CAJ | 학술논문

目的观察中胚叶特异性转录子(MEST)在鼠成骨细胞中的表达对细胞迁移及增殖能力的影响.方法取鼠成骨细胞进行培养,利用siRNA干扰片段下调MEST在成骨细胞中的表达,采用Transwell实验检测细胞的迁移能力,细胞计数试剂盒(CCK-8)法及常用增殖指标细胞核增殖抗原(Ki-67)检测细胞的增殖能力.结果 MEST表达下调后,成骨细胞的迁移数目为(10.0±1.9)个,明显低于空载组[(23.5±2.1)个]及空白对照组[(25.2±1.8)个],差异有统计学意义(P＜0.05);同时,CCK-8实验表明成骨细胞的增殖能力明显减弱,Ki-67表达明显降低.结论 MEST表达对成骨细胞具有促迁移和增殖的作用.
목적 관찰중배협특이성전록자(MEST)재서성골세포중적표체대세포천이급증식능력적영향.방법 취서성골세포진행배양,이용siRNA간우편단하조MEST재성골세포중적표체,채용Transwell실험검측세포적천이능력,세포계수시제합(CCK-8)법급상용증식지표세포핵증식항원(Ki-67)검측세포적증식능력.결과 MEST표체하조후,성골세포적천이수목위(10.0±1.9)개,명현저우공재조[(23.5±2.1)개]급공백대조조[(25.2±1.8)개],차이유통계학의의(P＜0.05);동시,CCK-8실험표명성골세포적증식능력명현감약,Ki-67표체명현강저.결론 MEST표체대성골세포구유촉천이화증식적작용.
Objective To investigate the effect of mesoderm specific transcript (MEST) expression on the migration and proliferation of rat osteoblasts.Methods siRNA interference fragments were used to knock down the MEST expression in osteoblasts.Transwell assay was used to detect the migration ability,and cell counting kit-8 (CCK-8) and the detection of proliferation cell nuclear antigen (Ki-67) proliferation index were used to assess the proliferation ability.Results After MEST down-regulation,the number of migratory osteoblasts was (10.0 ± 1.9),significantly less than the mock-vehicle group (23.5 ±2.1) and control group (25.2 ± 1.8),with a statistically significant difference (P ＜ 0.05).Meanwhile,CCK-8 assay revealed that MEST down-regulation significantly reduced the proliferation ability of osteoblasts,and Ki-67 expression was also significantly decreased.Conclusion MEST expression can promote the migration and proliferation of osteoblasts.