CAJ | 학술논문

目的探讨微小RNA(miR)-146a在大肠癌及结直肠息肉患者中的表达及意义.方法收集49例大肠癌患者,37例结直肠息肉患者和30例健康志愿者的血清,应用Taqman荧光定量聚合酶链反应(PCR)方法检测miR-146a在3组中的表达及治疗后的水平,酶联免疫吸附试验(ELISA)测定癌胚抗原(CEA)水平.分析miR-146a在3组中表达的差异以及大肠癌不同临床病理特征的组间差异.结果大肠癌组miR-146a水平为38.12±3.56,高于结直肠息肉组和对照组,差异有统计学意义(P＜0.05).在有淋巴结转移的大肠癌患者中miR-146a表达水平(42.39±5.49)明显高于无淋巴结转移的患者(35.33±6.19,P＜0.05),Dukes分期中的C～D期患者中miR-146a表达水平(41.09±7.11)高于A～B期患者(36.17±0.61,P＜0.05),在不同年龄组及性别组的表达水平上差异无统计学意义(P＞0.05).治疗后大肠癌细胞miR-146a表达和CEA结果分别为(33.40±2.98) μg/L和(5.32±0.48) μg/L,均低于治疗前的(38.12±3.56) μg/L和(6.68±0.67) μg/L,差异有统计学意义(P＜0.05).结论 miR-146a在大肠癌患者血清中呈高表达,治疗后明显降低,可能在大肠癌的发生、发展中发挥作用.
목적 탐토미소RNA(miR)-146a재대장암급결직장식육환자중적표체급의의.방법 수집49례대장암환자,37례결직장식육환자화30례건강지원자적혈청,응용Taqman형광정량취합매련반응(PCR)방법검측miR-146a재3조중적표체급치료후적수평,매련면역흡부시험(ELISA)측정암배항원(CEA)수평.분석miR-146a재3조중표체적차이이급대장암불동림상병리특정적조간차이.결과 대장암조miR-146a수평위38.12±3.56,고우결직장식육조화대조조,차이유통계학의의(P＜0.05).재유림파결전이적대장암환자중miR-146a표체수평(42.39±5.49)명현고우무림파결전이적환자(35.33±6.19,P＜0.05),Dukes분기중적C～D기환자중miR-146a표체수평(41.09±7.11)고우A～B기환자(36.17±0.61,P＜0.05),재불동년령조급성별조적표체수평상차이무통계학의의(P＞0.05).치료후대장암세포miR-146a표체화CEA결과분별위(33.40±2.98) μg/L화(5.32±0.48) μg/L,균저우치료전적(38.12±3.56) μg/L화(6.68±0.67) μg/L,차이유통계학의의(P＜0.05).결론 miR-146a재대장암환자혈청중정고표체,치료후명현강저,가능재대장암적발생、발전중발휘작용.
Objective To investigate the expression and significance of microRNA (miR)-146a in colorectal cancer and colorectal polyps in patients.Methods The study was separated three groups as follow:49 colorectal cancer cases of patients; 37 cases of colorectal polyps patients; 30 healthy volunteers.And then Taqman real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) was used to detect the expression of miR-146a in the three groups' blood serum,and the expression of miR-146a after treatment.carcinoembryonic antigen (CEA) was determinate by Enzyme linked immunosorbent assay (ELISA) and different clinicopathological features of colorectal cancer group differences.Results The colorectal cancer miR-146a was 38.12 ± 3.56,higher than that of colorectal polyps group and control group,the difference was significant statistically (P ＜ 0.05).The lymph node metastasis miR-146a expression levels with colorectal cancer were significantly higher than without lymph node metastasis (42.39 ± 5.49 vs.35.33 ± 6.19,P ＜ 0.05),Dukes staging of C-D patients miR-146a expression levels higher than A-B patients (41.09 ±7.11 vs.36.17 ±0.61,P ＜0.05),no significant difference with different age groups and gender groups above.After treatment,the expression of miR-146a and CEA were (33.40 ± 2.98) μg/L and (5.32 ±0.48) μg/L,lower than before treatment (38.12 ±3.56) μg/L and (6.68 ±0.67) μg/L),the difference was significant (P ＜ 0.05).Conclusion miR-146a was highly expressed in the serum of patients with colorectal cancer and decreased significantly after treatment.It might play a role in the incidence of colorectal cancer,colorectal cancer development.