中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
4期
865-867,封3
,共4页
结直肠癌%DNA甲基转移酶3B%DNA甲基化%γ-突触核蛋白
結直腸癌%DNA甲基轉移酶3B%DNA甲基化%γ-突觸覈蛋白
결직장암%DNA갑기전이매3B%DNA갑기화%γ-돌촉핵단백
Colorectal cancer%DNA methyltransferase 3B%DNA methylation%γ-synuclein
目的 观察DNA甲基转移酶3B (DNMT3B)、γ-突触核蛋白(SNCG)在结直肠癌组织中的表达,探讨DNMT3B、SNCG在结直肠癌组织中表达意义及其相关性.方法 采用免疫组织化学染色方法检测72例结直肠癌组织及30例癌旁正常结直肠组织中DNMT3B、SNCG的表达,分析其在结直肠癌组织中的表达与临床病理参数间的关系.结果 DNMT3B在结直肠癌组织中的阳性细胞表达率55.56％ (40/72)明显高于正常结直肠组织黏膜的表达率23.33％ (7/30),两者差异有统计学意义(P＜0.01).DNMT3B的异常表达与结直肠癌患者年龄、性别、肿瘤大小、浸润深度无明显相关(P＞0.05),与组织分化程度、淋巴结转移及TNM分期密切相关(P＜0.01).SNCG在结直肠癌组织中的阳性细胞表达率41.67％ (30/72)明显高于正常结直肠组织黏膜的表达率6.67％(2/30),两者差异有统计学意义(P＜0.01).SNCG的异常表达与结直肠癌患者年龄、性别、肿瘤大小、分化程度无明显相关(P＞0.05),与浸润深度、淋巴结转移及TNM分期密切相关(P＜0.05).结直肠癌组织中DNMT3B表达与SNCG表达两者呈明显负相关(r=-0.321,P＜0.01).结论 DNMT3B和SNCG在结直肠癌组织中异常表达,参与结直肠癌的发生、发展,两者之间密切相关.
目的 觀察DNA甲基轉移酶3B (DNMT3B)、γ-突觸覈蛋白(SNCG)在結直腸癌組織中的錶達,探討DNMT3B、SNCG在結直腸癌組織中錶達意義及其相關性.方法 採用免疫組織化學染色方法檢測72例結直腸癌組織及30例癌徬正常結直腸組織中DNMT3B、SNCG的錶達,分析其在結直腸癌組織中的錶達與臨床病理參數間的關繫.結果 DNMT3B在結直腸癌組織中的暘性細胞錶達率55.56％ (40/72)明顯高于正常結直腸組織黏膜的錶達率23.33％ (7/30),兩者差異有統計學意義(P＜0.01).DNMT3B的異常錶達與結直腸癌患者年齡、性彆、腫瘤大小、浸潤深度無明顯相關(P＞0.05),與組織分化程度、淋巴結轉移及TNM分期密切相關(P＜0.01).SNCG在結直腸癌組織中的暘性細胞錶達率41.67％ (30/72)明顯高于正常結直腸組織黏膜的錶達率6.67％(2/30),兩者差異有統計學意義(P＜0.01).SNCG的異常錶達與結直腸癌患者年齡、性彆、腫瘤大小、分化程度無明顯相關(P＞0.05),與浸潤深度、淋巴結轉移及TNM分期密切相關(P＜0.05).結直腸癌組織中DNMT3B錶達與SNCG錶達兩者呈明顯負相關(r=-0.321,P＜0.01).結論 DNMT3B和SNCG在結直腸癌組織中異常錶達,參與結直腸癌的髮生、髮展,兩者之間密切相關.
목적 관찰DNA갑기전이매3B (DNMT3B)、γ-돌촉핵단백(SNCG)재결직장암조직중적표체,탐토DNMT3B、SNCG재결직장암조직중표체의의급기상관성.방법 채용면역조직화학염색방법검측72례결직장암조직급30례암방정상결직장조직중DNMT3B、SNCG적표체,분석기재결직장암조직중적표체여림상병리삼수간적관계.결과 DNMT3B재결직장암조직중적양성세포표체솔55.56％ (40/72)명현고우정상결직장조직점막적표체솔23.33％ (7/30),량자차이유통계학의의(P＜0.01).DNMT3B적이상표체여결직장암환자년령、성별、종류대소、침윤심도무명현상관(P＞0.05),여조직분화정도、림파결전이급TNM분기밀절상관(P＜0.01).SNCG재결직장암조직중적양성세포표체솔41.67％ (30/72)명현고우정상결직장조직점막적표체솔6.67％(2/30),량자차이유통계학의의(P＜0.01).SNCG적이상표체여결직장암환자년령、성별、종류대소、분화정도무명현상관(P＞0.05),여침윤심도、림파결전이급TNM분기밀절상관(P＜0.05).결직장암조직중DNMT3B표체여SNCG표체량자정명현부상관(r=-0.321,P＜0.01).결론 DNMT3B화SNCG재결직장암조직중이상표체,삼여결직장암적발생、발전,량자지간밀절상관.
Objective To study the expression and significance of DNA methyltransferase 3B (DNMT3B) and γ-synuclein (SNCG) in colorectal cancer and their correlation.Methods The expressions of DNMT3B and SNCG were detected by streptavidin avidin-peroxidase (SP) immunohistochemical method in 72 cases of colorectal cancer and 40 cases of nearby normal colorectal mucosa,and the relationship between the expression of DNMTB and SNCG with the clinicopathological parameters was analyzed.Results The DNMT3B expression rates of positive cell in colorectal cancer and nearby normal colorectal mucosa were 55.56％ (40/72) and 23.33％ (7/30) respectively.The difference was significant between these two groups (P ＜0.01).The over-expression of DNMT3B was correlated with differentiation degree of cancinoma,metastasis of lymph node and TNM stage (P ＜ 0.01),and was not correlated with gender,age,tumor size and depth of infiltration (P ＞ 0.05).The SNCG expression rates of positive cell in colorectal cancer and nearby normal colorectal mucosa were 41.67％ (30/72) and 6.67％ (2/30)respectively.The difference was significant between these two groups (P ＜ 0.01).The over-expression of SNCG was correlated with depth of infiltration,metastasis of lymph node and TNM stage (P ＜0.01),and was not correlated with gender,age,tumor size and degree of cancinoma (P ＞ 0.05).There was a negative correlation between the expression of DNMT3B and SNCG (r =-0.321,P ＜ 0.01).Conclusion DNMT3B and SNCG were abnormally expressed in colorectal cancer,and they may play important roles in the tumorigenesis and progression of colorectal cancer and there is a close relationships between them.
