中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
8期
1772-1774
,共3页
王鹏%邓军%张延伦%张钢%安刚%张晏
王鵬%鄧軍%張延倫%張鋼%安剛%張晏
왕붕%산군%장연륜%장강%안강%장안
前列腺癌%血管活性肠肽%血管内皮生长因子%微血管密度
前列腺癌%血管活性腸肽%血管內皮生長因子%微血管密度
전렬선암%혈관활성장태%혈관내피생장인자%미혈관밀도
Prostate carcinoma%Vasoactive intestinal peptide%Vascular endothelial growth factor%Microvessel density
目的 检测裸鼠体内激素依赖性前列腺癌(LNCaP)组织中的血管内皮生长因子(VEGF) mRNA含量和微血管密度(MVD),探讨血管活性肠肽(VIP)促血管生成作用的机制.方法 利用雄性小鼠建立经100 nmol/L VIP和未经VIP处理的LNCaP动物模型,前者为实验组,后者为对照组.于第8天和第15天测量移植瘤的体积,利用实时定量聚合酶链反应(Real-time PCR,SYBR染料法)检测移植瘤组织中的VEGF mRNA水平,利用CD34计算移植瘤组织中的MVD.结果 第8天和第15天裸鼠LNCaP移植瘤体积、VEGF mRNA含量、MVD实验组[(55.38±10.82)、(89.41±15.66) mm3;1.38±0.41、1.76 ±0.67;43.7±11.4、59.1±13.8]均大于对照组[(28.56 ±7.39)、(48.73±12.48) mm3;0.42 ±0.13、0.59 ±0.24;26.3 ±9.2、30.6± 10.5],差异均有统计学意义(P＜0.05).结论 VIP对裸鼠LNCaP移植瘤生长起促进作用,可能与其促进肿瘤新生血管相关.
目的 檢測裸鼠體內激素依賴性前列腺癌(LNCaP)組織中的血管內皮生長因子(VEGF) mRNA含量和微血管密度(MVD),探討血管活性腸肽(VIP)促血管生成作用的機製.方法 利用雄性小鼠建立經100 nmol/L VIP和未經VIP處理的LNCaP動物模型,前者為實驗組,後者為對照組.于第8天和第15天測量移植瘤的體積,利用實時定量聚閤酶鏈反應(Real-time PCR,SYBR染料法)檢測移植瘤組織中的VEGF mRNA水平,利用CD34計算移植瘤組織中的MVD.結果 第8天和第15天裸鼠LNCaP移植瘤體積、VEGF mRNA含量、MVD實驗組[(55.38±10.82)、(89.41±15.66) mm3;1.38±0.41、1.76 ±0.67;43.7±11.4、59.1±13.8]均大于對照組[(28.56 ±7.39)、(48.73±12.48) mm3;0.42 ±0.13、0.59 ±0.24;26.3 ±9.2、30.6± 10.5],差異均有統計學意義(P＜0.05).結論 VIP對裸鼠LNCaP移植瘤生長起促進作用,可能與其促進腫瘤新生血管相關.
목적 검측라서체내격소의뢰성전렬선암(LNCaP)조직중적혈관내피생장인자(VEGF) mRNA함량화미혈관밀도(MVD),탐토혈관활성장태(VIP)촉혈관생성작용적궤제.방법 이용웅성소서건립경100 nmol/L VIP화미경VIP처리적LNCaP동물모형,전자위실험조,후자위대조조.우제8천화제15천측량이식류적체적,이용실시정량취합매련반응(Real-time PCR,SYBR염료법)검측이식류조직중적VEGF mRNA수평,이용CD34계산이식류조직중적MVD.결과 제8천화제15천라서LNCaP이식류체적、VEGF mRNA함량、MVD실험조[(55.38±10.82)、(89.41±15.66) mm3;1.38±0.41、1.76 ±0.67;43.7±11.4、59.1±13.8]균대우대조조[(28.56 ±7.39)、(48.73±12.48) mm3;0.42 ±0.13、0.59 ±0.24;26.3 ±9.2、30.6± 10.5],차이균유통계학의의(P＜0.05).결론 VIP대라서LNCaP이식류생장기촉진작용,가능여기촉진종류신생혈관상관.
Objective To explore the mechanism of vasoactive intestinal peptide (VIP) inducing angiogenesis by detecting the vascular endothelial growth factor (VEGF) mRNA content and microvessel density (MVD) in experimental prostate cancer in vivo.Methods Nude mice were subcutaneously injected with LNCaP prostate cancer cells.Cells were incubated for 1 h in the presence (experimental group) or absence (control group) of 100 nmol/L VIP for 1 h before xenograf.By measuring the transplanted tumor volume,real-time quantitative polymerase chain reaction (Real-time PCR) was used to detect VEGF mRNA,and CD34 was applied to calculate the MVD in the transplanted tumor tissue at 8th day and 15th day.Results The tumor volume,VEGF mRNA content and MVD in the experimental group [(55.38 ± 10.82),(89.41 ±15.66) mm3; 1.38±0.41,1.76 ±0.67; 43.7±11.4,59.1 ± 13.8] were significantly increased as compared with those in the control group [(28.56 ±7.39),(48.73 ± 12.48) mm3 ;0.42±0.13,0.59±0.24; 26.3 ±9.2,30.6±10.5; P＜0.05].Conclusion This study indicatesVIP promotes the growth of LNCaP cells probably by inducing angiogenesis in vivo.
