CAJ | 학술논문

目的探讨骨髓间充质干细胞(BMSCs)与孕酮联合治疗大鼠坐骨神经损伤的疗效.方法将健康成年清洁级雄性SD大鼠100只随机分为假手术组(对照组)和A、B、C、D5组,每组20只,建立大鼠右下肢坐骨神经损伤模型后,A组给予生理盐水,B、C、D组分别给予孕酮(8 mg/kg)、BMSCs移植、BMSCs移植联合应用孕酮(8 mg/kg)修复治疗.术后第12、24、48天采用Basso-Beattie-Bresnahan (BBB)评分法评估各组大鼠的右下肢运动功能恢复情况,术后48 d测定各组大鼠坐骨神经功能指数(SFI),模型建立后0h和48 d取损伤坐骨神经行Western blot分析,检测损伤处脑源性神经营养因子(BDNF)、髓磷脂碱性蛋白(MBP)和生长相关蛋白-43(GAP-43)的蛋白表达水平.结果术后48 d坐骨神经损伤组SFI值均逐渐恢复,D组的SFI值(1.21 ±0.12)明显优于A(12.37±0.28)、B(10.56 ±0.42)、C(6.58 ±0.34)组(P＜0.05).手术当天坐骨神经损伤组的右下肢运动功能完全丧失呈迟缓性瘫痪,D组术后第12、24、48天的运动功能评分(12.65 ±0.95、16.39±1.02、18.19 ±2.00)均明显高于A(8.62±0.77、10.27士0.53、12.58±0.96)、B(9.98±0.81、13.48 ±0.65、15.13±1.28)、C(10.87±0.54、14.06±0.75、16.48±1.96)组,差异有统计学意义(P＜0.05).手术当天A、B、C、D组的BDNF、MBP和GAP-43的蛋白水平较对照组明显降低(P＜0.05);手术后第48天坐骨神经损伤组的上述蛋白水平均明显升高(P＜0.05),其中D组的上述蛋白水平明显高于A、B、C组(P＜0.05).结论 BMSCs联合孕酮治疗能够促进大鼠坐骨神经损伤的神经再生,促进大鼠运动功能的恢复,其机制可能与增加相应的神经组织BDNF、MBP和GAP-43的表达有关.
목적 탐토골수간충질간세포(BMSCs)여잉동연합치료대서좌골신경손상적료효.방법 장건강성년청길급웅성SD대서100지수궤분위가수술조(대조조)화A、B、C、D5조,매조20지,건립대서우하지좌골신경손상모형후,A조급여생리염수,B、C、D조분별급여잉동(8 mg/kg)、BMSCs이식、BMSCs이식연합응용잉동(8 mg/kg)수복치료.술후제12、24、48천채용Basso-Beattie-Bresnahan (BBB)평분법평고각조대서적우하지운동공능회복정황,술후48 d측정각조대서좌골신경공능지수(SFI),모형건립후0h화48 d취손상좌골신경행Western blot분석,검측손상처뇌원성신경영양인자(BDNF)、수린지감성단백(MBP)화생장상관단백-43(GAP-43)적단백표체수평.결과 술후48 d좌골신경손상조SFI치균축점회복,D조적SFI치(1.21 ±0.12)명현우우A(12.37±0.28)、B(10.56 ±0.42)、C(6.58 ±0.34)조(P＜0.05).수술당천좌골신경손상조적우하지운동공능완전상실정지완성탄탄,D조술후제12、24、48천적운동공능평분(12.65 ±0.95、16.39±1.02、18.19 ±2.00)균명현고우A(8.62±0.77、10.27사0.53、12.58±0.96)、B(9.98±0.81、13.48 ±0.65、15.13±1.28)、C(10.87±0.54、14.06±0.75、16.48±1.96)조,차이유통계학의의(P＜0.05).수술당천A、B、C、D조적BDNF、MBP화GAP-43적단백수평교대조조명현강저(P＜0.05);수술후제48천좌골신경손상조적상술단백수평균명현승고(P＜0.05),기중D조적상술단백수평명현고우A、B、C조(P＜0.05).결론 BMSCs연합잉동치료능구촉진대서좌골신경손상적신경재생,촉진대서운동공능적회복,기궤제가능여증가상응적신경조직BDNF、MBP화GAP-43적표체유관.
Objective To investigate the effects of bone marrow mesenchymal stem cells combined with progesterone for repairing sciatic nerve injury in rats.Methods 100 Adult male SD rats were equally divided into control group and groups A,B,C and D (model groups).After rat sciatic nerve injury model was constructed in the model groups,group A,group B,group C and group D were given normal saline,progesterone (8 mg/kg),bone marrow mesenchymal stem cell transplantation,bone marrow mesenchymal stem cells combined with progesterone (8 mg/kg).At 12,24 and 48 day after sciatic nerve injury,functional outcome measurements were evaluated by the Basso-Beattie-Bresnahan (BBB) score.The protein levels of brain derived neurotrophic factor (BDNF),myelin basic protein (MBP) and growth associated protein 43 (GAP-43) were detected by Western blotting after sciatic cord injury at 0 and 48 day.Results After 48 days,SFI values in model groups were gradually restored,but the SFI values in group D (1.21 ± 0.12) were significantly higher than in group A (12.37 ±0.28),group B (10.56 ±0.42) and group C (6.58 ±0.34,P ＜0.05).The dual hind limb motor functions were completely lost with flaccid paralysis in the model groups in the day of surgery.The motor function scores in group D on the postoperative day 12,24,and 48 (12.65 ±0.95,16.39 ± 1.02,and 18.19 ±2.00 respectively) were significantly higher than in group A (8.62 ±0.77,10.27 ±0.53,and 12.58 ±0.96),group B (9.98 ±0.81,13.48 ± 0.65,15.13 ± 1.28) and group C (10.87±0.54,14.06 ±0.75,16.48 ± 1.96,P ＜0.05 for all).The MBP,BDNF and GAP-43 protein levels in the day of surgery in the model groups were significantly lower than in the control group (P ＜ 0.05).These protein levels were significantly higher on the postoperative day 48 (P ＜ 0.05),and those in group D were significantly higher than in groups A,B and C (P ＜ 0.05).Conclusion Bone marrow mesenchymal stem cells combined with progesterone can promote the regeneration of rat sciatic nerve injury and recovery of motor function,which may be associated with the increases of BDNF,MBP and GAP-43 in the corresponding nerve tissues.