CAJ | 학술논문

目的探讨黏着斑激酶(FAK)及细胞核增殖抗原(Ki-67)在骨肉瘤组织中的表达及临床意义.方法免疫组织化学法检测35例骨肉瘤、18例骨样骨瘤和8例反应性新生骨组织中FAK和Ki-67的表达,并结合临床资料进行相关分析.结果 FAK表达的染色积分在骨肉瘤、骨样骨瘤和反应性新生骨组织中分别为5.41±1.27、1.02±0.14和0.32±0.19 (P＜0.05);在EnnekingⅠ、Ⅱ和Ⅲ期患者中分别为2.15±1.01、4.54±2.14和6.83±2.43(P ＜0.01);在年龄小于25岁患者和大于25岁患者中分别为3.75 ±2.13和4.17 ±2.53(P ＞0.05);在男性患者和女性患者中分别为4.35±2.38和4.47±2.62(P＞0.05);在生存期小于2年和大于2年的患者中分别为6.94±2.17和2.65±1.87(P＜0.01).Ki-67标记指数(Ki-67LI)在Enneking Ⅰ、Ⅱ和Ⅲ期患者中分别为8.76±3.68、18.25 ±3.31和25.87±4.36(P ＜0.01);在年龄小于25岁患者和大于25岁患者中分别为10.72±2.35和9.14±2.62 (P＞0.05);在男性患者和女性患者中分别为11.67±4.28和12.15±3.65(P ＞0.05);在生存期小于2年和大于2年的患者中分别为22.84±2.17和12.47±3.68(P ＜0.01).在FAK表达为阳性和阴性的骨肉瘤中,Ki-67LI分别为19.74±3.23和11.63±2.85(P ＜0.05).结论 FAK和Ki-67的表达水平与骨肉瘤的分期和生存期密切相关,FAK与Ki-67之间也密切相关.
목적 탐토점착반격매(FAK)급세포핵증식항원(Ki-67)재골육류조직중적표체급림상의의.방법 면역조직화학법검측35례골육류、18례골양골류화8례반응성신생골조직중FAK화Ki-67적표체,병결합림상자료진행상관분석.결과 FAK표체적염색적분재골육류、골양골류화반응성신생골조직중분별위5.41±1.27、1.02±0.14화0.32±0.19 (P＜0.05);재EnnekingⅠ、Ⅱ화Ⅲ기환자중분별위2.15±1.01、4.54±2.14화6.83±2.43(P ＜0.01);재년령소우25세환자화대우25세환자중분별위3.75 ±2.13화4.17 ±2.53(P ＞0.05);재남성환자화녀성환자중분별위4.35±2.38화4.47±2.62(P＞0.05);재생존기소우2년화대우2년적환자중분별위6.94±2.17화2.65±1.87(P＜0.01).Ki-67표기지수(Ki-67LI)재Enneking Ⅰ、Ⅱ화Ⅲ기환자중분별위8.76±3.68、18.25 ±3.31화25.87±4.36(P ＜0.01);재년령소우25세환자화대우25세환자중분별위10.72±2.35화9.14±2.62 (P＞0.05);재남성환자화녀성환자중분별위11.67±4.28화12.15±3.65(P ＞0.05);재생존기소우2년화대우2년적환자중분별위22.84±2.17화12.47±3.68(P ＜0.01).재FAK표체위양성화음성적골육류중,Ki-67LI분별위19.74±3.23화11.63±2.85(P ＜0.05).결론 FAK화Ki-67적표체수평여골육류적분기화생존기밀절상관,FAK여Ki-67지간야밀절상관.
Objective To investigate the expression of focal adhesion kinase (FAK) and proliferating cell nuclear antigen (PCNA) in osteosarcoma.Methods The expression of FAK and PCNA (Ki-67)was detected by immunochemistry in 35 osteosarcoma samples,18 osteoid osteoma samples and 8 newly formed responsive bone tissue samples.Results The staining scores of FAK,evaluated by immunochemistry,were 5.41 ± 1.27,1.02 ±0.14 and 0.32 ±0.19 in osteosarcoma,osteoid osteoma and newly formed responsive bone tissue samples (P ＜ 0.05),2.15 ± 1.01,4.54 ± 2.14 and 6.83 ± 2.43 in Enneking stage Ⅰ,Ⅱ and Ⅲ (P ＜0.01),3.75 ±2.13 and 4.17 ±2.53 in patients younger or older than 25 yearsold (P ＞0.05),4.35 ±2.38 and 4.47 ±2.62 in male and female patients (P ＞0.05),and 6.94 ±2.17 and 2.65 ± 1.87 in patients with survival less than 2 years or more than 2 years (P ＜0.01),respectively.The Ki-67 labelling index (Ki-67LI) was 8.76 ±3.68,18.25 ±3.31 and 25.87 ±4.36 in Enneking stage Ⅰ,Ⅱ and Ⅲ (P ＜0.01),10.72 ±2.35 and 9.14 ±2.62 in patients younger or older than 25 years old (P ＞ 0.05),11.67 ± 4.28 and 12.15 ± 3.65 in male and female patients (P ＞ 0.05),and 22.84 ± 2.17 and 12.47 ± 3.68 in patients with survival less than 2 years or more than 2 years (P ＜ 0.01),respectively.In FAK positive or negative osteosarcoma samples,Ki-67LI was 19.74 ± 3.23 or 11.63 ± 2.85 (P ＜0.05),respectively.Conclusion The expression levels of FAK and Ki-67 were highly correlated with the staging and prognosis of osteosarcoma.Moreover,FAK and Ki-67 might be related with each other.