中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
8期
1648-1649
,共2页
程剑剑%于丹%吴纪珍%李静静%刘豹
程劍劍%于丹%吳紀珍%李靜靜%劉豹
정검검%우단%오기진%리정정%류표
特发性肺间质纤维化%肺泡灌洗液%微小RNA%表达差异
特髮性肺間質纖維化%肺泡灌洗液%微小RNA%錶達差異
특발성폐간질섬유화%폐포관세액%미소RNA%표체차이
Idiopathic pulmonary fibrosis%Bronchoalveolar lavage fluid%MicroRNA%Expression differences
目的 在老年特发性肺间质纤维化(IPF)患者肺泡灌洗液游离囊泡(Exosome)中筛选出表达差异的微小RNA(miRNA).方法 收集10例老年IPF患者及10例健康志愿者的肺泡灌洗液并分离Exosome.进行miRNA分离,分离后获得的miRNA采用高通量miRNA表达差异定量检测阵列进行检测,检测结果采用GeneCopoiea在线工具进行分析.结果 高通量miRNA表达差异定量检测阵列显示,与健康对照组比较,在肺泡灌洗液的Exosome中miR-125b、miR-128、miR-21、miR-100、miR-140-3p、miR-374b与健康对照组比较,表达呈上调趋势,分别增高了5.42、9.93、7.62、5.86、7.80、4.16倍,let-7d、miR-30a、miR-27b和miR-103呈下调趋势,分别下降了2.41、2.87、3.27、3.45倍,差异有统计学意义(P＜0.05).结论 老年IPF患者肺泡灌洗液Exosome中存在miRNA表达差异.
目的 在老年特髮性肺間質纖維化(IPF)患者肺泡灌洗液遊離囊泡(Exosome)中篩選齣錶達差異的微小RNA(miRNA).方法 收集10例老年IPF患者及10例健康誌願者的肺泡灌洗液併分離Exosome.進行miRNA分離,分離後穫得的miRNA採用高通量miRNA錶達差異定量檢測陣列進行檢測,檢測結果採用GeneCopoiea在線工具進行分析.結果 高通量miRNA錶達差異定量檢測陣列顯示,與健康對照組比較,在肺泡灌洗液的Exosome中miR-125b、miR-128、miR-21、miR-100、miR-140-3p、miR-374b與健康對照組比較,錶達呈上調趨勢,分彆增高瞭5.42、9.93、7.62、5.86、7.80、4.16倍,let-7d、miR-30a、miR-27b和miR-103呈下調趨勢,分彆下降瞭2.41、2.87、3.27、3.45倍,差異有統計學意義(P＜0.05).結論 老年IPF患者肺泡灌洗液Exosome中存在miRNA錶達差異.
목적 재노년특발성폐간질섬유화(IPF)환자폐포관세액유리낭포(Exosome)중사선출표체차이적미소RNA(miRNA).방법 수집10례노년IPF환자급10례건강지원자적폐포관세액병분리Exosome.진행miRNA분리,분리후획득적miRNA채용고통량miRNA표체차이정량검측진렬진행검측,검측결과채용GeneCopoiea재선공구진행분석.결과 고통량miRNA표체차이정량검측진렬현시,여건강대조조비교,재폐포관세액적Exosome중miR-125b、miR-128、miR-21、miR-100、miR-140-3p、miR-374b여건강대조조비교,표체정상조추세,분별증고료5.42、9.93、7.62、5.86、7.80、4.16배,let-7d、miR-30a、miR-27b화miR-103정하조추세,분별하강료2.41、2.87、3.27、3.45배,차이유통계학의의(P＜0.05).결론 노년IPF환자폐포관세액Exosome중존재miRNA표체차이.
Objective In oder to select disease-related biomarkers,the differentially expressed microRNAs (miRNAs) from bronchoalveolar lavage fluid (BLAF) exosomes in elderly patients with idiopathic pulmonary fibrosis (IPF) patients were screened.Methods BLAF exosomes were collected from 10 cases of IPF and 10 healthy controls.The miRNA expression was checked by high-throughput quantitative detection of miRNA expression array,and the results were analyzed using online tools GeneCopoiea.Results miR-125b,miR-128,miR-21,miR-100,miR-140-3p and miR-374b in BALF exosomes from IPF patients were checked using high-throughput quantitative detection of miRNA expression array,and the expression of these miRNAs was upregulated respectively by 5.42,9.93,7.62,5.86,7.80 and 4.16 folds compared to the controls,and that of let-7d,miR-30a,miR-27b and miR-103 was downregulated respectively by 2.41,2.87,3.27 and 3.45 folds.Conclusion There is differential expression of miRNA in BLAF exosomes from IPF patients.
