中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
8期
1668-1670
,共3页
小鼠%心移植%模型%品系
小鼠%心移植%模型%品繫
소서%심이식%모형%품계
Mice%Cardiac transplantation%Models%Strains
目的 建立小鼠腹部同种异体心脏移植模型,并且探讨不同品系小鼠间移植手术的难易程度以及存活时间的差异.方法 BALB/c和CBA雄性小鼠各40只,C57BL/6j雄性小鼠80只,分为同品系间(BALB/c→BALB/c;CBA→CBA;C57 BL/6j→C57 BL/6j)和不同品系间(BALB/c→C57BL/6j;C57BL/6j→BALB/c;C57BL/6j→CBA;CBA→C57 BL/6j)采用Ono术式进行小鼠腹部异位心脏移植,术后观察移植心存活时间并进行病理检查.结果 手术成功率达88.6％.平均取心时间4.8 min,同品系间血管吻合时间分别为(28.70±3.56) min(BALB/c→BALB/c)、(20.70±4.22) min(CBA→CBA)和(27.90±2.51) min(C57BL/6j→C57BL/6j).而不同品系间血管吻合时间分别为(28.00±2.71) min(BALB/c→C57BL/6j)、(22.10±4.33) min(C57BL/6j→CBA)、(29.90±3.14) min(C57 BL/6j→BALB/c)和(30.33 ± 23.00) min(CBA→C57BL/6j).同品系间移植心平均存活时间均超过100d,而不同品系间分别为(7.10±0.74) d(C57BL/6j→BALB/c)、(7.40±0.82) d(BALB/c→C57 BL/6j)、(8.90±1.10) d(C57BL/6j→CBA)和(8.50±0.85) d(CBA→C57BL/6j).同品系间移植心在术后7d的病理检查显示心肌结构良好.而不同品系间移植心在排异后发现大量淋巴细胞浸润,尤其在BALB/c和C57BL/6j互为移植中.结论 建立小鼠心脏移植模型需要掌握显微外科技巧.同品系间心移植中以CBA→CBA相对简单,可作为练习用.C57BL/6j→BALB/c的免疫反应强烈,而C57BL/6j→CBA免疫反应相对弱,因此可用于不同的免疫抑制药物研究.
目的 建立小鼠腹部同種異體心髒移植模型,併且探討不同品繫小鼠間移植手術的難易程度以及存活時間的差異.方法 BALB/c和CBA雄性小鼠各40隻,C57BL/6j雄性小鼠80隻,分為同品繫間(BALB/c→BALB/c;CBA→CBA;C57 BL/6j→C57 BL/6j)和不同品繫間(BALB/c→C57BL/6j;C57BL/6j→BALB/c;C57BL/6j→CBA;CBA→C57 BL/6j)採用Ono術式進行小鼠腹部異位心髒移植,術後觀察移植心存活時間併進行病理檢查.結果 手術成功率達88.6％.平均取心時間4.8 min,同品繫間血管吻閤時間分彆為(28.70±3.56) min(BALB/c→BALB/c)、(20.70±4.22) min(CBA→CBA)和(27.90±2.51) min(C57BL/6j→C57BL/6j).而不同品繫間血管吻閤時間分彆為(28.00±2.71) min(BALB/c→C57BL/6j)、(22.10±4.33) min(C57BL/6j→CBA)、(29.90±3.14) min(C57 BL/6j→BALB/c)和(30.33 ± 23.00) min(CBA→C57BL/6j).同品繫間移植心平均存活時間均超過100d,而不同品繫間分彆為(7.10±0.74) d(C57BL/6j→BALB/c)、(7.40±0.82) d(BALB/c→C57 BL/6j)、(8.90±1.10) d(C57BL/6j→CBA)和(8.50±0.85) d(CBA→C57BL/6j).同品繫間移植心在術後7d的病理檢查顯示心肌結構良好.而不同品繫間移植心在排異後髮現大量淋巴細胞浸潤,尤其在BALB/c和C57BL/6j互為移植中.結論 建立小鼠心髒移植模型需要掌握顯微外科技巧.同品繫間心移植中以CBA→CBA相對簡單,可作為練習用.C57BL/6j→BALB/c的免疫反應彊烈,而C57BL/6j→CBA免疫反應相對弱,因此可用于不同的免疫抑製藥物研究.
목적 건립소서복부동충이체심장이식모형,병차탐토불동품계소서간이식수술적난역정도이급존활시간적차이.방법 BALB/c화CBA웅성소서각40지,C57BL/6j웅성소서80지,분위동품계간(BALB/c→BALB/c;CBA→CBA;C57 BL/6j→C57 BL/6j)화불동품계간(BALB/c→C57BL/6j;C57BL/6j→BALB/c;C57BL/6j→CBA;CBA→C57 BL/6j)채용Ono술식진행소서복부이위심장이식,술후관찰이식심존활시간병진행병리검사.결과 수술성공솔체88.6％.평균취심시간4.8 min,동품계간혈관문합시간분별위(28.70±3.56) min(BALB/c→BALB/c)、(20.70±4.22) min(CBA→CBA)화(27.90±2.51) min(C57BL/6j→C57BL/6j).이불동품계간혈관문합시간분별위(28.00±2.71) min(BALB/c→C57BL/6j)、(22.10±4.33) min(C57BL/6j→CBA)、(29.90±3.14) min(C57 BL/6j→BALB/c)화(30.33 ± 23.00) min(CBA→C57BL/6j).동품계간이식심평균존활시간균초과100d,이불동품계간분별위(7.10±0.74) d(C57BL/6j→BALB/c)、(7.40±0.82) d(BALB/c→C57 BL/6j)、(8.90±1.10) d(C57BL/6j→CBA)화(8.50±0.85) d(CBA→C57BL/6j).동품계간이식심재술후7d적병리검사현시심기결구량호.이불동품계간이식심재배이후발현대량림파세포침윤,우기재BALB/c화C57BL/6j호위이식중.결론 건립소서심장이식모형수요장악현미외과기교.동품계간심이식중이CBA→CBA상대간단,가작위연습용.C57BL/6j→BALB/c적면역반응강렬,이C57BL/6j→CBA면역반응상대약,인차가용우불동적면역억제약물연구.
Objective To establish the mice heterotopic cardiac transplantation as well as to observe the different graft reaction between different strains.Methods According to the surgical procedures of Ono's pattern in mice cardiac heterotopic transplantation model,we did allograft cardiac transplantation using same (BALB/c→BALB/c ; CBA→CBA; C57BL/6j→C57BL/6j) or different (BALB/c→C57BL/6j ; C57BL/6j→BALB/c; C57BL/6j→CBA; CBA→C57BL/6j)strains male mice.Survival time and pathological examination were observed after transplantation Results The operative survival was 88.6％ (62/70).Mean donor graft harvest time of was 4.8 min,mean anatomic time was (28.70 ± 3.56) min (BALB/c→BALB/c),(20.70±4.22) min (CBA→CBA) and (27.90±2.51) min (C57BL/6j→C57BL/6j) in same strains while (28.00 ± 2.71) min (BALB/c→C57BL/6j),(22.10 ± 4.33) min (C57BL/6j→CBA),(29.90±3.14) min (C57BL/6j→BALB/c) and (30.33 ±23.00) min (CBA→C57BL/6j) in different strains.The survival time was over 100 days in same strains and (7.10 ±0.74) d (C57BL/6j→BALB/c) ; (7.40 ± 0.82) d (BALB/c→ C57 BL/6j) ; (8.90 ± 1.10) d (C57 BL/6j→ CBA) ; (8.50 ± 0.85) d (CBA→C57BL/6j) d in different strains.It was confirmed with pathological examination.Conclusion On the base of mastering the microsurgical techniques,we can establish the mice cardiac heterotopic transplantation model.The immune response in C57BL/6j to BALB/c model was very strong,while weaker in C57BL/6j to CBA models.The different transplant immune response can be used in the transplantation research.
