中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
8期
1775-1777
,共3页
王勤%王新峦%邓晔坤%周晓中%秦岭
王勤%王新巒%鄧曄坤%週曉中%秦嶺
왕근%왕신만%산엽곤%주효중%진령
微环境%低剂量射线%成骨特异性转录因子2%骨钙素
微環境%低劑量射線%成骨特異性轉錄因子2%骨鈣素
미배경%저제량사선%성골특이성전록인자2%골개소
The microenvironment%Low dose X-irradiation%Runt-related transcription factor 2%Osteocalcin
目的 观察成骨诱导微环境中低剂量X射线对成骨细胞的影响.方法 照射后分别检测细胞增殖、碱性磷酸酶(ALP)活性、细胞矿化、成骨特异性转录因子2(Runx2)、骨钙素(0C)的mRNA和蛋白表达.结果 1.0Gy组与对照组比较增殖受到抑制(P ＜0.05);ALP活性在0.1Gy组[(5.88 ±0.14) nmol对硝基苯磷酸二钠(pNPP)/μg蛋白]与对照组[(4.75±0.05) nmol pNPP/μg蛋白]比较升高(P＜0.05);矿化定量0.1Gy组(6.57±0.20)与对照组(5.39±0.28)比较升高(P＜0.05);诱导组Runx2基因0.1 Gy组(5.51±1.52)与对照组(2.43±0.3)比较升高(P＜0.05),OC基因0.1 Gy组(2.92±1.34)与对照组(1.03±0.41)比较升高(P＜0.05);诱导组Runx2蛋白表达0.1 Gy组(0.84±0.03)高于对照组(0.44 ±0.02,P＜ 0.05),OC蛋白表达0.1 Gy组[(427.7±64.2)ng/L]高于对照组[(278.9±53.7) ng/L,P＜0.05].结论 成骨诱导微环境在低剂量射线促进成骨细胞的分化和矿化中起重要呈递作用.
目的 觀察成骨誘導微環境中低劑量X射線對成骨細胞的影響.方法 照射後分彆檢測細胞增殖、堿性燐痠酶(ALP)活性、細胞礦化、成骨特異性轉錄因子2(Runx2)、骨鈣素(0C)的mRNA和蛋白錶達.結果 1.0Gy組與對照組比較增殖受到抑製(P ＜0.05);ALP活性在0.1Gy組[(5.88 ±0.14) nmol對硝基苯燐痠二鈉(pNPP)/μg蛋白]與對照組[(4.75±0.05) nmol pNPP/μg蛋白]比較升高(P＜0.05);礦化定量0.1Gy組(6.57±0.20)與對照組(5.39±0.28)比較升高(P＜0.05);誘導組Runx2基因0.1 Gy組(5.51±1.52)與對照組(2.43±0.3)比較升高(P＜0.05),OC基因0.1 Gy組(2.92±1.34)與對照組(1.03±0.41)比較升高(P＜0.05);誘導組Runx2蛋白錶達0.1 Gy組(0.84±0.03)高于對照組(0.44 ±0.02,P＜ 0.05),OC蛋白錶達0.1 Gy組[(427.7±64.2)ng/L]高于對照組[(278.9±53.7) ng/L,P＜0.05].結論 成骨誘導微環境在低劑量射線促進成骨細胞的分化和礦化中起重要呈遞作用.
목적 관찰성골유도미배경중저제량X사선대성골세포적영향.방법 조사후분별검측세포증식、감성린산매(ALP)활성、세포광화、성골특이성전록인자2(Runx2)、골개소(0C)적mRNA화단백표체.결과 1.0Gy조여대조조비교증식수도억제(P ＜0.05);ALP활성재0.1Gy조[(5.88 ±0.14) nmol대초기분린산이납(pNPP)/μg단백]여대조조[(4.75±0.05) nmol pNPP/μg단백]비교승고(P＜0.05);광화정량0.1Gy조(6.57±0.20)여대조조(5.39±0.28)비교승고(P＜0.05);유도조Runx2기인0.1 Gy조(5.51±1.52)여대조조(2.43±0.3)비교승고(P＜0.05),OC기인0.1 Gy조(2.92±1.34)여대조조(1.03±0.41)비교승고(P＜0.05);유도조Runx2단백표체0.1 Gy조(0.84±0.03)고우대조조(0.44 ±0.02,P＜ 0.05),OC단백표체0.1 Gy조[(427.7±64.2)ng/L]고우대조조[(278.9±53.7) ng/L,P＜0.05].결론 성골유도미배경재저제량사선촉진성골세포적분화화광화중기중요정체작용.
Objective To observe the effect of low dose X-irradiation on the proliferation,osteogenic differentiation and mineralization of osteoblastic UMR-106 cells in the osteogenesis microenvironment.Methods After exposure,cell proliferation,osteogenic differentiation and calcium deposition were evaluated by cell counting kit-8 (CCK-8) assay,alkaline phosphatase (ALP) viability,Alizarin Red S staining,respectively.The mRNA and protein levels of Runt-related transcription factor 2 (Runx2) and Osteocalcin (OC) were detected.Results The relative proliferation rate of the cells exposed to 1.0 Gy was significantly lower than that of control (P ＜ 0.05).The ALP activity of 0.1 Gy group [(5.88 ± 0.14) nmol p-Nitrophenyl Phosphate (pNPP)/μg protein] was significantly increased than that of control group [(4.75 ± 0.05) nmol pNPP/μg protein,P ＜ 0.05].The optical density (OD) value of calcium deposition of 0.1 Gy group (6.57 ± 0.20) was significantly increased than that of control group (5.39 ± 0.28) (P ＜0.05).In osteogenesis microenvironment groups,the Runx2 mRNA expression of 0.1 Gy group (5.51 ± 1.52) was significantly increased than that of 0 Gy group (2.43 ± 0.3) (P ＜ 0.05),the OC mRNA expression of 0.1 Gy group (2.92 ± 1.34) was significantly increased than that of 0 Gy group (1.03 ± 0.41) (P ＜ 0.05).In osteogenesis microenvironment groups,the Runx2 protein expression of 0.1 Gy group (0.84 ± 0.03) was significantly increased than that of 0 Gy group (0.44 ± 0.02,P ＜ 0.05),the OC protein expression of 0.1 Gy group (427.7 ± 64.2) ng/L was significantly increased than that of 0 Gy group(278.9 ±53.7) ng/L (P＜0.05).Conclusion The osteogenesis microenvironment plays a present role of low dose X-irradiation promoting osteogenic differentiation and mineralization on osteoblastic cells.
