中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
4期
850-852
,共3页
王文斌%刘三光%刘兵%吕海涛%闫长青
王文斌%劉三光%劉兵%呂海濤%閆長青
왕문빈%류삼광%류병%려해도%염장청
胆管癌%趋化因子受体4%预后
膽管癌%趨化因子受體4%預後
담관암%추화인자수체4%예후
Cholangiocarcinoma%CXC chemokine recetper 4%Prognosis
目的 观察CXC趋化因子受体-4(CXCR4)在胆管癌中的表达,探讨CXCR4与胆管癌临床病理特征及预后的关系.方法 采用免疫组织化学染色及逆转录-聚合酶链反应(RT-PCR)法观察CXCR4在82例胆管癌、7例胆道良性肿瘤、12例非肿瘤胆道上皮组织以及30例新鲜胆管癌组织中的表达.结果 CXCR4蛋白在82例胆管癌、7例胆道良性肿瘤、12例非肿瘤胆道上皮组织中阳性表达率分别为75.6％、28.6％和0.CXCR4蛋白在胆管癌组织中的表达水平明显高于胆道良性肿瘤和非肿瘤胆道上皮组织(P＜0.01).胆管癌组织中CXCR4蛋白表达Ⅱ～Ⅳ期患者均高于Ⅰ期的表达(22.4％比7.3％,P＞0.05).RT-PCR结果显示胆管癌组织中CXCR4mRNA相对表达水平与淋巴结转移和临床分期密切相关,淋巴结转移组和非转移组的相对表达量分别为0.44±0.07和0.23 ±0.03,淋巴结转移组明显高于非转移组(P＜0.05);Ⅱ、Ⅲ期病例CXCR4mRNA的表达水平明显高于Ⅰ期病例(0.43±0.07比0.25 ±0.08,P ＜0.05).结论 CXCR4可能参与了胆管癌的发生,并促进胆管癌的浸润、转移.
目的 觀察CXC趨化因子受體-4(CXCR4)在膽管癌中的錶達,探討CXCR4與膽管癌臨床病理特徵及預後的關繫.方法 採用免疫組織化學染色及逆轉錄-聚閤酶鏈反應(RT-PCR)法觀察CXCR4在82例膽管癌、7例膽道良性腫瘤、12例非腫瘤膽道上皮組織以及30例新鮮膽管癌組織中的錶達.結果 CXCR4蛋白在82例膽管癌、7例膽道良性腫瘤、12例非腫瘤膽道上皮組織中暘性錶達率分彆為75.6％、28.6％和0.CXCR4蛋白在膽管癌組織中的錶達水平明顯高于膽道良性腫瘤和非腫瘤膽道上皮組織(P＜0.01).膽管癌組織中CXCR4蛋白錶達Ⅱ～Ⅳ期患者均高于Ⅰ期的錶達(22.4％比7.3％,P＞0.05).RT-PCR結果顯示膽管癌組織中CXCR4mRNA相對錶達水平與淋巴結轉移和臨床分期密切相關,淋巴結轉移組和非轉移組的相對錶達量分彆為0.44±0.07和0.23 ±0.03,淋巴結轉移組明顯高于非轉移組(P＜0.05);Ⅱ、Ⅲ期病例CXCR4mRNA的錶達水平明顯高于Ⅰ期病例(0.43±0.07比0.25 ±0.08,P ＜0.05).結論 CXCR4可能參與瞭膽管癌的髮生,併促進膽管癌的浸潤、轉移.
목적 관찰CXC추화인자수체-4(CXCR4)재담관암중적표체,탐토CXCR4여담관암림상병리특정급예후적관계.방법 채용면역조직화학염색급역전록-취합매련반응(RT-PCR)법관찰CXCR4재82례담관암、7례담도량성종류、12례비종류담도상피조직이급30례신선담관암조직중적표체.결과 CXCR4단백재82례담관암、7례담도량성종류、12례비종류담도상피조직중양성표체솔분별위75.6％、28.6％화0.CXCR4단백재담관암조직중적표체수평명현고우담도량성종류화비종류담도상피조직(P＜0.01).담관암조직중CXCR4단백표체Ⅱ～Ⅳ기환자균고우Ⅰ기적표체(22.4％비7.3％,P＞0.05).RT-PCR결과현시담관암조직중CXCR4mRNA상대표체수평여림파결전이화림상분기밀절상관,림파결전이조화비전이조적상대표체량분별위0.44±0.07화0.23 ±0.03,림파결전이조명현고우비전이조(P＜0.05);Ⅱ、Ⅲ기병례CXCR4mRNA적표체수평명현고우Ⅰ기병례(0.43±0.07비0.25 ±0.08,P ＜0.05).결론 CXCR4가능삼여료담관암적발생,병촉진담관암적침윤、전이.
Objective To investigate the relationship between CXC chemokine recetper 4 (CXCR4) expression to clinicopathological factors and prognosis in cholangiocarcinoma.Methods The expression of CXCR4 in 82 cases of cholangiocarcinoma,7 cases of benign bile duct neoplasms and 12 cases of non-tumor bile duct epithelium was detected by immunohistochemical staining,and that in 30 cases of cholangiocarcinoma by reverse transcriptase-polymerase chain reaction (RT-PCR).Results The positive expression rate of CXCR4 in 82 cases of cholangiocarcinoma,7 cases of benign bile duct neoplasms,and 12 cases of non-tumor bile duct epithelium was 75.6％,28.6％ and 0,respectively.The expression of CXCR4 in cholangiocarcinoma was significantly higher than that in benign bile duct neoplasms and non-tumor bile duct epithelium (P ＜0.01).The positive rate of CXCR4 was slightly higher in the stage Ⅱ-Ⅳ cases than that in the stage Ⅰ cases (22.4％ vs.7.3％,P ＞0.05).RT-PCR results revealed that the expression of CXCR4 mRNA in stage Ⅱ and Ⅲ cases was higher than that in stage Ⅰ cases (0.43 ± 0.07 vs.0.25 ± 0.08,P ＜ 0.05).The expression of CXCR4 mRNA in the cases with lymph node metastasis was significantly higher than that without lymph node metastasis (0.44 ± 0.07 vs.0.23 ± 0.03,P ＜ 0.05).Conclusion CXCR4 may play a positive role in carcinogenesis,invasion and metastasis of cholangiocarcinoma.
