中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
7期
1482-1484
,共3页
乳腺癌%耐药%紫杉醇%肿瘤干细胞
乳腺癌%耐藥%紫杉醇%腫瘤榦細胞
유선암%내약%자삼순%종류간세포
Breast cancer%Drug resistance%Taxol%Cancer stem cell
目的 观察肿瘤干细胞(CSC)在人乳腺癌细胞株MCF-7对紫杉醇(Taxol)产生获得性耐药中的作用.方法 采用低浓度加量(起始浓度为0.005 mg/L)持续诱导法诱导产生人乳腺癌耐Taxol细胞株MCF-7/T=ol,噻唑蓝(MTT)法检测MCF-7/Taxol对Taxol的敏感性.实时定量聚合酶链反应(Real-time PCR)检测三磷酸腺苷结合转运蛋白G超家族成员2(ABCG2)和性别决定区Y框蛋白-2(SOx-2)的表达.结果 亲本株和耐药株细胞对Taxol的半数抑制浓度(IC5o)值分别为0.05 mg/L和4.2 mg/L,耐药指数为84.0.20 mg/L的As2O3降低了耐药株对Taxol的耐药性,逆转倍数为3.82,相对逆转效率为74.7％.耐药株细胞ABCG2和SOX-2的表达显著升高(P＜0.01).As2O3处理后,ABCG2和SOX-2的表达显著下降(P＜0.05).结论 CSC有可能是人乳腺癌细胞株MCF-7对Taxol产生耐药的机制之一.
目的 觀察腫瘤榦細胞(CSC)在人乳腺癌細胞株MCF-7對紫杉醇(Taxol)產生穫得性耐藥中的作用.方法 採用低濃度加量(起始濃度為0.005 mg/L)持續誘導法誘導產生人乳腺癌耐Taxol細胞株MCF-7/T=ol,噻唑藍(MTT)法檢測MCF-7/Taxol對Taxol的敏感性.實時定量聚閤酶鏈反應(Real-time PCR)檢測三燐痠腺苷結閤轉運蛋白G超傢族成員2(ABCG2)和性彆決定區Y框蛋白-2(SOx-2)的錶達.結果 親本株和耐藥株細胞對Taxol的半數抑製濃度(IC5o)值分彆為0.05 mg/L和4.2 mg/L,耐藥指數為84.0.20 mg/L的As2O3降低瞭耐藥株對Taxol的耐藥性,逆轉倍數為3.82,相對逆轉效率為74.7％.耐藥株細胞ABCG2和SOX-2的錶達顯著升高(P＜0.01).As2O3處理後,ABCG2和SOX-2的錶達顯著下降(P＜0.05).結論 CSC有可能是人乳腺癌細胞株MCF-7對Taxol產生耐藥的機製之一.
목적 관찰종류간세포(CSC)재인유선암세포주MCF-7대자삼순(Taxol)산생획득성내약중적작용.방법 채용저농도가량(기시농도위0.005 mg/L)지속유도법유도산생인유선암내Taxol세포주MCF-7/T=ol,새서람(MTT)법검측MCF-7/Taxol대Taxol적민감성.실시정량취합매련반응(Real-time PCR)검측삼린산선감결합전운단백G초가족성원2(ABCG2)화성별결정구Y광단백-2(SOx-2)적표체.결과 친본주화내약주세포대Taxol적반수억제농도(IC5o)치분별위0.05 mg/L화4.2 mg/L,내약지수위84.0.20 mg/L적As2O3강저료내약주대Taxol적내약성,역전배수위3.82,상대역전효솔위74.7％.내약주세포ABCG2화SOX-2적표체현저승고(P＜0.01).As2O3처리후,ABCG2화SOX-2적표체현저하강(P＜0.05).결론 CSC유가능시인유선암세포주MCF-7대Taxol산생내약적궤제지일.
Objective To investigate the role of cancer stem cells (CSC) in the acquired resist ance to Taxol of human breast cancer cell line MCF-7.Methods Taxol-resistant MCF-7 (MCF-7/Taxol) was established in vitro by exposure to low concentration (0.005 mg/L) and subsequently the gradually increased dose of Taxol.The proliferation and sensitivity to Taxol of MCF-7/Taxol before and after application of As2O3 were tested using methyl thiazol tetrazolium (MTT) assay.Real-time quantitative polymerase chain reaction (Real-time PCR) was employed to investigate the expression of ATP-binding cassette subfamily G member 2 (ABCG2) and sex determining region Y-box 2 (SOX-2) in wild type MCF-7 and MCF-7/Taxol cells.Results In the presence of Taxol (0.5 mg/L),the growth rate of MCF-7/ Taxol was significantly higher than the wild type MCF-7 cells (P ＜ 0.01).It was also demonstrated that MCF-7/Taxol ceils treated by Taxol developed similar proliferation to wild type MCF-7 cells in the absence of Taxol (P ＞ 0.05).The 50％ inhibitory dose (IC50) of wild type MCF-7 and MCF-7/Taxol cells to Taxol was about 0.05 mg/L and 4.2 mg/L respectively,and the resistance index was about 84.The resistance of MCF-7/Taxol ceils to Taxol was reduced after treatment of As2O3 (0.20 mg/L) with reversed multiples being 3.82 and relative reversed efficiency being 74.7％ respectively.MCF-7/Taxol cells showed elevated expression of ABCG2 and SOX-2 (P ＜0.01) in comparison with wild type MCF-7 cells.After treatment of As2O3,MCF-7/Taxol cells developed decreased levels of ABCG2 and SOX-2 (P ＜ 0.05).Conclusion CSC is a possible mechanism accounting for the acquired resistance of MCF-7/Taxol cells to Taxol.
