CAJ | 학술논문

目的探讨胃癌组织中微小RNA(miR)-433的表达变化及其临床意义.方法采用荧光定量聚合酶链反应(FQ-PCR)检测80例胃癌患者手术切除癌组织及配对癌旁组织标本中miR-433的表达水平.结果 miR-433在胃癌组织中的相对表达水平(△Ct为9.73±2.96)显著低于其配对癌旁组织中的表达(△Ct为12.25±2.07),差异有统计学意义(P＜0.05).胃癌组织中miR-433的表达水平与分化程度、临床TNM分期、肿瘤大小及淋巴结转移密切相关(P＜0.05),而与年龄和性别无相关(P＞0.05).结论胃癌组织中miR-433的低表达可能与胃癌的发生、发展、侵袭及转移相关.
목적 탐토위암조직중미소RNA(miR)-433적표체변화급기림상의의.방법 채용형광정량취합매련반응(FQ-PCR)검측80례위암환자수술절제암조직급배대암방조직표본중miR-433적표체수평.결과 miR-433재위암조직중적상대표체수평(△Ct위9.73±2.96)현저저우기배대암방조직중적표체(△Ct위12.25±2.07),차이유통계학의의(P＜0.05).위암조직중miR-433적표체수평여분화정도、림상TNM분기、종류대소급림파결전이밀절상관(P＜0.05),이여년령화성별무상관(P＞0.05).결론 위암조직중miR-433적저표체가능여위암적발생、발전、침습급전이상관.
Objective To investigate the expression of micmRNA (miR)-433 in gastric carcinoma and the clinical significance.Methods Eighty pairs of human gastric carcinoma tissue samples were obtained from patients who underwent surgical resection.The level of miR-433 in 80 caes of gastric carcinomas and the paired adjacent normal tissues was quantified by real-time fluorescent quantitative polymerase chain reaction (FQ-PCR),and the relationship between miR-433 and clinicopathological features in gastric carcinoma was analyzed.Results The expression of miR-433 in gastric carcinoma tissue (△Ct:9.73 ±2.96) was significantly decreased as compared with the paired adjacent normal tissues (△Ct:12.25 ±2.07) (P ＜ 0.05).The evaluated level of miR-433 was associated with TNM classification,tumor size,lymph node metastases and local invasion in gastric carcinoma,but not with sex,age,and differentiation.Conclusion These finding suggested miR0433 may play important roles in the development,progression,invasion and metastasis of gastric carcnima.