CAJ | 학술논문

目的探讨吲哚胺-2,3双加氧酶(IDO)在脓毒症患者免疫炎症进展过程中的表达及其临床意义.方法选取90例脓毒症患者,按照疾病严重程度分为脓毒症组(32例)、严重脓毒症组(30例)及脓毒性休克组(28例);选择30例健康人作为对照组.采用流式细胞仪检测CD4+、CD8+、CD4+ CD25+调节性T细胞(Treg)的水平,计算CD4 +/CD8+百分比.采用酶联免疫吸附试验(ELISA)检测白细胞介素(IL)-6、IL-10、干扰素-γ(INF-γ)、IDO的表达水平.结果对照组、脓毒症组、严重脓毒症组、脓毒性休克组IL-6水平分别为(45.23±8.0)、(86.84±7.83)、(142.01±11.11)、(206.92±17.79) ng/L,各组间比较差异有统计学意义(P＜0.05);IL-10水平分别为(9.34±3.58)、(20.17±4.04)、(39.04±4.29)、(64.96±4.41)n#L,各组间比较差异有统计学意义(P＜0.05);IL-6/IL-10分别为6.45±1.17、4.53±1.03、3.86±0.83、3.20 ±0.62,各组间比较差异有统计学意义(P ＜0.05);INF-y分别为(2.58±1.36)、(5.68±0.59)、(17.29±2.46)、(31.28±4.49) ng/L,各组间比较差异有统计学意义(P＜0.05);IDO分别为(13.41±1.80)、(22.88±3.61)、(34.38±2.2)、(45.35±4.29) ng/L,各组间比较差异有统计学意义(P＜0.05);CD4+分别为(37.35±1.79)％、(29.38±1.53)％、(26.80±0.97)％、(21.22±1.44)％,各组间比较差异有统计学意义(P＜0.05);CD8+分别为(19.61±0.73)％、(20.33±1.01)％、(23.1±0.98)％、(25.63±0.52)％,各组间比较差异有统计学意义(P＜0.05);CD4 +/CD8+分别为1.91±0.15、1.42±0.07、1.16±0.08、0.83±0.07,各组间比较差异有统计学意义(P＜0.05);Treg分别为(0.62 ±0.11)％、(1.96±0.87)％、(3.12±1.02)％、(4.35±1.23)％,各组间比较差异有统计学意义(P＜0.05).结论 IDO在免疫炎性反应中发挥重要作用,有助于评估脓毒症的严重程度及预后.
목적 탐토신타알-2,3쌍가양매(IDO)재농독증환자면역염증진전과정중적표체급기림상의의.방법 선취90례농독증환자,안조질병엄중정도분위농독증조(32례)、엄중농독증조(30례)급농독성휴극조(28례);선택30례건강인작위대조조.채용류식세포의검측CD4+、CD8+、CD4+ CD25+조절성T세포(Treg)적수평,계산CD4 +/CD8+백분비.채용매련면역흡부시험(ELISA)검측백세포개소(IL)-6、IL-10、간우소-γ(INF-γ)、IDO적표체수평.결과 대조조、농독증조、엄중농독증조、농독성휴극조IL-6수평분별위(45.23±8.0)、(86.84±7.83)、(142.01±11.11)、(206.92±17.79) ng/L,각조간비교차이유통계학의의(P＜0.05);IL-10수평분별위(9.34±3.58)、(20.17±4.04)、(39.04±4.29)、(64.96±4.41)n#L,각조간비교차이유통계학의의(P＜0.05);IL-6/IL-10분별위6.45±1.17、4.53±1.03、3.86±0.83、3.20 ±0.62,각조간비교차이유통계학의의(P ＜0.05);INF-y분별위(2.58±1.36)、(5.68±0.59)、(17.29±2.46)、(31.28±4.49) ng/L,각조간비교차이유통계학의의(P＜0.05);IDO분별위(13.41±1.80)、(22.88±3.61)、(34.38±2.2)、(45.35±4.29) ng/L,각조간비교차이유통계학의의(P＜0.05);CD4+분별위(37.35±1.79)％、(29.38±1.53)％、(26.80±0.97)％、(21.22±1.44)％,각조간비교차이유통계학의의(P＜0.05);CD8+분별위(19.61±0.73)％、(20.33±1.01)％、(23.1±0.98)％、(25.63±0.52)％,각조간비교차이유통계학의의(P＜0.05);CD4 +/CD8+분별위1.91±0.15、1.42±0.07、1.16±0.08、0.83±0.07,각조간비교차이유통계학의의(P＜0.05);Treg분별위(0.62 ±0.11)％、(1.96±0.87)％、(3.12±1.02)％、(4.35±1.23)％,각조간비교차이유통계학의의(P＜0.05).결론 IDO재면역염성반응중발휘중요작용,유조우평고농독증적엄중정도급예후.
Objective To investigate the expression of indoleamine 2,3-dioxygenase (IDO) in patients with sepsis during the immune inflammation progression,and the role of IDO in immune inflammation in sepsis.Methods A total of 90 cases of sepsis were collected from June 2011 to October 2013 during their stay in our hospital,and divided into three groups according to their disease severity:sepsis group (n =32),severe sepsis group (n =30) and septic shock group (n =28).Thirty healthy individuals served as control group.The percentages of CD4 +,CD8 +,and CD4 + CD25 + regulatory T cells (Tregs)were detected by flow cytometry.The CD4+/CD8 + ratio was calculated.Interleukin (IL)-6,IL-10,INF-γ,and IDO concentrations were detected by enzyme-linked immunosorbent assay (ELISA).Results With the increased inflammatory response,IL-6,IL-10,INF-γ,and IDO concentrations were gradually increased,and the percentate of CD8 + Tregs was gradually increased with the difference being statistically significant among all groups (P ＜ 0.01 for all).IL-6 level was decreased,and concentrations of IL-10 and CD4 + and the CD4 +/CD8 + ratio were also gradually reduced with the difference being statistically significant among all groups (P ＜ 0.01 for all).Conclusion IDO may play an important role in immune inflammatory response,which may help to assess the severity and prognosis of sepsis.