CAJ | 학술논문

目的利用层层自组装技术,在钛表面构建精氨酸-甘氨酸-天冬氨酸(RGD)肽/肝素复合涂层,提高其血液相容性,探讨该技术在人工材料表面改性领域的应用前景.方法采用层层自组装技术,在预处理的钛试件表面构建RGD肽/肝素复合涂层;对钛表面涂层进行物理表征分析,评价其体内外血液相容性.结果 RGD肽/肝素复合涂层的体外溶血率为0.76％,该涂层可延长部分活化凝血酶原时间到(24.5±0.6)s,减少纤维蛋白原的吸附,减少血小板的黏附与激活,体内动物实验证实该涂层可减少表面血栓形成.结论利用层层自组装技术形成的RGD肽/肝素复合涂层血液相容性高,有望成为一种新型的生物化钛表面.
목적 이용층층자조장기술,재태표면구건정안산-감안산-천동안산(RGD)태/간소복합도층,제고기혈액상용성,탐토해기술재인공재료표면개성영역적응용전경.방법 채용층층자조장기술,재예처리적태시건표면구건RGD태/간소복합도층;대태표면도층진행물리표정분석,평개기체내외혈액상용성.결과 RGD태/간소복합도층적체외용혈솔위0.76％,해도층가연장부분활화응혈매원시간도(24.5±0.6)s,감소섬유단백원적흡부,감소혈소판적점부여격활,체내동물실험증실해도층가감소표면혈전형성.결론 이용층층자조장기술형성적RGD태/간소복합도층혈액상용성고,유망성위일충신형적생물화태표면.
Objective Arg-Gly-Asp (RGD) peptide/heparin multilayers were coated on pure titanium using a layer-by-layer self-assemble technique,to improve the cytocompatibility of titanium and explore its application in the area of medical metal surface modification.Methods RGD peptide/heparin multilayers were coated on the treated titanium surface.The physical properties of the coating on the titanium surface were analyzed.The in vitro and in vivo blood compatibility of the multilayers was evaluated.Results The ratio of hemolysis of RGD peptide/heparin multilayers was 0.76％,and RGD peptide/heparin multilayers could prolong activated partial thromboplastintime (APTT) to (24.5 ± 0.6) s,and reduce the amount of fibrinogen,and lessen platelet adhesion and activation.It was further confirmed that RGD peptide/heparin multilayers could reduce the surface thrombus formation in the in vivo animal experiment.Conclusion The blood compatibility of pure titanium can be improved significantly after being coated with RGD peptide/heparin multilayers.