中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
7期
1609-1612
,共4页
郭欣%吴志宇%陈春悠%王东海
郭訢%吳誌宇%陳春悠%王東海
곽흔%오지우%진춘유%왕동해
Th9细胞%白细胞介素-9%喉鳞状细胞癌
Th9細胞%白細胞介素-9%喉鱗狀細胞癌
Th9세포%백세포개소-9%후린상세포암
T helper cells 9%Interleukin-9%Laryngeal squamous cell carcinoma
目的 检测喉鳞状细胞癌患者外周血及癌组织中Th9细胞及白细胞介素(IL)-9的表达水平,探讨其与喉鳞状细胞癌患者临床病理特征的关系.方法 选择66例初治的喉鳞状细胞癌患者和40例健康志愿者.采用流式细胞仪检测外周血Th9细胞水平,酶联免疫吸附法(ELISA)检测血清中IL-9表达水平.应用免疫组织化学染色分析癌旁组织及癌组织中IL-9阳性细胞的浸润程度.分析喉鳞状细胞癌患者外周血Th9细胞、血清中IL-9水平及癌组织中IL-9阳性细胞浸润程度与各临床病理参数间的关系.结果 喉鳞状细胞癌患者外周血中Th9细胞及IL-9水平分别为(2.64±0.24)％和(89.40 ±6.66) ng/L,显著高于健康对照组[(1.07±0.11)％,t=12.63,P＜0.01;(26.80±3.76) ng/L,t=14.37,P＜0.01],Ⅲ～Ⅳ期患者的Th9细胞比例及IL-9水平明显高于Ⅰ～Ⅱ期患者[(3.21±0.22)％比(2.07±0.19)％,t=8.24,P＜0.01;(104.30±6.07) ng/L比(72.90±5.37) ng/L,t=8.91,P＜0.01].喉鳞状细胞癌患者癌组织中IL-9阳性细胞浸润率为84.37％(27/32),而对应癌旁组织为21.87％(7/32),差异有统计学意义(x2=25.098,P＜0.01).外周血Th9细胞和IL-9的表达水平与喉鳞状细胞癌的临床分期、肿瘤分化程度、区域淋巴结转移明显相关(P＜0.05),与年龄、性别、原发灶部位无相关(P＞0.05).癌组织中IL-9阳性细胞浸润程度与临床分期、区域淋巴结转移明显相关(P＜0.05),与其他临床病理特征无相关(P＞0.05).结论 喉鳞状细胞癌患者外周血Th9细胞比例及血浆中IL-9水平显著升高,且与临床分期等病理指标明显相关,提示Th9细胞及IL-9参与喉鳞状细胞癌的发生发展.
目的 檢測喉鱗狀細胞癌患者外週血及癌組織中Th9細胞及白細胞介素(IL)-9的錶達水平,探討其與喉鱗狀細胞癌患者臨床病理特徵的關繫.方法 選擇66例初治的喉鱗狀細胞癌患者和40例健康誌願者.採用流式細胞儀檢測外週血Th9細胞水平,酶聯免疫吸附法(ELISA)檢測血清中IL-9錶達水平.應用免疫組織化學染色分析癌徬組織及癌組織中IL-9暘性細胞的浸潤程度.分析喉鱗狀細胞癌患者外週血Th9細胞、血清中IL-9水平及癌組織中IL-9暘性細胞浸潤程度與各臨床病理參數間的關繫.結果 喉鱗狀細胞癌患者外週血中Th9細胞及IL-9水平分彆為(2.64±0.24)％和(89.40 ±6.66) ng/L,顯著高于健康對照組[(1.07±0.11)％,t=12.63,P＜0.01;(26.80±3.76) ng/L,t=14.37,P＜0.01],Ⅲ～Ⅳ期患者的Th9細胞比例及IL-9水平明顯高于Ⅰ～Ⅱ期患者[(3.21±0.22)％比(2.07±0.19)％,t=8.24,P＜0.01;(104.30±6.07) ng/L比(72.90±5.37) ng/L,t=8.91,P＜0.01].喉鱗狀細胞癌患者癌組織中IL-9暘性細胞浸潤率為84.37％(27/32),而對應癌徬組織為21.87％(7/32),差異有統計學意義(x2=25.098,P＜0.01).外週血Th9細胞和IL-9的錶達水平與喉鱗狀細胞癌的臨床分期、腫瘤分化程度、區域淋巴結轉移明顯相關(P＜0.05),與年齡、性彆、原髮竈部位無相關(P＞0.05).癌組織中IL-9暘性細胞浸潤程度與臨床分期、區域淋巴結轉移明顯相關(P＜0.05),與其他臨床病理特徵無相關(P＞0.05).結論 喉鱗狀細胞癌患者外週血Th9細胞比例及血漿中IL-9水平顯著升高,且與臨床分期等病理指標明顯相關,提示Th9細胞及IL-9參與喉鱗狀細胞癌的髮生髮展.
목적 검측후린상세포암환자외주혈급암조직중Th9세포급백세포개소(IL)-9적표체수평,탐토기여후린상세포암환자림상병리특정적관계.방법 선택66례초치적후린상세포암환자화40례건강지원자.채용류식세포의검측외주혈Th9세포수평,매련면역흡부법(ELISA)검측혈청중IL-9표체수평.응용면역조직화학염색분석암방조직급암조직중IL-9양성세포적침윤정도.분석후린상세포암환자외주혈Th9세포、혈청중IL-9수평급암조직중IL-9양성세포침윤정도여각림상병리삼수간적관계.결과 후린상세포암환자외주혈중Th9세포급IL-9수평분별위(2.64±0.24)％화(89.40 ±6.66) ng/L,현저고우건강대조조[(1.07±0.11)％,t=12.63,P＜0.01;(26.80±3.76) ng/L,t=14.37,P＜0.01],Ⅲ～Ⅳ기환자적Th9세포비례급IL-9수평명현고우Ⅰ～Ⅱ기환자[(3.21±0.22)％비(2.07±0.19)％,t=8.24,P＜0.01;(104.30±6.07) ng/L비(72.90±5.37) ng/L,t=8.91,P＜0.01].후린상세포암환자암조직중IL-9양성세포침윤솔위84.37％(27/32),이대응암방조직위21.87％(7/32),차이유통계학의의(x2=25.098,P＜0.01).외주혈Th9세포화IL-9적표체수평여후린상세포암적림상분기、종류분화정도、구역림파결전이명현상관(P＜0.05),여년령、성별、원발조부위무상관(P＞0.05).암조직중IL-9양성세포침윤정도여림상분기、구역림파결전이명현상관(P＜0.05),여기타림상병리특정무상관(P＞0.05).결론 후린상세포암환자외주혈Th9세포비례급혈장중IL-9수평현저승고,차여림상분기등병리지표명현상관,제시Th9세포급IL-9삼여후린상세포암적발생발전.
Objective To detect the expression of T helper cells 9 (Th9) in the peripheral blood,interleukin (IL)-9 level in the serum and the IL-9 in the tumor tissues of patients with laryngeal squamous cell carcinoma (LSCC),and analyze the relationship between Th9/IL-9 and clinical characteristics and their possible roles in the immunological pathogenesis of LSCC.Methods Sixty-six patients with LSCC and 40 healthy controls were included.Th9 cells in the peripheral blood were detected by flow cytometry.The serum IL-9 level was measured by enzyme-linked immunosorbent assay (ELISA).Immunohistochemistry (IHC) was used to determine the IL-9 expression in paraffin-embedded tissues of patients with LSCC.The relationship between Th9/IL-9 and clinical characteristics was analyzed.Results The level of Th9 cells in peripheral blood and IL-9 levels in serum of patients with LSCC were (2.64 ± 0.24) ％ and (89.40 ± 6.66) ng/L,respectively,significantly higher than those of healthy controls [(1.07 ± 0.11)％,t =12.63,P ＜0.01 ; (26.80 ±3.76) ng/L,t =14.37,P ＜0.01].Patients with Ⅲ-Ⅳ stage had higher Th9 cells and IL-9 levels than those with Ⅰ-Ⅱ stage [(3.21 ± 0.22) ％ vs.(2.07 ± 0.19) ％,t =8.24,P ＜0.01 ; (104.30 ±6.07) ng/L vs.(72.90 ±5.37) ng/L,t =8.91,P ＜0.01].Compared with peritumor tissues,intratumor tissues showed higher expression of IL-9 + cells (84.37％ vs.21.87％,x2 =25.098,P ＜ 0.01).The levels of Th9 cells in peripheral blood and IL-9 in serum were associated with tumor stage,tumor differentiation and regional lymph node metastasis (P ＜ 0.05),while the IL-9 + cells in tumor tissues were associated with tumor stage and regional lymph node metastasis (P ＜ 0.05).Conclusion There are higher quantification of Th9 cells and IL-9 in the peripheral blood and intratumor tissues in patients with LSCC,and they have positive correlation with the clinical stage.Th9 cells and IL-9 may contribute to the disease progression and prognosis of LSCC.
