CAJ | 학술논문

目的探讨CD4+ CD25+叉头框蛋白p3(Fox#)+调节性T细胞在结直肠癌中抑制肿瘤免疫的机制.方法收集结直肠癌组织48例,息肉组织22例,正常组织21例,采用免疫组织化学法检测Foxp3+调节性T细胞及白细胞介素(IL)-10阳性肿瘤细胞在上述组织中的含量;逆转录-聚合酶链反应(RT-PCR)法检测Foxp3及信号转导与转录激活因子(STAT3) mRNA在不同组织中的基因表达,同时采用,检验分析上述指标与肿瘤临床病理特征之间的关系.结果 Foxp3及IL-10在结直肠癌组织中阳性细胞表达率分别为45.8％和68.8％,较息肉组织(13.6％和31.8％)及正常组织(9.5％和23.8％)显著升高(P＜0.01);肿瘤组织中Foxp3及STAT3 mRNA的相对表达量分别为0.644±0.059、0.343±0.036,较息肉组织(0.322±0.020、0.212±0.028)及正常组织(0.309±0.016、0.202±0.021)明显升高(P＜0.01);结直肠癌组织中Foxp3与STAT3基因表达量呈正相关(r =0.526,P＜0.05),Foxp3与IL-10蛋白表达呈正相关(r=0.314,P＜0.05);肿瘤组织中Foxp3的表达量与组织学分级、淋巴结转移、TNM分期明显相关(P＜0.05),与年龄、性别无明显相关(P＞0.05).结论 CD4+ CD25+ Foxp3+调节性T细胞通过Foxp3及其他相关抑制性细胞因子的表达抑制肿瘤免疫,进而引起肿瘤免疫逃逸,从而促进肿瘤的发生发展.
목적 탐토CD4+ CD25+차두광단백p3(Fox#)+조절성T세포재결직장암중억제종류면역적궤제.방법 수집결직장암조직48례,식육조직22례,정상조직21례,채용면역조직화학법검측Foxp3+조절성T세포급백세포개소(IL)-10양성종류세포재상술조직중적함량;역전록-취합매련반응(RT-PCR)법검측Foxp3급신호전도여전록격활인자(STAT3) mRNA재불동조직중적기인표체,동시채용,검험분석상술지표여종류림상병리특정지간적관계.결과 Foxp3급IL-10재결직장암조직중양성세포표체솔분별위45.8％화68.8％,교식육조직(13.6％화31.8％)급정상조직(9.5％화23.8％)현저승고(P＜0.01);종류조직중Foxp3급STAT3 mRNA적상대표체량분별위0.644±0.059、0.343±0.036,교식육조직(0.322±0.020、0.212±0.028)급정상조직(0.309±0.016、0.202±0.021)명현승고(P＜0.01);결직장암조직중Foxp3여STAT3기인표체량정정상관(r =0.526,P＜0.05),Foxp3여IL-10단백표체정정상관(r=0.314,P＜0.05);종류조직중Foxp3적표체량여조직학분급、림파결전이、TNM분기명현상관(P＜0.05),여년령、성별무명현상관(P＞0.05).결론 CD4+ CD25+ Foxp3+조절성T세포통과Foxp3급기타상관억제성세포인자적표체억제종류면역,진이인기종류면역도일,종이촉진종류적발생발전.
Objective To study the mechanism of CD4 + CD25 + forkhead box P3 (Foxp3) + regulatory T cells inhibiting the tumor immunity in colorectal cancer.Methods Thirty-two cases of colorectal carcinoma,21 cases of polypus,and 18 cases of normal tissues were collected.T he immunohistochemistry was used to measure the number of Foxp3 + regulatory T cells and interleukin (IL)-10 positive cells in above tissues,and reverse transcription-polymerase chain reaction (RT-PCR) was done to dectect the expression of Foxp3 and signal transducers and activators of transcription 3 (STAT3) mRNA in different tissues.The relationship between the above indexes and clinicopathological features was analyzed.Results The expression rate of positive cells of Foxp3 and IL-10 in colorectal cancer was respectively 45.8％ and 68.8％,significantly higher than in the polypus tissues (13.6％ and 1.8％) and normal tissues (9.5％and 23.8％) (P ＜0.01 for all).The relative expression amount of Foxp3 and STAT3 mRNA in tumor tissue was respectively 0.644 ± 0.059 and 0.343 ± 0.036,which was significantly increased as compared with polypus (0.322 ±0.020 and 0.212 ±0.028) and normal tissue (0.309 ±0.016 and 0.202 ±0.021)(P ＜0.01 for all).The Foxp3 mRNA expression in colorectal cancer tissues was positively correlated with STAT3 (r =0.526.P ＜ 0.05).The Foxp3 protein expression wass positively correlated with IL-10 (r =0.314,P ＜ 0.05).The expression of Foxp3 in cancer patients was visibley correlated with the histological grade,lymph node metastasis and TNM stage (P ＜ 0.05),and there was no visible correlation with age and gender (P ＞ 0.05).Conclusion CD4 + CD25 + Foxp3 + regulatory T cells inhibit tumor immunity and induce tumor immune escape through the expression of Foxp3 and some other related inhibitory cytokines,and finally promote the tumor progression.