雌激素受体β的表达对乳腺癌细胞体内致瘤能力的影响
자격소수체β적표체대유선암세포체내치류능력적영향
Effects on xenografts in nude mice of estrogen receptor β expression
  • 万方数据
    中华实验外科杂志 중화실험외과잡지
    CHINESE JOURNAL OF EXPERIMENTAL SURGERY
    2014年 8期 1700-1701 ,共2页

    刘瑞磊%刘宇%张鹏%黄勇%汤谧%李玺%赖远辉

    류서뢰%류우%장붕%황용%탕밀%리새%뢰원휘

    雌激素受体β%乳腺癌%小干扰RNA%裸鼠 雌激素受體β%乳腺癌%小榦擾RNA%裸鼠
    자격소수체β%유선암%소간우RNA%라서
    Estrogen receptor β%Breast cancer%Small interfering RNA%Nude mice
    目的 观察雌激素受体β(ERβ)的表达对乳腺癌细胞生物学特性以及裸鼠体内致瘤力的影响,探讨其在乳腺癌发生发展中的作用.方法 通过小干扰RNA(siRNA)的方法将ERβ高表达的MCF-7细胞敲降ERβ基因,筛选稳定表达株,利用裸鼠进行体内成瘤性实验,比较肿瘤生长曲线及裸鼠的生存期.结果 siRNA能明显降低乳腺癌细胞MCF-7中ERβ的表达,ERβ被敲降后MCF-7细胞的成瘤能力增强.RNA干扰组在裸鼠皮下接种的第12天开始可触摸到实体肿瘤的形成,对照组第27天开始可触摸到实体肿瘤的形成.RNA干扰组8只裸鼠中位生存时间为33d,对照组8只裸鼠中位生存时间为66 d.结论 ERβ基因对人乳腺癌细胞MCF-7在裸鼠体内皮下成瘤能力有明显的抑制作用.
    목적 관찰자격소수체β(ERβ)적표체대유선암세포생물학특성이급라서체내치류력적영향,탐토기재유선암발생발전중적작용.방법 통과소간우RNA(siRNA)적방법장ERβ고표체적MCF-7세포고강ERβ기인,사선은정표체주,이용라서진행체내성류성실험,비교종류생장곡선급라서적생존기.결과 siRNA능명현강저유선암세포MCF-7중ERβ적표체,ERβ피고강후MCF-7세포적성류능력증강.RNA간우조재라서피하접충적제12천개시가촉모도실체종류적형성,대조조제27천개시가촉모도실체종류적형성.RNA간우조8지라서중위생존시간위33d,대조조8지라서중위생존시간위66 d.결론 ERβ기인대인유선암세포MCF-7재라서체내피하성류능력유명현적억제작용.
    Objective This study was to investigate the effect of estrogen receptor β (ERβ) on tumorigenesis of human breast cancer cell line in nude mice.Methods Breast cancer cell line MCF-7 (high expression of ERβ) was chose to show the tumorigenesis ablity after silence ERβ by small interfering RNA (siRNA).Results siRNA-ERβcan decrease the expression of ERβprotein in MCF-7 cells effectively.After silence ERββby siRNA,the tumorigenesis of MCF-7 cell were significantly enhanced.In ERβ-siRNA group,the tumor was papable on the 12th day,while in control-RNA group,the tumor was papable on the 27th day.The median survival time was 33 days in ERβ-siRNA group and 66 days in control-RNA group.Conclusion The ERβ gene can diminished the xenografts of MCF-7 cells in nudemice obviously.
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