CAJ | 학술논문

目的探讨颅脑创伤后外周血内皮祖细胞(EPCs)迁移能力和CD147表达的变化及意义.方法收集96例颅脑创伤患者伤后6h～6d外周血进行EPC分离培养,检测细胞迁移能力和CD147表达.结果 EPCs迁移能力在伤后6h～4d高于正常对照组,CD147表达自伤后6h～3d高于正常对照组.EPCs迁移能力与CD147表达呈正相关(rs=0.485,P＜0.05).在伤后12 h和24 h格拉斯哥昏迷评分(GCS) 3～5组的EPCs迁移能力及CD147水平均低于GCS 6 ～8组(P＜0.05).在致伤后7d内死亡的患者,其EPCs迁移能力及CD147水平均低于生存的患者(P＜0.05).预后不良组EPCs迁移能力及CD147水平在伤后6、24 h低于预后良好组(P＜0.05).结论颅脑创伤后EPCs迁移能力与CD147水平密切相关,预后较好具有更高GCS评分的颅脑创伤患者的EPCs具有更好的迁移能力及更高的CD147水平.
목적 탐토로뇌창상후외주혈내피조세포(EPCs)천이능력화CD147표체적변화급의의.방법 수집96례로뇌창상환자상후6h～6d외주혈진행EPC분리배양,검측세포천이능력화CD147표체.결과 EPCs천이능력재상후6h～4d고우정상대조조,CD147표체자상후6h～3d고우정상대조조.EPCs천이능력여CD147표체정정상관(rs=0.485,P＜0.05).재상후12 h화24 h격랍사가혼미평분(GCS) 3～5조적EPCs천이능력급CD147수평균저우GCS 6 ～8조(P＜0.05).재치상후7d내사망적환자,기EPCs천이능력급CD147수평균저우생존적환자(P＜0.05).예후불량조EPCs천이능력급CD147수평재상후6、24 h저우예후량호조(P＜0.05).결론 로뇌창상후EPCs천이능력여CD147수평밀절상관,예후교호구유경고GCS평분적로뇌창상환자적EPCs구유경호적천이능력급경고적CD147수평.
Objective This study examined the migration and expression of CD147 of human endothelial progenitor cells (EPCs) in severe traumatic brain injury (TBI) patients and their association with clinical outcome.Methods Blood samples were obtained from enrolled 96 severe TBI subjects [Glasgow coma score [GCS] ≤8] and healthy donors.After EPCs were cultured,we studied the temporal profile of the migration ability and expression of CD147 over 6 days.Results Comparison of the migration ability and expression of CD147 in TBI patients versus controls showed significant elevation of the migration ability at 6 h and over the 4 day study period and significant elevation of CD147 at 6 h and over the 3 day (P ＜ 0.05).The migration ability and expression of CD147 in patients with GCS 6-8 at first 12 h and 24 h were significantly higher than those with GCS 3-5 (P ＜ 0.05).The migration ability and expression of CD147 appeared to distinguish severe TBI survivors vs.non-survivors within the study,with survivors having significantly higher migration ability and expression of CD147 (P ＜ 0.05).Furthermore,better migration ability and higher expression of CD147 appeared in good outcome groups than in poor outcome groups (P ＜ 0.05).Conclusion The increased migration ability of EPCs was correlated with increased CD147 after TBI.The migration ability and expression of CD147 in EPCs appear to have potential clinical utility in diagnosing TBI.