CAJ | 학술논문

目的观察生物可降解雷帕霉素缓释输尿管支架对输尿管损伤后瘢痕形成的抑制作用.方法原代培养尿路平滑肌细胞,噻唑蓝(MTT)法筛选雷帕霉素抑制尿路平滑肌细胞增殖的最佳药物浓度.再将构建的生物可降解雷帕霉素缓释输尿管支架和双J管分别置入输尿管损伤动物模型,评估两种支架抑制输尿管损伤后瘢痕形成情况.结果 MTT法检测显示雷帕霉素呈现剂量依赖性抑制尿路平滑肌细胞增殖,半数致死量(IC50)为23.3 mg/L.所有支架均成功置入输尿管,18周时,Masson三色和α-平滑肌肌动蛋白(α-SMA)免疫组织化学染色显示生物可降解雷帕霉素缓释输尿管支架侧胶原增生不明显,输尿管壁细胞排列有序,而双J管侧胶原纤维大量增生、层次紊乱.结论生物可降解雷帕霉素缓释输尿管支架能有效抑制尿路平滑肌细胞的过度增殖和胶原沉积,从而预防输尿管瘢痕性狭窄的发生.
목적 관찰생물가강해뢰파매소완석수뇨관지가대수뇨관손상후반흔형성적억제작용.방법 원대배양뇨로평활기세포,새서람(MTT)법사선뢰파매소억제뇨로평활기세포증식적최가약물농도.재장구건적생물가강해뢰파매소완석수뇨관지가화쌍J관분별치입수뇨관손상동물모형,평고량충지가억제수뇨관손상후반흔형성정황.결과 MTT법검측현시뢰파매소정현제량의뢰성억제뇨로평활기세포증식,반수치사량(IC50)위23.3 mg/L.소유지가균성공치입수뇨관,18주시,Masson삼색화α-평활기기동단백(α-SMA)면역조직화학염색현시생물가강해뢰파매소완석수뇨관지가측효원증생불명현,수뇨관벽세포배렬유서,이쌍J관측효원섬유대량증생、층차문란.결론 생물가강해뢰파매소완석수뇨관지가능유효억제뇨로평활기세포적과도증식화효원침적,종이예방수뇨관반흔성협착적발생.
Objective To evaluate the effect of biodegradable rapamycin-eluting ureteral stent in inhibiting scar formation after ureteral injuries.Methods Smooth muscle cells were primary cultured.The inhibitory effect of rapamycin on the proliferation of smooth muscle cells was investigated by methyl thiazol tetrazolium (MTT) detection.And then,the optimal drug concentration was selected to construct drug-eluting stent.Biodegradable rapamycin-eluting ureteral stent and double-J stent were surgically implanted into the traumatic ureteral injury animal model,to evaluate the inhibitory effect in situ by histological tests.Results Smooth muscle cells were successfully cultured in vitro,and the results of MTT confirmed rapamycin could effectively inhibit the proliferation of smooth muscle cells depending on concentration.The IC50 was 23.3 mg/L.All stents were successfully implanted.At 18 weeks,masson trichrome and α-smooth muscle actin (α-SMA) immunohistochemical staining showed that biodegradable rapamycin-eluting ureteral stent,compared with double-J stent,can effectively inhibit the excessive proliferation of smooth muscle cells and collagen deposition.Conclusion Biodegradable rapamycin-eluting ureteral stent can effectively inhibit the excessive proliferation of smooth muscle cells and collagen deposition,thereby preventing ureteral scar stenosis.