox多因素分析显示,IGF2AS在肿瘤组织中的表达是肝癌患者无瘤生存的独立预后因素.结论新的LncRNA如IGF2AS在肝癌发生发展及预后中的重要作用值得进一步研究.
목적 탐토장련비편마RNA (LncRNA)재간세포간암(이하간칭간암)조직중적표체급기림상의의.방법 채용실시정량취합매련반응(Real-time PCR)법검측7충여종류상관LncRNA[B세포정합족기인(BIC)、림파세포백혈병중산제기인1(DLEU1)、소핵RNA간가기인5(SNHG5)、반의이도소양생장인자2(IGF2AS)、전렬선암기인3(PCA3)、α-결양유도인자(HIF-α)、전렬선암기인표체표기1(PCGEM1)]재간암조직화암방조직중(n=40)적표체.결과 SNHG5、IGF2AS、PCA3、HIF-α재간암조직화암방조직중적표체차이유통계학의의(P＜0.01);기중IGF2AS위표체차이최현저적LncRNA[간암:10.910±1.775)비암방조직(13.517±3.077)],차재간암조직중적표체여종류수목(P＜0.05)화미혈관침범(P＜0.01)밀절상관.Cox다인소분석현시,IGF2AS재종류조직중적표체시간암환자무류생존적독립예후인소.결론 신적LncRNA여IGF2AS재간암발생발전급예후중적중요작용치득진일보연구.
Objective To investigate the expression of long chain non-coding RNA (LncRNA) in hepatocellular carcinoma tissue and its clinical significance.Methods Real-time quantitative polymerase chain reaction (Real-time PCR) method was used to detect the expression of seven kinds of LncRNA associated with tumor [B cell integration cluster gene(BIC),deleted in lymphocytic leukemia 1 (DLEU1),small nucleolar RNA host genes 5 (SNHG5),insulin-like growth factor 2,antisense (IGF2AS),prostate cancer gene 3 (PCA3),Hypoxia inducible factor α (HIF-α) and Prostate cancer gene expression marker 1 (PCGEM1)] in liver cancer tissues and adjacent tissues (n =40).Results The expression of SNHG5,IGF2AS,PCA3,and HIF-α in liver cancer tissues and adjacent tissues was statistically significantly different (respectively P ＜ 0.01).IGF2AS was most significantly differentially expressed [HCC (10.910 ± 1.775) vs.paratumor (13.517 ± 3.077)],and its expression in liver cancer tissue was closely related to tumor number (P ＜ 0.05) and microvascular invasion (P ＜ 0.01).Cox multi-factor analysis showed that the expression of IGF2AS in tumor tissues was an independent prognostic factors of the liver disease-free survival.Conclusion The important role of IGF2AS played in HCC development and prognosis is worthy of further study.