CAJ | 학술논문

目的利用维甲酸灌胃孕鼠诱导的大鼠子鼠脊髓脊膜膨出动物模型,建立一种动物实验方法,观察壳聚糖明胶薄膜在修补子鼠脊髓脊膜膨出的效果.方法利用维甲酸灌胃孕SD大鼠获得脊髓脊膜膨出的子鼠动物模型,在孕中期(孕18d)利用经腹经子宫开放手术,以壳聚糖明胶薄膜作为组织工程材料修补脊髓脊膜膨出皮肤缺损.在孕21 d取胎,观察手术成功率,并以未经手术修补的子鼠为对照,观察组织病理学改变.结果通过建立动物模型,用44只可利用的子鼠顺利进行了28例修补,修补后存活13只,其中获得了10只修补满意的子鼠.观察到了修补手术后局部的组织改变.以未经修补的子鼠为对照,通过测定免疫组织化学染色切片中的吸光度值,发现神经微管蛋白-β-Ⅲ在子鼠膀胱表达显著高于对照组,且和普通子鼠比较差异无统计学意义(P＞0.05).结论壳聚糖-明胶薄膜作为组织工程材料可应用在该手术模型中,对脊髓脊膜膨出子鼠膀胱神经发育有改善作用.
목적 이용유갑산관위잉서유도적대서자서척수척막팽출동물모형,건립일충동물실험방법,관찰각취당명효박막재수보자서척수척막팽출적효과.방법 이용유갑산관위잉SD대서획득척수척막팽출적자서동물모형,재잉중기(잉18d)이용경복경자궁개방수술,이각취당명효박막작위조직공정재료수보척수척막팽출피부결손.재잉21 d취태,관찰수술성공솔,병이미경수술수보적자서위대조,관찰조직병이학개변.결과 통과건립동물모형,용44지가이용적자서순리진행료28례수보,수보후존활13지,기중획득료10지수보만의적자서.관찰도료수보수술후국부적조직개변.이미경수보적자서위대조,통과측정면역조직화학염색절편중적흡광도치,발현신경미관단백-β-Ⅲ재자서방광표체현저고우대조조,차화보통자서비교차이무통계학의의(P＞0.05).결론 각취당-명효박막작위조직공정재료가응용재해수술모형중,대척수척막팽출자서방광신경발육유개선작용.
Objective To establish an animal experimental method for investigating the effect of fetal myelomeningocele (MMC) repair with chitosan gelatin membrane,and then observe the bladder protective effect of fetal surgery.Methods Fetal myelomeningocele rats were obtained by feeding pregnant rats with retinoic acid.Open abdominal fetal surgery was performed,and repair of MMC using chitosan gelatin membrane as tissue engineering materials.Fetal rats after repair were harvested bofore dilivery.By observing the success rate of surgery and pathological changes,data were analyzed compare to MMC fetus in groups.Results 44 fetuses underwent repairment,and the use of chitosan gelatin membrane patch in fetal surgery was successful in 10 fetuses.Local tissue changes after the repair of MMC.According to the optical density value in immunochemistry staining,the expression of nerve tubulin-β-Ⅲ in the fetal bladder was significantly higher in operation group as compare with MMC control group,and it had no difference to normal control.Condusion Our preliminary study confirmed that chitosan gelatin membrane as tissue engineering materials in the MMC fetal surgery is feasible,and it showed the protection effect to fetal spine and bladder.