中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
8期
1739-1741
,共3页
郭兴军%王敏%江建新%石程剑%胡均%黄焕军
郭興軍%王敏%江建新%石程劍%鬍均%黃煥軍
곽흥군%왕민%강건신%석정검%호균%황환군
胆管癌%ZO-1%Claudin-1
膽管癌%ZO-1%Claudin-1
담관암%ZO-1%Claudin-1
Cholangiocarcinoma%Zonula occludens-1%Claudin-1
目的 探讨ZO-1及Claudin-1在胆管癌中的表达并分析其与胆管癌侵袭转移的关系.方法 应用免疫组织化学方法检测Claudin-1和ZO-1在胆管癌、肝转移灶和正常胆管组织中的表达;采用实时定量聚合酶链反应(Real-time PCR)方法检测胆管癌细胞株中Claudin-1及ZO-1基因表达水平.结果 Claudin-1与ZO-1在正常胆管组织中的表达量分别是胆管癌组织中的12.94倍和5.19倍(P＜0.01),分别是胆管癌肝转移组织中的125.62倍和60.31倍(P＜0.01);Claudin-1与ZO-1在胆管癌组织中的表达与胆管癌是否发生局部或者远处转移密切相关(P＜0.05).在3种胆管癌细胞株中,Claudin-1和ZO-1在QBC939和RBE中高表达;在TFK-1中低表达.结论 Claudin-1与ZO-1在胆管癌中的表达与胆管癌的侵袭转移能力密切相关.
目的 探討ZO-1及Claudin-1在膽管癌中的錶達併分析其與膽管癌侵襲轉移的關繫.方法 應用免疫組織化學方法檢測Claudin-1和ZO-1在膽管癌、肝轉移竈和正常膽管組織中的錶達;採用實時定量聚閤酶鏈反應(Real-time PCR)方法檢測膽管癌細胞株中Claudin-1及ZO-1基因錶達水平.結果 Claudin-1與ZO-1在正常膽管組織中的錶達量分彆是膽管癌組織中的12.94倍和5.19倍(P＜0.01),分彆是膽管癌肝轉移組織中的125.62倍和60.31倍(P＜0.01);Claudin-1與ZO-1在膽管癌組織中的錶達與膽管癌是否髮生跼部或者遠處轉移密切相關(P＜0.05).在3種膽管癌細胞株中,Claudin-1和ZO-1在QBC939和RBE中高錶達;在TFK-1中低錶達.結論 Claudin-1與ZO-1在膽管癌中的錶達與膽管癌的侵襲轉移能力密切相關.
목적 탐토ZO-1급Claudin-1재담관암중적표체병분석기여담관암침습전이적관계.방법 응용면역조직화학방법검측Claudin-1화ZO-1재담관암、간전이조화정상담관조직중적표체;채용실시정량취합매련반응(Real-time PCR)방법검측담관암세포주중Claudin-1급ZO-1기인표체수평.결과 Claudin-1여ZO-1재정상담관조직중적표체량분별시담관암조직중적12.94배화5.19배(P＜0.01),분별시담관암간전이조직중적125.62배화60.31배(P＜0.01);Claudin-1여ZO-1재담관암조직중적표체여담관암시부발생국부혹자원처전이밀절상관(P＜0.05).재3충담관암세포주중,Claudin-1화ZO-1재QBC939화RBE중고표체;재TFK-1중저표체.결론 Claudin-1여ZO-1재담관암중적표체여담관암적침습전이능력밀절상관.
Objective To study the expression of zonula occludens-1 (ZO-1) and Claudin-1 in cholangiocarcinoma and analyze the relationship between them with invasion and metastasis potency of cholangiocarcinoma.Methods Immunohistochemistry was employed to detect Claudin-1 and ZO-1 expression in the cholangiocarcinoma,liver metastases and normal bile duct tissues.Real-time quantitative polymerase chain reaction (Real-time PCR) was used to detect the expression of Claudin-1 and ZO-1 in three cholangiocarcinoma cell lines (QBC939,RBE,and TFK-1).Results In bile duct,Claudin-1 and ZO-1 protein was localized on the cell membrane and in the cytoplasm.In the cholangiocarcinoma,Claudin-1 and ZO-1 protein expression was significantly reduced,and located in the cytoplasm.In the cholangiocarcinoma liver metastases,Claudin-1 and ZO-1 were not expressed or very weakly expressed.Semi-quantitative analysis found that the expression of ZO-1 in bile duct was stronger than in cholangiocarcinoma (P ＜ 0.01) and cholangiocarcinoma liver metastases (P ＜ 0.01).The expression of Claudin-1 in bile duct was stronger than in cholangiocarcinoma (P ＜ 0.01) and cholangiocarcinoma liver metastases (P ＜ 0.01).In three cholangiocarcinoma cell lines,Claudin-1 and ZO-1 were relatively strongly expressed in RBE and QBC939,and relatively weakly expressed in TFK-1.Conclusion ZO-1 and Claudin-1 expression is relatively downregulated in cholangiocarcinoma tissues and is undetectable in cholangiocarcinoma liver metastasis tissues.
