中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
7期
1440-1442
,共3页
卜志勇%郑玲%李安军%涂圣旭%施永彦
蔔誌勇%鄭玲%李安軍%塗聖旭%施永彥
복지용%정령%리안군%도골욱%시영언
脊髓损伤%性别%大鼠%动物实验
脊髓損傷%性彆%大鼠%動物實驗
척수손상%성별%대서%동물실험
Spinal cord injury%Gender difference%Rat%Animal experiment
目的 探讨雌雄大鼠脊髓损伤(SCI)后神经功能恢复的差异性和可能原因.方法 健康SPF级SD大鼠雌雄各30只,按性别分为两组:雄性大鼠组(A组)和雌性大鼠组(B组);改良Allen法在胸7节段平面制作SCI模型.于术后1、2、3、4、7d各组随机处死大鼠2只取损伤处脊髓检测超氧化物歧化酶(SOD)、丙二醇(MDA)和细胞凋亡,术后1d和1、2、3、4周采用改良大鼠胸段脊髓损伤后功能评判标准(BBB评分法)对大鼠进行行为学检查评分,同时各组随机处死1只大鼠取损伤处脊髓冰冻切片,苏木素-伊红(HE)染色显微镜下观察组织结构变化.结果 (1)BBB评分:脊髓损伤后2～4周时,两组大鼠的后肢功能均出现部分恢复,至第4周,B组评分:(8.250±1.658)分,A组为:(5.580±1.443)分,差异有统计学意义(P＜0.01).(2)SOD活性变化:脊髓损伤2d时SOD活性最低,A组:(24.073 ±3.220) U/ml,B组:(42.663±3.940) U/ml;随后均逐渐升高,至7d时,A组SOD活性:(93.556 ±7.138) U/ml,B组:(106.833±5.878) U/ml,在1周内A组均低于B组(P＜0.01).(3)MDA含量变化:脊髓损伤2d内两组MDA含量差异无统计学意义,至第3、4和7天时,A组MDA含量为(28.822±2.804)、(25.106±1.911)、(15.163±3.075) μmol/L;B组分别为:(24.224±2.627)、(18.377±2.416)、(10.959±2.780) μmol/L,差异有统计学意义(P＜0.05).(4)细胞凋亡:在脊髓损伤3d时达高峰,A组:(187.83±18.30)个/视野,B组:(160.67±8.34)个/视野;至7d时,A组:(84.00±10.96)个/视野,B组:(56.00±7.43)个/视野;在3、4、7d时A组细胞凋亡数明显高于B组(P＜0.01).(5)病理切片观察,损伤脊髓组织的炎症浸润、空洞形成、白质留存、瘢痕形成等方面差异有统计学意义(P＜0.05),B组优于A组.结论 雌性大鼠SCI后运动功能的恢复优于雄性大鼠;其原因可能是雌性大鼠体内相对高水平的雌激素存在,通过抗氧化作用和抑制细胞凋亡,减轻了继发性损伤.
目的 探討雌雄大鼠脊髓損傷(SCI)後神經功能恢複的差異性和可能原因.方法 健康SPF級SD大鼠雌雄各30隻,按性彆分為兩組:雄性大鼠組(A組)和雌性大鼠組(B組);改良Allen法在胸7節段平麵製作SCI模型.于術後1、2、3、4、7d各組隨機處死大鼠2隻取損傷處脊髓檢測超氧化物歧化酶(SOD)、丙二醇(MDA)和細胞凋亡,術後1d和1、2、3、4週採用改良大鼠胸段脊髓損傷後功能評判標準(BBB評分法)對大鼠進行行為學檢查評分,同時各組隨機處死1隻大鼠取損傷處脊髓冰凍切片,囌木素-伊紅(HE)染色顯微鏡下觀察組織結構變化.結果 (1)BBB評分:脊髓損傷後2～4週時,兩組大鼠的後肢功能均齣現部分恢複,至第4週,B組評分:(8.250±1.658)分,A組為:(5.580±1.443)分,差異有統計學意義(P＜0.01).(2)SOD活性變化:脊髓損傷2d時SOD活性最低,A組:(24.073 ±3.220) U/ml,B組:(42.663±3.940) U/ml;隨後均逐漸升高,至7d時,A組SOD活性:(93.556 ±7.138) U/ml,B組:(106.833±5.878) U/ml,在1週內A組均低于B組(P＜0.01).(3)MDA含量變化:脊髓損傷2d內兩組MDA含量差異無統計學意義,至第3、4和7天時,A組MDA含量為(28.822±2.804)、(25.106±1.911)、(15.163±3.075) μmol/L;B組分彆為:(24.224±2.627)、(18.377±2.416)、(10.959±2.780) μmol/L,差異有統計學意義(P＜0.05).(4)細胞凋亡:在脊髓損傷3d時達高峰,A組:(187.83±18.30)箇/視野,B組:(160.67±8.34)箇/視野;至7d時,A組:(84.00±10.96)箇/視野,B組:(56.00±7.43)箇/視野;在3、4、7d時A組細胞凋亡數明顯高于B組(P＜0.01).(5)病理切片觀察,損傷脊髓組織的炎癥浸潤、空洞形成、白質留存、瘢痕形成等方麵差異有統計學意義(P＜0.05),B組優于A組.結論 雌性大鼠SCI後運動功能的恢複優于雄性大鼠;其原因可能是雌性大鼠體內相對高水平的雌激素存在,通過抗氧化作用和抑製細胞凋亡,減輕瞭繼髮性損傷.
목적 탐토자웅대서척수손상(SCI)후신경공능회복적차이성화가능원인.방법 건강SPF급SD대서자웅각30지,안성별분위량조:웅성대서조(A조)화자성대서조(B조);개량Allen법재흉7절단평면제작SCI모형.우술후1、2、3、4、7d각조수궤처사대서2지취손상처척수검측초양화물기화매(SOD)、병이순(MDA)화세포조망,술후1d화1、2、3、4주채용개량대서흉단척수손상후공능평판표준(BBB평분법)대대서진행행위학검사평분,동시각조수궤처사1지대서취손상처척수빙동절편,소목소-이홍(HE)염색현미경하관찰조직결구변화.결과 (1)BBB평분:척수손상후2～4주시,량조대서적후지공능균출현부분회복,지제4주,B조평분:(8.250±1.658)분,A조위:(5.580±1.443)분,차이유통계학의의(P＜0.01).(2)SOD활성변화:척수손상2d시SOD활성최저,A조:(24.073 ±3.220) U/ml,B조:(42.663±3.940) U/ml;수후균축점승고,지7d시,A조SOD활성:(93.556 ±7.138) U/ml,B조:(106.833±5.878) U/ml,재1주내A조균저우B조(P＜0.01).(3)MDA함량변화:척수손상2d내량조MDA함량차이무통계학의의,지제3、4화7천시,A조MDA함량위(28.822±2.804)、(25.106±1.911)、(15.163±3.075) μmol/L;B조분별위:(24.224±2.627)、(18.377±2.416)、(10.959±2.780) μmol/L,차이유통계학의의(P＜0.05).(4)세포조망:재척수손상3d시체고봉,A조:(187.83±18.30)개/시야,B조:(160.67±8.34)개/시야;지7d시,A조:(84.00±10.96)개/시야,B조:(56.00±7.43)개/시야;재3、4、7d시A조세포조망수명현고우B조(P＜0.01).(5)병리절편관찰,손상척수조직적염증침윤、공동형성、백질류존、반흔형성등방면차이유통계학의의(P＜0.05),B조우우A조.결론 자성대서SCI후운동공능적회복우우웅성대서;기원인가능시자성대서체내상대고수평적자격소존재,통과항양화작용화억제세포조망,감경료계발성손상.
Objective To explore the difference of the neural function and some reasons in male and female rats after spinal cord injury (SCI).Methods By the striking of the spinal cord at the level of T7 with Allen's device,acute SCI was induced in anesthetized rats.Sixty SCI model rats were divided into 2 groups by gender:group A (male group) and group B (female group),each with 30 SD rats.The changes of superoxide dismutase (SOD),malondialdehyde (MDA) and cell apotosis in the lesion site were observed in 1,2,3,4 and 7 days after SCI.The motor functional changes were evaluated by BBB scale in 1 day and 1,2,3,4 weeks after SCI.And at the same time point,the injuried spinal cord was made into frozen section,histomorphology change was observed by hematoxylin and eosin (HE) staining.Results The motor capacity of hind limb was some obvious recovery in every group in 2-4 w after modeling.The BBB scores were significantly higher in group B (8.25 ±1.658) than in group A (5.58 ±1.443) after 4 w (P＜0.01).The SOD activity was the minimum in both group A [(28.822±2.804) U/ml] and group B [(106.833 ±5.878) U/ml] in 2 d.To 7 d,it were (93.556 ±7.138) U/ml in group A and (106.833 ±5.878) U/ml in group.It was higher in group B than group A in 7 d (P ＜ 0.01).The evel of MDA was no difference in 2 d after SCI modeling.To 3,4 and 7 d,it was (28.822 ±2.804),(25.106 ± 1.911),(15.163 ±3.075) μnol/L in group A,and (24.224 ± 2.627),(18.377 ± 2.416),(10.959 ± 2.780) μmol/L in group A (P ＜ 0.05).The biggest number of apoptotic cells was (187.83 ± 18.30) cell/view in group A and (160.67 ± 8.34) cell/view in group B after 3 d.It was (84.00 ± 10.96) cell/view in group A and (56.00 ± 7.43) cell/view in group B in 7 d.It was less in goup B than in group A in 2,3,4,7 d (P ＜ 0.01).Group B was better than group A in pathological changes of spinal cord injury,incloud inflammatory,cavitation,white matter retained,scar formation.Condusion The recovery of motor capacity of hind limb is better after SCI in female rats than in male rats; the reason may be that there are more estrogen in female rat than in male rat,because estrogens can reduce oxygen free radicals and adip-peroxidation,decrease apoptosis of nerve cell in the injury site of spinal cord.
