中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
7期
1445-1447
,共3页
周立亚%周立文%李明强%叶习红%叶繁%王雪松
週立亞%週立文%李明彊%葉習紅%葉繁%王雪鬆
주립아%주립문%리명강%협습홍%협번%왕설송
基因转染%血红素加氧酶-1%腺病毒%脊髓缺血%再灌注损伤
基因轉染%血紅素加氧酶-1%腺病毒%脊髓缺血%再灌註損傷
기인전염%혈홍소가양매-1%선병독%척수결혈%재관주손상
Gene transfection%Heme oxygenase-1%Adenovirus%Spinal cord ischemia%Reperfusion injury
目的 观察腺病毒介导血红素加氧酶-1(HO-1)基因对大鼠脊髓缺血再灌注损伤的保护作用.方法 雄性SD大鼠分成假手术组(A组)、缺血再灌注组(B组)、AdV-rHO-1处理组(C组).A组为对照组,B、C组阻断腹主动脉30 min后,暴露大鼠脊髓分别注入AdV-EGFP、AdV-rHO-1病毒载体.各组于缺血再灌注12h、1、2、3、7d测定后肢神经功能评分、病理学、HO-1、HO-1 mRNA的表达和凋亡细胞.结果 C组后肢神经功能评分(2.83±0.75)在缺血再灌注2d后明显好于B组(1.50±0.55) (P ＜0.01).C组(35.85±6.59)HO-1表达量较A、B组明显增多(P＜0.01),C组(1.83±0.28) HO-1的mRNA表达量较A组和B组明显升高(P＜0.01).结论 腺病毒介导的HO-1基因能有效转染缺血再灌注脊髓组织,对缺血再灌注的脊髓有保护作用.
目的 觀察腺病毒介導血紅素加氧酶-1(HO-1)基因對大鼠脊髓缺血再灌註損傷的保護作用.方法 雄性SD大鼠分成假手術組(A組)、缺血再灌註組(B組)、AdV-rHO-1處理組(C組).A組為對照組,B、C組阻斷腹主動脈30 min後,暴露大鼠脊髓分彆註入AdV-EGFP、AdV-rHO-1病毒載體.各組于缺血再灌註12h、1、2、3、7d測定後肢神經功能評分、病理學、HO-1、HO-1 mRNA的錶達和凋亡細胞.結果 C組後肢神經功能評分(2.83±0.75)在缺血再灌註2d後明顯好于B組(1.50±0.55) (P ＜0.01).C組(35.85±6.59)HO-1錶達量較A、B組明顯增多(P＜0.01),C組(1.83±0.28) HO-1的mRNA錶達量較A組和B組明顯升高(P＜0.01).結論 腺病毒介導的HO-1基因能有效轉染缺血再灌註脊髓組織,對缺血再灌註的脊髓有保護作用.
목적 관찰선병독개도혈홍소가양매-1(HO-1)기인대대서척수결혈재관주손상적보호작용.방법 웅성SD대서분성가수술조(A조)、결혈재관주조(B조)、AdV-rHO-1처리조(C조).A조위대조조,B、C조조단복주동맥30 min후,폭로대서척수분별주입AdV-EGFP、AdV-rHO-1병독재체.각조우결혈재관주12h、1、2、3、7d측정후지신경공능평분、병이학、HO-1、HO-1 mRNA적표체화조망세포.결과 C조후지신경공능평분(2.83±0.75)재결혈재관주2d후명현호우B조(1.50±0.55) (P ＜0.01).C조(35.85±6.59)HO-1표체량교A、B조명현증다(P＜0.01),C조(1.83±0.28) HO-1적mRNA표체량교A조화B조명현승고(P＜0.01).결론 선병독개도적HO-1기인능유효전염결혈재관주척수조직,대결혈재관주적척수유보호작용.
Objective To study the protective effect of adenovirus-mediated heme oxygenase-1 (HO-1) transfection on the spinal cord ischemia-reperfusion injury (IRI) in rats.Methods SD rats were randomly divided into sham operation group (group A),ischemia reperfusion group (group B) and AdV-rHO-1 group (group C),The rats in groups B and C were given spinal cord ischemia for 30 min.Physiological saline,AdV-EGFP,and AdV-rHO-1 were injected in groups A,B and C,respectively.Hind legs nerve function grading,apoptotic cells,immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) were used to evaluate the degree of spinal cord injury.Results The hind legs nerve function grading (2.83 ± 0.75) in group C was obviously better than in group B (1.5 ± 0.55) after reperfusion for 2 days (P ＜ 0.01).The histopathologic changes in group C were obviously milder than those in group B.Immunohistochemistry and RT-PCR indicated that transgene was already expressed in group C.The level of HO-1 in group C (35.85 ±6.59) was higher than that in group A (7.24 ± 1.15) or group B (8.21 ± 3.05) (P ＜ 0.01).Conclusion Adenovirus-mediated gene transfection of rHO-1 has significantly protective effect on spinal cord IRI.
