CAJ | 학술논문

目的观察辛伐他汀对猪脊髓缺血再灌注损伤(SCII)后热休克蛋白70 (Hsp70)表达的影响.方法 24头健康成年西藏小型猪,雌雄不限,体质量20～30 kg,随机分为假手术组(C组)、缺血再灌注损伤组(I/R组)和辛伐他汀实验组(Sim组),每组8头.I/R组和Sim组采用胸主动脉、锁骨下动脉阻断法造成脊髓缺血再灌注损伤模型(70 min),分离副半奇静脉,远端结扎,近端插管.I/R组在阻断过程中副半奇静脉逆灌注乳酸林格钠液(860 ml/h,1000 ml),Sim组阻断过程中副半奇静脉逆灌辛伐他汀溶液(0.25 mg/kg,1000 ml),术后口服辛伐他汀片剂80 mg/d,30 d.术后采用Tarlov下肢神经功能评分系统评估6、24、48 h神经运动功能.术后30 d,各组分别处死8头实验猪,迅速取出来L2～L5及骶段脊髓,苏木素-伊红(HE)、Nissle染色观察神经元形态学变化,免疫组织化学法检测Hsp70的表达.结果 Sim组术后24、48 h神经功能评分[(3.2±0.3)、(3.6±0.2)分]高于I/R组[(2.2±0.4)、(2.8±0.4)分],差异有统计学意义(P＜0.05).与I/R组比较,Sim组神经元形态学的损伤较轻,Hsp70表达明显增加,其积分吸光度值为296.8046±3.695 1.结论辛伐他汀对猪脊髓缺血再灌注损伤具有保护作用,其机制可能和诱导Hsp70的表达上调有关.
목적 관찰신벌타정대저척수결혈재관주손상(SCII)후열휴극단백70 (Hsp70)표체적영향.방법 24두건강성년서장소형저,자웅불한,체질량20～30 kg,수궤분위가수술조(C조)、결혈재관주손상조(I/R조)화신벌타정실험조(Sim조),매조8두.I/R조화Sim조채용흉주동맥、쇄골하동맥조단법조성척수결혈재관주손상모형(70 min),분리부반기정맥,원단결찰,근단삽관.I/R조재조단과정중부반기정맥역관주유산림격납액(860 ml/h,1000 ml),Sim조조단과정중부반기정맥역관신벌타정용액(0.25 mg/kg,1000 ml),술후구복신벌타정편제80 mg/d,30 d.술후채용Tarlov하지신경공능평분계통평고6、24、48 h신경운동공능.술후30 d,각조분별처사8두실험저,신속취출래L2～L5급저단척수,소목소-이홍(HE)、Nissle염색관찰신경원형태학변화,면역조직화학법검측Hsp70적표체.결과 Sim조술후24、48 h신경공능평분[(3.2±0.3)、(3.6±0.2)분]고우I/R조[(2.2±0.4)、(2.8±0.4)분],차이유통계학의의(P＜0.05).여I/R조비교,Sim조신경원형태학적손상교경,Hsp70표체명현증가,기적분흡광도치위296.8046±3.695 1.결론 신벌타정대저척수결혈재관주손상구유보호작용,기궤제가능화유도Hsp70적표체상조유관.
Objective To investigate the protective effect of simvastatin on spinal cord ischemia/reperfusion injury through the observation of the changes of heat shock protein 70 (Hsp70).Methods 24healthy adult Tibet mini-pigs of either sex,weighted 20-30 kg,were randomly divided into three groups (n =8),including sham operation group (group C),ischemia/reperfusion group (group I/R) and simvastatin experimental group (group Sim).In group I/R and Sim,the models of spinal cord ischemia/reperfusion were established by the occlusion of thoracic aorta and subclavian artery (70 min).The accessory hemiazygos vein was isolated and the distal end was ligated.In group I/R,1000 ml lactated ringer solution were pumped into the vein and the rate was 860 ml/h during the ischemia time.In group Sim,1000 ml simvastatin solution were pumped into the vein and the dose was 0.25 mg/kg during the ischemia time.Feed the swines of the experimental group simvastatin tablets 80 mg/d,orally for 30 days.After the operation,the motor function scores were recorded at 6,24,48 h (see Taylor motor function scoring system).All eight swines of each group were killed at 30 days after reperfusion,taking the L2-L5 and sacral spinal cord as quickly as possible.The hematoxylin and eosin (HE) staining method and the NISSLE staining method were adopted to observe the morphological changes of neurons and the immunohistochemical method was adopted to detect the changes of Hsp70.Results In group Sim,the 24,48 h motor function scores (3.2 ± 0.3,3.6 ± 0.2 respectively) after operation were significantly higher than that in group I/R (2.2 ±0.4,2.8 ± 0.4 respectively) (P ＜ 0.05).Compared with group I/R,the morphology of neurons in group Sim had a slight damage and higher expression changes of Hsp70 can be found in group Sim (P ＜ 0.05).Conclusion Simvastatin has a significant protective effect on the spinal cord ischemia reperfusion injury and the potential mechanism may be related to the up-regulation of Hsp70.