CAJ | 학술논문

目的观察胃癌患者血清中骨桥蛋白(OPN)的表达水平及其对肿瘤转移的作用.方法收集156例胃癌患者的病历资料及胃癌组织,采用酶联免疫吸附试验(EHSA)法检测胃癌患者和以健康正常人为对照组的血清OPN的含量,以胃癌患者中OPN的含量中位数来分组,以高于中位数浓度组为高浓度组,低于中位数浓度组为低浓度组,研究术前OPN浓度与术后胃癌复发及转移之间的关系;采用Westem blot法检测高浓度组癌细胞转移患者和低浓度组癌细胞未转移患者的胃癌组织及对照组胃黏膜组织中OPN及Tat作用蛋白30(TIP30)的表达量,比较各组之间的差异.结果胃癌患者OPN含量为75.66 μg/L,明显高于对照组的OPN含量30.45 μg/L,差异有统计学意义(P＜0.01);高浓度组患者2年的复发率和转移率分别为45.3％和64.1％,明显高于低浓度组的25.6％和11.5％,差异有统计学意义(P＜0.01);Western bl结果显示,高浓度组和低浓度组患者的胃癌组织中的OPN表达率明显高于对照组,且高浓度组OPN的表达量高于低浓度组;而正常组的TIP30明显高于高浓度组和低浓度组,且高浓度组患者的胃癌组织中TIP30的表达几乎缺失.结论胃癌患者血清OPN的表达水平升高,与胃癌细胞的转移密切相关.
목적 관찰위암환자혈청중골교단백(OPN)적표체수평급기대종류전이적작용.방법 수집156례위암환자적병력자료급위암조직,채용매련면역흡부시험(EHSA)법검측위암환자화이건강정상인위대조조적혈청OPN적함량,이위암환자중OPN적함량중위수래분조,이고우중위수농도조위고농도조,저우중위수농도조위저농도조,연구술전OPN농도여술후위암복발급전이지간적관계;채용Westem blot법검측고농도조암세포전이환자화저농도조암세포미전이환자적위암조직급대조조위점막조직중OPN급Tat작용단백30(TIP30)적표체량,비교각조지간적차이.결과 위암환자OPN함량위75.66 μg/L,명현고우대조조적OPN함량30.45 μg/L,차이유통계학의의(P＜0.01);고농도조환자2년적복발솔화전이솔분별위45.3％화64.1％,명현고우저농도조적25.6％화11.5％,차이유통계학의의(P＜0.01);Western bl결과현시,고농도조화저농도조환자적위암조직중적OPN표체솔명현고우대조조,차고농도조OPN적표체량고우저농도조;이정상조적TIP30명현고우고농도조화저농도조,차고농도조환자적위암조직중TIP30적표체궤호결실.결론 위암환자혈청OPN적표체수평승고,여위암세포적전이밀절상관.
Objective To study the expression of serum osteopontin (OPN) in gastric cancer patients and its effect on tumor metastasis.Methods To choose 156 cases of patients with gastric cancer and gastric cancer tissues,using enzyme linked immunosorbent assay (ELISA) method to detect patients with gastric cancer and in normal human health in the control group,the content of serum osteopontin in gastric cancer patients with median content of OPN in groups,with higher than the median concentration for high concentration group,lower than the median concentration to low concentration group,the preoperative OPN concentration and the relationship between the postoperative gastric cancer recurrence and metastasis,compared the difference between high and low concentration group; Using western blot method to detect cancer metastasis in the high concentration group and low concentration group of cancer metastasis in patients with gastric cancer group and health control group not TIP30 and OPN expression in gastric mucosa tissue volume,compare the difference between groups.Results OPN levels in patients with gastric cancer was 75.66 μg/L,significantly higher than the control group of OPN content 30.45 μg/L,the difference was statistically significant (P ＜0.01) ; High concentration group of patients 2 years of metastasis and recurrence rate were 45.3％ and 64.1％,significantly higher than the 25.6％ and 11.5％ of the low concentration group,the difference was statistically significant (P ＜ 0.01) ; Western blotting results show that in the high and low concentration group of patients,the OPN expression rate in gastric cancer tissue was obviously higher than that of control group,and high concentration group patients with the expression of OPN quantity is higher than the low concentration group of patients; However,the expression of TIP30 in the normal group significantly higher than both the high and lowconcentration group of patients,and high concentration group of patients gastric cancer tissue expression of TIP30 almost absent.Conclusion The expression of OPN levels serum of gastric cancer patients,is closely related to the transfer of gastric cancer cells.