中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
8期
1657-1658
,共2页
崔广晖%崔明威%李宇航%梁有光%李玮浩%郭海周%赵松
崔廣暉%崔明威%李宇航%樑有光%李瑋浩%郭海週%趙鬆
최엄휘%최명위%리우항%량유광%리위호%곽해주%조송
鳞癌%长链非编码RNA%HOTAIR%生物学
鱗癌%長鏈非編碼RNA%HOTAIR%生物學
린암%장련비편마RNA%HOTAIR%생물학
Squamous carcinoma%Long non-coding RNA%HOTAIR%Biology
目的 观察长链非编码RNA HOTAIR(LncRNA HOTAIR)对食管鳞癌细胞EC9706恶性生物学行为的影响.方法 应用小干扰RNA(siRNA)干扰LncRNA HOTAIR表达,分别以噻唑蓝(MTT)法、流式细胞术、Transwell侵袭实验检测LncRNA HOTAIR对EC9706细胞增殖、凋亡及侵袭的影响.结果 LncRNA HOTAIR转染72 h后,EC9706细胞生长抑制率为(45.35±3.25)%;转染24 h后,细胞凋亡率为(37.80±2.56)%;转染72 h后,侵袭抑制率为(51.6±4.8)%,与对照组比较,差异均有统计学意义(P<0.01).结论 LncRNA HOTAIR可能与食管鳞癌细胞的增殖、凋亡及侵袭等生物学行为密切相关.
目的 觀察長鏈非編碼RNA HOTAIR(LncRNA HOTAIR)對食管鱗癌細胞EC9706噁性生物學行為的影響.方法 應用小榦擾RNA(siRNA)榦擾LncRNA HOTAIR錶達,分彆以噻唑藍(MTT)法、流式細胞術、Transwell侵襲實驗檢測LncRNA HOTAIR對EC9706細胞增殖、凋亡及侵襲的影響.結果 LncRNA HOTAIR轉染72 h後,EC9706細胞生長抑製率為(45.35±3.25)%;轉染24 h後,細胞凋亡率為(37.80±2.56)%;轉染72 h後,侵襲抑製率為(51.6±4.8)%,與對照組比較,差異均有統計學意義(P<0.01).結論 LncRNA HOTAIR可能與食管鱗癌細胞的增殖、凋亡及侵襲等生物學行為密切相關.
목적 관찰장련비편마RNA HOTAIR(LncRNA HOTAIR)대식관린암세포EC9706악성생물학행위적영향.방법 응용소간우RNA(siRNA)간우LncRNA HOTAIR표체,분별이새서람(MTT)법、류식세포술、Transwell침습실험검측LncRNA HOTAIR대EC9706세포증식、조망급침습적영향.결과 LncRNA HOTAIR전염72 h후,EC9706세포생장억제솔위(45.35±3.25)%;전염24 h후,세포조망솔위(37.80±2.56)%;전염72 h후,침습억제솔위(51.6±4.8)%,여대조조비교,차이균유통계학의의(P<0.01).결론 LncRNA HOTAIR가능여식관린암세포적증식、조망급침습등생물학행위밀절상관.
Objective To explore the effects of long non-coding RNA (LncRNA) HOTAIR on malignant biological behaviors of esophageal carcinoma EC9706 cells.Methods LncRNA HOTAIR siRNA was transfected into the EC9706 cells.Methyl thiazol tetrazolium (MTT) assay,flow cytometry and Transwell chambers were used to examine the cell proliferation,apoptosis and the invasion ability,respectively.Results LncRNA HOTAIR siRNA significantly inhibited the cell proliferation,the growth inhibition rate (IR) was (45.35 ± 3.25) %,the invasion inhibiting rate was (51.6 ± 4.8) % for 72 h after transfection (P< 0.01); LncRNA HOTAIR siRNA promoted the cell apoptosis,the apoptosis rate was (37.80 ± 2.56) % for 24 h (P < 0.01).Conclusion LncRNA HOTAIR may play an important role in esophageal cancer cell proliferation,invasion and apoptosis,and can serve as an effective target for cancer gene therapy.