CAJ | 학술논문

目的探讨CD4+T细胞三磷酸腺苷(ATP)值及T淋巴细胞亚群测定在肝移植术后免疫功能监测中的应用价值.方法采集48例行肝移植术后患者和30例健康志愿者的外周血样本,采用Immuknow免疫细胞功能测定试剂盒,检测CD4+T细胞ATP值;流式细胞仪检测外周血T淋巴细胞亚群CD3+、CD4+、CD8+细胞的比例.结果正常人、稳定组、感染组与急性排斥组ATP值分别为(331±103)、(364±125)、(182±104)和(479±116) μg/L,急性排斥组明显高于感染组(P＜0.01);稳定组与感染组比较,感染组明显降低(P＜0.01);稳定组与急性排斥组比较差异无统计学意义(P＞0.05).T淋巴细胞亚群检测结果,稳定组CD4+细胞百分比为(33.23±11.34)％,感染组为(25.53±6.89)％,两组比较差异有统计学意义(P＜0.05).结论 CD4+T细胞ATP值以及T淋巴细胞亚群CD4+细胞百分比能较好地反映肝移植患者术后免疫功能.
목적 탐토CD4+T세포삼린산선감(ATP)치급T림파세포아군측정재간이식술후면역공능감측중적응용개치.방법 채집48례행간이식술후환자화30례건강지원자적외주혈양본,채용Immuknow면역세포공능측정시제합,검측CD4+T세포ATP치;류식세포의검측외주혈T림파세포아군CD3+、CD4+、CD8+세포적비례.결과 정상인、은정조、감염조여급성배척조ATP치분별위(331±103)、(364±125)、(182±104)화(479±116) μg/L,급성배척조명현고우감염조(P＜0.01);은정조여감염조비교,감염조명현강저(P＜0.01);은정조여급성배척조비교차이무통계학의의(P＞0.05).T림파세포아군검측결과,은정조CD4+세포백분비위(33.23±11.34)％,감염조위(25.53±6.89)％,량조비교차이유통계학의의(P＜0.05).결론 CD4+T세포ATP치이급T림파세포아군CD4+세포백분비능교호지반영간이식환자술후면역공능.
Objective To investigate the significance of adenosine-triphosphate (ATP) value of CD4+ T cells and T cell subset analysis in monitoring immune function after liver transplantation.Methods Immuknow immune cell function kit was applied to test the ATP value of CD4 + T cells in peripheral blood from 48 patients undergoing liver transplantation and 30 healthy volunteers.Additionally,the frequency of CD3+,CD4+ and CD8+ cell subsets was analyzed by flow cytometry.Results For healthy volunteers,stable recipients,infectious recipients and recipients with acute rejection,the mean ATP levels were (331 ± 103),(364 ± 125),(182 ± 104) and (479 ± 116) μg/L,respectively.The ATP levels in acute rejection patients were higher than stable ones (P ＜ 0.01) and patients with infections (P ＜ 0.01).However,there was no statistically significant difference between stable recipients and recipients with acute rejection (P ＞ 0.05).T cell subsets analysis showed that the CD4+ cell counts were (25.53 ± 6.89) ％ in the infectious patients and (33.23 ± 11.34) ％ in stable group,there was a significantly different in two groups (P ＜ 0.05).Conclusion The ATP levels in CD4+ T cells and T cell subsets analysis can indicate the immune status after liver transplantation to a certain extent.